Browse > Article

Cell Recycled Culture of Succinic Acid-Producing Anaerobiospirillum succiniciproducens Using an Internal Membrane Filtration System  

Lee, Pyung-Cheon (Department of Molecular Science and Technology and Department of Biotechnology, Ajou University)
Lee, Sang-Yup (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
Chang, Ho-Nam (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.7, 2008 , pp. 1252-1256 More about this Journal
Abstract
Cell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically carried out using an internal membrane filter module in order to examine the physiological response of A. succiniciproducens to a high-cell-density environment. The optimal growth of A. succiniciproducens and its enhanced succinic acid productivity were observed under $CO_2$-rich conditions, established by adding $NaHCO_3$ and $Na_2CO_3$, in the cell recycled system. A. succiniciproducens grew up to 6.50 g-DCW/l, the highest cell concentration obtained so far, in cell recycled cultures. The cells did not change their morphology, which is known to be easily changed in unfavorable or stress environments. The maximum productivity of succinic acid was about 3.3 g/l/h, which is 3.3 times higher than those obtained in batch cultures. These results can serve as a guide for designing highly efficient cell recycled systems for succinic acid at a commercial level.
Keywords
Anaerobiospirillum succiniciproducens; succinic acid; membrane filtration; continuous culture;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 12  (Related Records In Web of Science)
연도 인용수 순위
1 Bazaes, S., M. Toncio, M. Laivenieks, J. G. Zeikus, and E. Cardemil. 2007. Comparative kinetic effects of Mn (II), Mg (II) and the ATP/ADP ratio on phosphoenolpyruvate carboxykinases from Anaerobiospirillum succiniciproducens and Saccharomyces cerevisiae. Protein J. 26: 265-269   DOI   ScienceOn
2 Davis, C. P., D. Cleven, J. Brown, and E. Balish. 1976. Anaerobiospirillum, a new genus of spiral-shaped bacteria. Int. J. Syst. Bacteriol. 26: 498-504   DOI
3 Jabalquinto, A. M., F. D. Gonzalez-Nilo, M. Laivenieks, M. Cabezas, J. G. Zeikus, and E. Cardemil. 2004. Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase. Mutagenesis at metal site 1. Biochimie 86: 47-51   DOI   ScienceOn
4 Kim, T. Y., H. U. Kim, J. M. Park, H. Song, J. S. Kim, and S. Y. Lee. 2007. Genome-scale analysis of Mannheimia succiniciproducens metabolism. Biotechnol. Bioeng. 97: 657-671   DOI   ScienceOn
5 Kwon, S., I. K. Yoo, W. G. Lee, H. N. Chang, and Y. K. Chang. 2001. High-rate continuous production of lactic acid by Lactobacillus rhamnosus in a two-stage membrane cell-recycle bioreactor. Biotechnol. Bioeng. 73: 25-34   DOI   ScienceOn
6 Kwon, Y. D., S. Y. Lee, and P. Kim. 2006. Influence of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) expression on succinic acid fermentation in Escherichia coli under high bicarbonate condition. J. Microbiol. Biotechnol. 16: 1448-1452   과학기술학회마을
7 Lee, P. C., S. Y. Lee, S. H. Hong, and H. N. Chang. 2002. Isolation and characterization of a new succinic acid-producing bacterium, Mannheimia succiniciproducens MBEL55E, from bovine rumen. Appl. Microbiol. Biotechnol. 58: 663-668   DOI   ScienceOn
8 Lee, P. C., W. G. Lee, S. Y. Lee, and H. N. Chang. 1999. Effects of medium components on the growth of Anaerobiospirillum succiniciproducens and succinic acid production. Process Biochem. 35: 49-55   DOI   ScienceOn
9 Park, B. G., W. G. Lee, Y. K. Chang, and H. N. Chang. 1997. Effects of periodic backflushing with filtrate on filtration performance in an internal-filtration bioreactor. Bioprocess Eng. 16: 253-256   DOI   ScienceOn
10 Lee, P. C., S. Y. Lee, S. H. Hong, H. N. Chang, and S. C. Park. 2003. Biological conversion of wood hydrolysate to succinic acid by Anaerobiospirillum succiniciproducens. Biotechnol. Lett. 25: 111-114   DOI   ScienceOn
11 Landucci, R., B. Goodman, and C. Wyman. 1994. Methodology for evaluating the economics of biologically producing chemicals and materials from alternative feedstocks. Appl. Biochem. Biotechnol. 45: 677-696   DOI   ScienceOn
12 Lynd, L. R., C. E. Wyman, and T. U. Gerngross. 1999. Biocommodity engineering. Biotechnol. Prog. 15: 777-793   DOI   ScienceOn
13 Kang, B. C., S. Y. Lee, and H. N. Chang. 1993. Production of Bacillus thuringiensis spores in total cell retention culture and 2-stage continuous-culture using an internal ceramic filter system. Biotechnol. Bioeng. 42: 1107-1112   DOI   ScienceOn
14 Lee, W. G., B. G. Park, Y. K. Chang, H. N. Chang, J. S. Lee, and S. C. Park. 2000. Continuous ethanol production from concentrated wood hydrolysates in an internal membranefiltration bioreactor. Biotechnol. Prog. 16: 302-304   DOI   ScienceOn
15 Guettler, M. V., D. Rumler, and M. K. Jain. 1999. Actinobacillus succinogenes sp. nov., a novel succinic-acidproducing strain from the bovine rumen. Int. J. Syst. Bacteriol. 49: 207-216   DOI   ScienceOn
16 Kim, J., C. Park, S. W. Kim, and S. Kim. 2006. Flux optimization using genetic algorithms in membrane bioreactor. J. Microbiol. Biotechnol. 16: 863-869   과학기술학회마을
17 Thakker, C., S. Bhosale, and D. Ranade. 2006. Formation of succinic acid by Klebsiella pneumoniae MCM B-325 under aerobic and anaerobic conditions. J. Microbiol. Biotechnol. 16: 870-879   과학기술학회마을
18 Lee, P. C., W. G. Lee, S. Kwon, S. Y. Lee, and H. N. Chang. 1999. Succinic acid production by Anaerobiospirillum succiniciproducens: Effects of the H2/CO2 supply and glucose concentration. Enzyme Microb. Technol. 24: 549-554   DOI   ScienceOn
19 Lee, W. G., Y. S. Lee, H. N. Chang, and Y. K. Chang. 1994. A cell retention internal filter reactor for ethanol-production using tapioca hydrolysates. Biotechnol. Tech. 8: 817-820   DOI   ScienceOn
20 Zeikus, J. G., M. K. Jain, and P. Elankovan. 1999. Biotechnology of succinic acid production and markets for derived industrial products. Appl. Microbiol. Biotechnol. 51: 545-552   DOI   ScienceOn
21 Yoo, I. K., K. J. Lim, W. S. Lee, D. J. Kim, and G. C. Cha. 2006. Study on operational factors in a nitrite-accumulating submerged membrane bioreactor. J. Microbiol. Biotechnol. 16: 469-474   과학기술학회마을
22 Samuelov, N. S., R. Datta, M. K. Jain, and J. G. Zeikus. 1999. Whey fermentation by Anaerobiospirillum succiniciproducens for production of a succinate-based animal feed additive. Appl. Environ. Microbiol. 65: 2260-2263
23 Chang, H. N., I. K. Yoo, and B. S. Kim. 1994. High-density cell-culture by membrane-based cell recycle. Biotechnol. Adv. 12: 467-487   DOI   ScienceOn
24 Gokarn, R. R., M. A. Eiteman, and J. Sridhar. 1997. Production of succinate by anaerobic microorganisms. Am. Chem. Soc. Symp. Ser. 666: 237-253
25 Nghiem, N. P., B. H. Davison, B. E. Suttle, and G. R. Richardson. 1997. Production of succinic acid by Anaerobiospirillum succiniciproducens. Appl. Biochem. Biotechnol. 63: 565-576   DOI   ScienceOn
26 Hong, S. H., J. S. Kim, S. Y. Lee, Y. H. In, S. S. Choi, J. K. Rih, C. H. Kim, H. Jeong, C. G. Hur, and J. J. Kim. 2004. The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens. Nat. Biotechnol. 22: 1275-1281   DOI   ScienceOn
27 Lee, P. C., W. G. Lee, S. Kwon, S. Y. Lee, and H. N. Chang. 2000. Batch and continuous cultivation of Anaerobiospirillum succiniciproducens for the production of succinic acid from whey. Appl. Microbiol. Biotechnol. 54: 23-27   DOI   ScienceOn
28 Lee, P. C., W. G. Lee, S. Y. Lee, and H. N. Chang. 2001. Succinic acid production with reduced by-product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source. Biotechnol. Bioeng. 72: 41-48   DOI   ScienceOn