Analysis of Oxalic Acid of Various Vegetables Consumed in Korea

  • Kim, Dae-Jung (Department of Interdisciplinary Program of Biotechnology Industry, Chungbuk National University) ;
  • Kim, Hoi-Kyung (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Myung-Hee (School of Food Technology and Food Service Industry, Yeungnam University) ;
  • Lee, Jun-Soo (Department of Food Science and Technology, Chungbuk National University)
  • Published : 2007.08.31

Abstract

Many vegetables contain oxalate at various levels depending on their type and family. Oxalate is known to reduce mineral bioavailability from foods. The following study was conducted to determine soluble and total oxalate contents in 32 plant samples commonly consumed in Korea using high-performance liquid chromatography (HPLC). Chard, amaranth, and spinach contained total oxalate of >1,000 mg/100 g. Approximately 45% of the oxalate in amaranth is insoluble, whereas 74.46 and 92.45% of the oxalates in chard and spinach, respectively, was soluble oxalates, which may be removed by blanching. Eggplant, carrot, leak, ginger root, spinach, burdock, and sweet pepper contained more than 90% soluble oxalate in total oxalate content. However, all oxalates detected in lettuce and celery were insoluble. Oxalate was not detected in shepherd's purse, bellflower root, garlic, radish root, broccoli, cabbage, onion, lotus root, adlay, cucumber, kale, and pumpkin. These observations provide useful information needed for selection of vegetables.

Keywords

References

  1. Massey LK, Roman-Smith H, Sutton RA. Effect of dietary oxalate and calcium on urinary oxalate and risk of formation of calcium oxalate kidney stones. J. Am. Diet Assoc. 93: 901-906 (1993) https://doi.org/10.1016/0002-8223(93)91530-4
  2. McConn MM, Nakata PA. Oxalate reduces calcium availability in the pads of the prickly pear cactus through formation of calcium oxalate crystals. J. Agr. Food Chem. 52: 1371-1374 (2004) https://doi.org/10.1021/jf035332c
  3. Ross AB, Savage GP, Martin RJ, Vanhanen L. Oxalates in Oca (New Zealand yam)(Oxalis tuberose Mol.). J. Agr. Food Chem. 47: 5019-5022 (1999) https://doi.org/10.1021/jf990332r
  4. Noonan SC, Savage GP. Oxalate content of foods and its effect on humans. Asia Pac. J. Clin. Nutr. 8: 64-74 (1999)
  5. Grazyna J. Content of nitrates, nitrites, and oxalates in New Zealand spinach. Food Chem. 89: 235-242 (2005) https://doi.org/10.1016/j.foodchem.2004.02.030
  6. Holloway WD, Argall ME, Jealous WT, Lee JA, Bradbury JH. Organic acids and calcium oxalate in tropical root crops. J. Agr. Food Chem. 37: 337-341 (1989) https://doi.org/10.1021/jf00086a014
  7. Mosha TC, Gaga HE, Pace RD, Laswai HS, Mtebe K. Effect of blanching on the content of antinutritional factors in selected vegetables. Plant Food Hum. Nutr. 47: 361-367 (1995) https://doi.org/10.1007/BF01088275
  8. Liebman M, Chai WW. Oxalate content of legumes, nuts, and grainbased flours. J. Food Compos. Anal. 18: 723-729 (2005) https://doi.org/10.1016/j.jfca.2004.07.001
  9. Libert B, Franceschi VR. Oxalate in crop plants. J. Agr. Food Chem. 35: 926-938 (1987) https://doi.org/10.1021/jf00078a019
  10. Savage GP, Vanhanen L, Mason SM, Ross AB. Effect of cooking on the soluble and insoluble oxalate content of some New Zealand foods. J. Food Compos. Anal. 13: 201-206 (2000) https://doi.org/10.1006/jfca.2000.0879
  11. Baker CJL. The determination of oxalates in fresh plant material. Analyst 77: 340-344 (1952) https://doi.org/10.1039/an9527700340
  12. Zarembski PM, Hodgkinson A. The oxalic acid content of English diets. Brit. J. Nutr. 16: 627-634 (1962) https://doi.org/10.1079/BJN19620061
  13. Judprasong K, Charoenkiatkul S, Sungpuang P, Vasanachit K, Nakjamanong Y. Total and soluble oxalate contents in Thai vegetables, cereal grains, and legume seeds and their changes after cooking. J. Food Compos. Anal. 19: 340-347 (2006) https://doi.org/10.1016/j.jfca.2005.04.002
  14. Honow R, Bongartz D, Hesse A. An improved HPLC-enzymereactor method for the determination of oxalic acid in complex matrices. Clin. Chim. Acta 261: 131-139 (1997) https://doi.org/10.1016/S0009-8981(97)06521-2
  15. Lee SM, Lee J. Tocopherol and tocotrienol contents of vegetable oils, margarines, butters, and peanut butters consumed in the Korean diet. Food Sci. Biotechnol. 15: 183-188 (2006)
  16. U.S. Department of Agriculture, Agricultural Research Service. USDA composition of foods, vegetables, and vegetable products. p.11. In: Agriculture Handbook. No. 11. Human Nutrition Information Service, Washington, DC, USA (1984)
  17. Honow R, Hesse A. Comparison of extraction methods for the determination of soluble and total oxalates in foods by HPLCenzyme- reactor. Food Chem. 78: 511-521 (2002) https://doi.org/10.1016/S0308-8146(02)00212-1
  18. Albihn PB, Savage GP. The bioavailability of oxalate from Oca (Oxalis tuberosa). J. Urology 166: 420-422 (2001) https://doi.org/10.1016/S0022-5347(05)65956-3
  19. Chawla S, Saxena A, Seshadri S. In-vitro availability of iron in various green leafy vegetables. J. Sci. Food Agr. 46: 125-127 (1988) https://doi.org/10.1002/jsfa.2740460112
  20. Chen CL, Fang HC, Chou KJ, Wang JS, Chung HM. Acute oxalate nephropathy after ingestion of star fruit. Am. J. Kidney Dis. 37: 418-422 (2001) https://doi.org/10.1053/ajkd.2001.21333