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Real-Time Glutamate Release in Rat Striatum of 11-Vessel-Occlusion Ischemia Model Treated with Acupuncture  

Yin, Chang-Shik (Acupuncture and Meridian Science Research Center, Kyung Hee University)
Choi, Seok-Keun (Department of Neurosurgery, College of Meridian, Kyung Hee University)
Lee, Gi-Ja (Department of Biomedical Engineering, College of Meridian, Healthcare Industry Research Institute, Kyung Hee University)
Eo, Yun-Hye (Department of Biomedical Engineering, College of Meridian, Healthcare Industry Research Institute, Kyung Hee University)
Kim, Bum-Shik (Healthcare Industry Research Institute)
Oh, Berm-Seok (Department of Biomedical Engineering, College of Meridian, Healthcare Industry Research Institute, Program of Medical Engineering, Kyung Hee University)
Lim, Ji-Eun (Department of Biomedical Engineering, College of Meridian, Healthcare Industry Research Institute, Kyung Hee University)
Lee, Hye-Jung (Acupuncture and Meridian Science Research Center, Department of Meridian & Acupuncture College of Oriental Meridian, Kyung Hee University)
Park, Hun-Kuk (Department of Biomedical Engineering, College of Meridian, Healthcare Industry Research Institute, Program of Medical Engineering, Kyung Hee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.4, 2008 , pp. 835-840 More about this Journal
Abstract
Acupuncture has long been contended to be effective in an ischemic stroke. A real-time monitoring of glutamate, an excitotoxin in the process of ischemic neuronal damage, in the striatum is tried in a rat model of global ischemia. Global ischemia was induced by the 11 vessel occlusion method for 10 minutes, during which acupuncture stimulation on GB34 and GB39 points was executed. Glutamate release in the rat striatum was monitored 256 times per second using real-time amperometric biosensor. Real time measurement data of 10 minutes prior to the induction of ischemia served as baseline data. Data acquisition continued for 30 minutes after the initiation of reperfusion. Peak concentration of glutamate release along with incidentally measured EEG and cerebral blood flow was compared between cases with and without acupuncture stimulation. Peak concentration of glutamate lowered when acupuncture stimulation was executed. A real time monitoring system of 11 vessel-occlusion induced global ischemia model was successfully established. The effect by acupuncture on acute global ischemia was successfully observed in this real-time monitoring setting, which may be one of the neuroprotective mechanism of acupuncture.
Keywords
acupuncture; glutamate; Real-time monitor; ischemia; 11 vessel occlusion model;
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