한국양식학회지 (Journal of Aquaculture)

한국양식학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지

Diluents and Cryoprotectants for Cryopreservation of Filefish Thamnaconus modestus Sperm

  • Le, Minh-Hoang (Department of Aquaculture, Pukyong National University) ;
  • Lim, Han-Kyu (Aquaculture Management Team, National Fisheries Research and Development Institute) ;
  • Min, Byung-Hwa (Aquaculture Management Team, National Fisheries Research and Development Institute) ;
  • Son, Maeng-Hyun (Aquaculture Management Team, National Fisheries Research and Development Institute) ;
  • Lee, Jung-Uie (Aquaculture Management Team, National Fisheries Research and Development Institute) ;
  • Chang, Young-Jin (Department of Aquaculture, Pukyong National University)
  • 발행 : 2008.02.25
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초록

The present study aimed to find the best diluent and cryoprotectant for sperm cryopreservation of filefish Thamnaconus modestus. Two kinds of artificial seminal plasma(ASP1, ASP2), 0.3 M glucose and marine fish Ringer's solution(MFRS) were employed as diluent. Dimethyl sulfoxide(DMSO) and methanol as cryoprotectant were selected for sperm cryopreservation. Sperm was diluted at the ratio of 1:3 with diluents containing cryoprotectants and adjusted for final concentration at 5%, 10%, 15% and 20%. Mixed milt was frozen at liquid nitrogen vapor after equilibration for 5 min. The highest motility($40.5{\pm}2.8%$) and swimming speed($81.5{\pm}4.1{\mu}m/s$) of frozen/thawed sperm were observed in ASP1 diluent containing 10% DMSO and in ASP2 containing 15% DMSO, respectively. Results showed that cryopreservation with ASP as diluent and DMSO as cryoprotectant could be adopted for long term storage of filefish sperm.

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참고문헌

  1. Aoki, K., M. Okamoto, K. Tatsumi and Y. Ishikawa, 1997. Cryopreservation of medaka spermatozoa. Zool. Sci., 14, 641− 644 https://doi.org/10.2108/zsj.14.641
  2. A., G. Vandenberg, D. Proulx and J. L. Bailey, 2002. Comparison of extenders, dilution ratios and theophylline addition on the function of cryopreserved walleye semen. Theriogenology, 57, 1061−1071
  3. Blaxter, J. H. S., 1953. Sperm storage and cross-fertilization of spring and autumn spawning herring. Nature, 172, 1189−1190
  4. Cabrita, E., R. Alvarez, L. Anel, K. J. Rana and M. P. Herraez, 1998. Sublethal damage during cryopreservation of rainbow trout sperm. Cryobiology, 37, 245−253
  5. Chang, Y. J., 1997. Present and future studies on the cryopreservation of fish gametes. Suisanzoshoku, 45, 557−564
  6. Chang, Y. J., H. K. Lim, Y. J. Chang and H. S. Kim, 1999a. Sperm cryopreservation and fertility of post-thaw sperm in river puffer, Takifugu obscrus. J. Aquaculture, 12, 1−5. (in Korean)
  7. Chang, Y. J., Y. H. Choi, H. K. Lim and K. H. Kho, 1999b. Cold storage and cryopreservation of grey mullet (Mugil cephalus) sperm. J. Aquacult., 12, 57−62. (in Korean)
  8. Chao, N. H., W. C. Chao, K. C. Liu and I. C. Liao, 1987. The properties of tilapia sperm and its cryopreservation. J. Fish Biol., 30, 107−118
  9. Chao, N. H. and I. C. Liao, 2001. Cryopreservation of finfish and shellfish gametes and embryos. Aquaculture, 197, 161−189
  10. Chao, N. H., H. P. Chen and I. C. Liao, 1975. Study on cryogenic preservation of grey mullet sperm. Aquaculture, 5, 389−406
  11. Chen, S. L., X. T. Liu, and C. J. Fu, 1993. Cryopreservation of Chinese carp spermatozoa. Aquaculture, 111, 324−325
  12. Drokin, S., H. Stein and H. Brtscherer, 1998. Effect of cryopreservation on the fine structure of spermatozoa of rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta F. fario). Cryobiology, 37, 263−270
  13. Figiel, C. R. and T. R. Tiersch, 1997. Comprehenive literature review of fish sperm cryopreervation in Abstracts of World Aquaculture 1997, Washington, USA
  14. Gwo, J. C., K. Strawn, M. T. Longnecker and C. R. Arnold, 1991. Cryopreservation of Atlantic croaker spermatozoa. Aquaculture, 94, 355−375
  15. Harvey, B., 1983. Cryopreservation of Sarotherodon mossambicus spermatozoa. Aquaculture, 32, 313−320
  16. Harvey, B., R. N. Kelley and M. J. Ashwood-Smith, 1982. Cryopreservation of zebrafish spermatozoa using methanol. Can. J. Zool., 60, 1867−1870
  17. Jamieson, B. G. M., 1991. Fish Evolution and Systematics: Evidence from Spermatozoa. Cambridge University Press, New York, 319 pp
  18. Kurokura H. and R. Hirano, 1980. Cryopreservation of rainbow trout sperm. Bull. Jap. Soc. Sci. Fish., 46, 1493−1495
  19. Kusuda, S., 2004. Current status and perspective of cryopreservation of sperm and blastomeres in fish. Fish Gen. Breed. Sci., 34, 1−25. (in Japanese)
  20. Labbe, C. and G. Maisse, 1996. Influence of rainbow trout thermal acclimation on sperm cryopreservation: relation to change in the lipod composition of the plasma membrane. Aquaculture, 145, 281−294
  21. Lahnsteiner, F., T. Weismann and R.A. Patzner, 1997. Methanol as cryoprotectant and the suitability of 1.2 mL and 5 mL straws for cryopreservation of semen from salmonid fishes. Aquacult. Res., 28, 471−479
  22. Lim, H. K., C. M. An, G. A. Noh and B. H. Min, 2007. Effect of diluents and cryoprotectants on sperm cryopreservation in starry flounder (Platichthys stellatus). J. Aquacult., 20, 173− 177. (in Korean)
  23. Lubzens E., N. Daube, I. Pekarsky, Y. Magnus, A. Cohen, F. Yusefovich and P. Feigin, 1997. Carp (Cyprinus carpio L.) spermatozoa cryobanks - strategies in research and application. Aquaculture, 155, 13−30
  24. McNiven, M. A., R. K. Gallant and G. F. Richardson, 1993. Dimethyl- acetamide as cryoprotectants for rainbow trout spermatozoa. Theriogenology, 40, 943−948
  25. Steyn, G. J., 1993. The effect of freezing rate on the survival of cryopreserved African sharptooth catfish (Clarias gariepinus) spermatozoa. Cryobiology, 30, 581−590
  26. Stoss, J. and T. Refstie, 1983. Short-term storage and cryopreservation of mit from Atlantic salmon and sea trout. Aquaculture, 30, 229−236
  27. Suquet, M., C. Dreanno, C. Fauvel, J. Cosson and R. Billard, 2000. Cryopreservation of sperm in marine fish. Aquacult. Res., 31, 231−243
  28. Tiersch, T. R., 2000. Introduction. In: Cryopreservation in Aquatic Species (ed. By T. R. Tiersch and P. M. Mazik), pp. 19−26. World Aquaculture Society, Baton Rouge, LA, USA
  29. Tiersch, T. R., C. A. Goudie and G. J. Carmichael, 1994. Cryopreservation of channel catfish sperm: storage in cryoprotectants, fertilization trials and growth of channel catfish produced with cryopreserved sperm. Trans. Am. Fish. Soc., 123, 580−586
  30. Wheeler, P. A. and G. H. Thorgaard, 1991. Cryopreservation of rainbow trout semen in large straws. Aquaculture, 93, 95−100
  31. Young, J. A., M. F. Capra and A. W. Blackshaw, 1992. Cryopreservation of summer whiting (Sillago ciliata) spermatozoa. Aquaculture, 102, 155−160