Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cell Recycled Culture of Succinic Acid-Producing Anaerobiospirillum succiniciproducens Using an Internal Membrane Filtration System

  • Lee, Pyung-Cheon (Department of Molecular Science and Technology and Department of Biotechnology, Ajou University) ;
  • Lee, Sang-Yup (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Chang, Ho-Nam (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
  • Published : 2008.07.31
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Abstract

Cell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically carried out using an internal membrane filter module in order to examine the physiological response of A. succiniciproducens to a high-cell-density environment. The optimal growth of A. succiniciproducens and its enhanced succinic acid productivity were observed under $CO_2$-rich conditions, established by adding $NaHCO_3$ and $Na_2CO_3$, in the cell recycled system. A. succiniciproducens grew up to 6.50 g-DCW/l, the highest cell concentration obtained so far, in cell recycled cultures. The cells did not change their morphology, which is known to be easily changed in unfavorable or stress environments. The maximum productivity of succinic acid was about 3.3 g/l/h, which is 3.3 times higher than those obtained in batch cultures. These results can serve as a guide for designing highly efficient cell recycled systems for succinic acid at a commercial level.

Keywords

References

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