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Diluents and Cryoprotectants for Cryopreservation of Filefish Thamnaconus modestus Sperm  

Le, Minh-Hoang (Department of Aquaculture, Pukyong National University)
Lim, Han-Kyu (Aquaculture Management Team, National Fisheries Research and Development Institute)
Min, Byung-Hwa (Aquaculture Management Team, National Fisheries Research and Development Institute)
Son, Maeng-Hyun (Aquaculture Management Team, National Fisheries Research and Development Institute)
Lee, Jung-Uie (Aquaculture Management Team, National Fisheries Research and Development Institute)
Chang, Young-Jin (Department of Aquaculture, Pukyong National University)
Publication Information
Journal of Aquaculture / v.21, no.1, 2008 , pp. 54-59 More about this Journal
Abstract
The present study aimed to find the best diluent and cryoprotectant for sperm cryopreservation of filefish Thamnaconus modestus. Two kinds of artificial seminal plasma(ASP1, ASP2), 0.3 M glucose and marine fish Ringer's solution(MFRS) were employed as diluent. Dimethyl sulfoxide(DMSO) and methanol as cryoprotectant were selected for sperm cryopreservation. Sperm was diluted at the ratio of 1:3 with diluents containing cryoprotectants and adjusted for final concentration at 5%, 10%, 15% and 20%. Mixed milt was frozen at liquid nitrogen vapor after equilibration for 5 min. The highest motility($40.5{\pm}2.8%$) and swimming speed($81.5{\pm}4.1{\mu}m/s$) of frozen/thawed sperm were observed in ASP1 diluent containing 10% DMSO and in ASP2 containing 15% DMSO, respectively. Results showed that cryopreservation with ASP as diluent and DMSO as cryoprotectant could be adopted for long term storage of filefish sperm.
Keywords
Thamnaconus modestus; Filefish; Cryopreservation; Diluent; Cryoprotectant;
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1 Aoki, K., M. Okamoto, K. Tatsumi and Y. Ishikawa, 1997. Cryopreservation of medaka spermatozoa. Zool. Sci., 14, 641− 644   DOI   ScienceOn
2 Chang, Y. J., H. K. Lim, Y. J. Chang and H. S. Kim, 1999a. Sperm cryopreservation and fertility of post-thaw sperm in river puffer, Takifugu obscrus. J. Aquaculture, 12, 1−5. (in Korean)
3 Chang, Y. J., Y. H. Choi, H. K. Lim and K. H. Kho, 1999b. Cold storage and cryopreservation of grey mullet (Mugil cephalus) sperm. J. Aquacult., 12, 57−62. (in Korean)
4 Figiel, C. R. and T. R. Tiersch, 1997. Comprehenive literature review of fish sperm cryopreervation in Abstracts of World Aquaculture 1997, Washington, USA
5 Gwo, J. C., K. Strawn, M. T. Longnecker and C. R. Arnold, 1991. Cryopreservation of Atlantic croaker spermatozoa. Aquaculture, 94, 355−375
6 Labbe, C. and G. Maisse, 1996. Influence of rainbow trout thermal acclimation on sperm cryopreservation: relation to change in the lipod composition of the plasma membrane. Aquaculture, 145, 281−294
7 Lahnsteiner, F., T. Weismann and R.A. Patzner, 1997. Methanol as cryoprotectant and the suitability of 1.2 mL and 5 mL straws for cryopreservation of semen from salmonid fishes. Aquacult. Res., 28, 471−479
8 Lim, H. K., C. M. An, G. A. Noh and B. H. Min, 2007. Effect of diluents and cryoprotectants on sperm cryopreservation in starry flounder (Platichthys stellatus). J. Aquacult., 20, 173− 177. (in Korean)   과학기술학회마을
9 Harvey, B., R. N. Kelley and M. J. Ashwood-Smith, 1982. Cryopreservation of zebrafish spermatozoa using methanol. Can. J. Zool., 60, 1867−1870
10 Wheeler, P. A. and G. H. Thorgaard, 1991. Cryopreservation of rainbow trout semen in large straws. Aquaculture, 93, 95−100
11 Lubzens E., N. Daube, I. Pekarsky, Y. Magnus, A. Cohen, F. Yusefovich and P. Feigin, 1997. Carp (Cyprinus carpio L.) spermatozoa cryobanks - strategies in research and application. Aquaculture, 155, 13−30
12 Chao, N. H., H. P. Chen and I. C. Liao, 1975. Study on cryogenic preservation of grey mullet sperm. Aquaculture, 5, 389−406
13 Stoss, J. and T. Refstie, 1983. Short-term storage and cryopreservation of mit from Atlantic salmon and sea trout. Aquaculture, 30, 229−236
14 Drokin, S., H. Stein and H. Brtscherer, 1998. Effect of cryopreservation on the fine structure of spermatozoa of rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta F. fario). Cryobiology, 37, 263−270
15 Cabrita, E., R. Alvarez, L. Anel, K. J. Rana and M. P. Herraez, 1998. Sublethal damage during cryopreservation of rainbow trout sperm. Cryobiology, 37, 245−253
16 Blaxter, J. H. S., 1953. Sperm storage and cross-fertilization of spring and autumn spawning herring. Nature, 172, 1189−1190
17 Jamieson, B. G. M., 1991. Fish Evolution and Systematics: Evidence from Spermatozoa. Cambridge University Press, New York, 319 pp
18 Tiersch, T. R., 2000. Introduction. In: Cryopreservation in Aquatic Species (ed. By T. R. Tiersch and P. M. Mazik), pp. 19−26. World Aquaculture Society, Baton Rouge, LA, USA
19 Chao, N. H. and I. C. Liao, 2001. Cryopreservation of finfish and shellfish gametes and embryos. Aquaculture, 197, 161−189
20 Kurokura H. and R. Hirano, 1980. Cryopreservation of rainbow trout sperm. Bull. Jap. Soc. Sci. Fish., 46, 1493−1495
21 Young, J. A., M. F. Capra and A. W. Blackshaw, 1992. Cryopreservation of summer whiting (Sillago ciliata) spermatozoa. Aquaculture, 102, 155−160
22 Tiersch, T. R., C. A. Goudie and G. J. Carmichael, 1994. Cryopreservation of channel catfish sperm: storage in cryoprotectants, fertilization trials and growth of channel catfish produced with cryopreserved sperm. Trans. Am. Fish. Soc., 123, 580−586
23 Kusuda, S., 2004. Current status and perspective of cryopreservation of sperm and blastomeres in fish. Fish Gen. Breed. Sci., 34, 1−25. (in Japanese)
24 A., G. Vandenberg, D. Proulx and J. L. Bailey, 2002. Comparison of extenders, dilution ratios and theophylline addition on the function of cryopreserved walleye semen. Theriogenology, 57, 1061−1071
25 Steyn, G. J., 1993. The effect of freezing rate on the survival of cryopreserved African sharptooth catfish (Clarias gariepinus) spermatozoa. Cryobiology, 30, 581−590
26 Chao, N. H., W. C. Chao, K. C. Liu and I. C. Liao, 1987. The properties of tilapia sperm and its cryopreservation. J. Fish Biol., 30, 107−118
27 Harvey, B., 1983. Cryopreservation of Sarotherodon mossambicus spermatozoa. Aquaculture, 32, 313−320
28 McNiven, M. A., R. K. Gallant and G. F. Richardson, 1993. Dimethyl- acetamide as cryoprotectants for rainbow trout spermatozoa. Theriogenology, 40, 943−948
29 Chen, S. L., X. T. Liu, and C. J. Fu, 1993. Cryopreservation of Chinese carp spermatozoa. Aquaculture, 111, 324−325
30 Chang, Y. J., 1997. Present and future studies on the cryopreservation of fish gametes. Suisanzoshoku, 45, 557−564
31 Suquet, M., C. Dreanno, C. Fauvel, J. Cosson and R. Billard, 2000. Cryopreservation of sperm in marine fish. Aquacult. Res., 31, 231−243