Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Comparison of Genetic Diversity of Saxifraga Species Distributed in the Arctic Svalbard and Korea

북극권 Svalbard 지역과 한국에 분포하는 Saxifraga 속 식물의 유전적 다형성 비교

  • 서효원 (농촌진흥청 고령지농업연구소) ;
  • 강성호 (한국해양연구원 부설 극지연구소) ;
  • 이정윤 (농촌진흥청 고령지농업연구소) ;
  • 박영은 (농촌진흥청 고령지농업연구소) ;
  • 조지홍 (농촌진흥청 고령지농업연구소) ;
  • 안원경 (농촌진흥청 고령지농업연구소) ;
  • 유동림 (농촌진흥청 고령지농업연구소)
  • Published : 2007.02.28
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Abstract

The species in genus Saxifiraga distributed in circumpolar arctic are taxonomically difficult to study. RAPD analyses were performed to compare the genetic diversity of the 16 Saxifrages originated from the Norwegian Arctic Svalbard and Korea. The 12 accessions of URP primers were tested and 4 of which showed polymorphism were selected. Total 79 (44.8%) DNA bands were scored and analyzed by UPGMA cluster analysis. The results indicated that all of the 9 Saxifraga species from Svalbard showed high genetic diversity than those from Korea. The Similarity matrix and cluster analyses indicated that the Saxifraga species from Svalbard and Korea can be divided into two different subgroups. RAPDs of the Saxifraga species of Korea showed higher homologous patterns than those of Arctic Saxifrage. Among the Saxifraga species, we found that the morphological similarity reflects the genetic similarity. The geographic distance, clonal reproduction, and environmental condition may contribute the high level of genetic diversity between Saxifraga species from the two isolated regions.

북극지역에 분포하는 Saxifraga 속에 식물종들은 분류가 까다롭고 연구보고가 매우 적다. 북극권인 노르웨이령 Svalbard와 한국에서 수집된 16종의 Saxifraga 속 식물의 RAPD를 통하여 유전적 다형성을 비교하였다. 실험에 활용한 12 종류의 URP primer 중 4종의 primer를 이용하여 총 79개(44.8%)의 다형성을 가진 DNA 밴드를 선발하여 UPGMA 분석으로 유전적 다형성을 비교하였다. 그 결과 북극 Svalbard에서 수집된 9종의 Saxifraga 속 식물들이 한국에서 수집된 종들에 비해 유전적 다형성이 큰 것으로 확인되었다. 유사도 매트릭스를 활용한 분석 결과는 두 지역에 수집된 종들간에 비교적 뚜렷한 구룹을 형성하였으며, 한국 분포종의 RAPD는 북극 Svalbard 지역에 분포하는 종들에 비해 유사도가 높은 것으로 분석되었다. 본 연구를 통하여 우리는 두 지역에 분포하는 종들 간의 형태적 유사성이 유전적 유사성과 일치하고 있음을 확인하였다. 또한 분석 결과를 통하여 두 지역간의 지리적 격리, 집단내 영양 생식, 그리고 두 지역간의 환경조건의 차이가 높은 수준의 유전적 다형성을 형성하는 요인이 될 것으로 추론할 수 있었다.

Keywords

References

  1. Abbott, R.J., H.W. Chapman, R.M.M. Crawford and D.G. Forbes. 1995. Molecular diversity and derivations of populations of Silene acaulis and Saxifraga oppositifolia from the high Arctic and more southerly latitudes. Mol. Ecol. 4: 199-207 https://doi.org/10.1111/j.1365-294X.1995.tb00209.x
  2. Abbott, R.J., L.C. Smith, R.I. Milne, R.M. Crawford, K. Wolff and J. Balfour. 2000. Molecular analysis of plant migration and refugia in the Arctic. Science. 289: 1343-1346 https://doi.org/10.1126/science.289.5483.1343
  3. Alfonso, H.D.R. and J.B. Bamberg. 2000. RAPD markers efficiently distinguish heterogenous populations of wild potato (Solanum). Gen. Res. and Crop Evol. 47:115-121 https://doi.org/10.1023/A:1008725121904
  4. Bauert, M.R. 1993. Vivipary in polygonum viviparum: An adaptation to cold climate? Nord. J. Bot. 13: 473-480 https://doi.org/10.1111/j.1756-1051.1993.tb00085.x
  5. Brochmann, C., Q.Y. Xiang, S.J. Brunsfeld, D.E. Soltis and P.S. Soltis. 1998. Molecular evidence for polyploid origins in Saxifraga (Saxifragaceae): The narrow arctic endemic S. svalbardensis and its widespread allies Amer. J. Bot. 85: 135-143 https://doi.org/10.2307/2446562
  6. Buntjer, J.B., M. Otsen, I.J. Nijman, M.T.R. Kuiper and J.A. Lenstra. 2002. Phylogeny of bovine species based on AFLP fingerprinting. Heredity 88: 46-51 https://doi.org/10.1038/sj.hdy.6800007
  7. Chwedorzewska, K.J., B. Wojtun and O. Bednarek. 2005. Genetic and morphological variation of Saxifraga caespitosa L. from Spitsbergen (Svalbard): a preliminary study. Polar Biol. 28: 802-804 https://doi.org/10.1007/s00300-005-0023-3
  8. Congiu, L., M. Chicca, R. Cella, R. Rossi and G. Bernacchia. 2000. The use of random amplified polymorphic DNA (RAPD) markers to identify strawberry varieties: a forensic application. Mol. Ecol. 9: 229-232 https://doi.org/10.1046/j.1365-294x.2000.00811.x
  9. Crawford, R.M.M. and R.J. Abbott. 1994. Pre-adaptation of arctic plants to climate change. Bot. Acta. 107: 271-278 https://doi.org/10.1111/j.1438-8677.1994.tb00796.x
  10. Dubouzet, J.G., N. Murata and K. Shinoda. 1997. RAPD analysis of genetic relationships among Alstroemeria L. cultivar. Scientia Horticulturae 68: 181-189 https://doi.org/10.1016/S0304-4238(96)00966-1
  11. Dudash, M.R. 1990. Relative fitness of selfed and out-crossed progeny in a self-compatible, protandrous species, Sabatia angularis L. (Gentianaceae): a comparison in three environments. Evolution 44: 1129-1139 https://doi.org/10.2307/2409277
  12. Gabbrielsen, T.M., K. Bachmann, K.S. Jakobsen and C. Brochmann. 1997. Glacial survival doesn't matter: RAPD phylogeography of Nordic Saxifraga oppositifolia, Mol. Ecol. 6: 831-842 https://doi.org/10.1111/j.1365-294X.1997.tb00137.x
  13. Gabrielsen, T.M. and C. Brochmann. 1998. Sex after all: High levels of diversity detected in the Arctic clonal plants Saxifraga cernua using RAPD markers. Mol. Ecol. 7: 1701-1708 https://doi.org/10.1046/j.1365-294x.1998.00503.x
  14. Gugerli, F. 1998. Effect of elevation on sexual reproduction in alpine populations of Saxifraga oppositifolia (Saxifragaceae). Oecologia 114: 60-66 https://doi.org/10.1007/s004420050420
  15. Holderegger, R. and R.J. Abbott. 2003. Phylogeography of the Arctic- Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and ITS sequence variation. Amer. J. Bot. 90: 931- 936 https://doi.org/10.3732/ajb.90.6.931
  16. Hong, S.Y., T.T. Suh, K.S. Cho, Y.H. Han and S.Y. Ryu. 2001. Analysis genetic similarity of Saxifraga by randomly amplified polymorphic DNA. Kor. J. Hort. Sci. & Technol. 19 (Suppl. II): 94. (in Korean)
  17. Kapralov, M.V. 2004. Genotypic variation in populations of the clonal plant Saxifraga cernua in the central and peripheral regions of the species range, Russ. J. Ecol. 6: 413-416
  18. Kjolner S., S.M. Sastad, P. Taberlet and C. Brochmann. 2004. Amplified fragment length polymorphism versus random amplified polymorphic DNA markers: clonal diversity in Saxifraga cernua. Mol. Ecol. 13: 81-86 https://doi.org/10.1046/j.1365-294X.2003.02037.x
  19. Lande, R. and S. Shannon. 1996. The role of genetic variation in adaptation and population persistence in a changing environment. Evolution 50: 434-437 https://doi.org/10.2307/2410812
  20. Lee, K.E., Y.N. Song and Y.J. Park. 1985. Studies on certain growing aspects of wild Saxifraga fortunei var . glabrescens in Korea. Kor. J. Soc. Hort. Sci. 26: 51-58. (in Korean)
  21. Maynard, S.J. 1989. Evolutionary genetics. Oxford University Press, Oxford
  22. Miller, J., M. Litvak, S. Kelso and A. Vargo. 1994. Comparative reproductive biology of two alpine Primrose species. Arct. Alp. Res. 26: 297-303 https://doi.org/10.2307/1551942
  23. Molau, U. 1992. On the occurrence of sexual reproduction in Saxifraga cernua and S. foliolosa (Saxifragaceae), Nord. J. Bot. 12: 197-203 https://doi.org/10.1111/j.1756-1051.1992.tb01290.x
  24. Nei, M. 1972. Genetic distance between population. Amer. Nat. 106: 283-292 https://doi.org/10.1086/282771
  25. Parfitt, D.E. and M.L. Badenes. 1997. Phylogeny of the genus Pistacia as determined from analysis of the chloroplast genome. Proc. Natl. Acad. Sci. USA 94: 7987-7992
  26. Richards, A.J. 1986. Plant breeding systems. Allen & Unwin, London
  27. Ronning, O.I., 1996. The flora of Svalbard. Norwegian Polar Institute, Oslo. pp. 53-61
  28. Sneath, P.H.A. and R.R. Sokal. 1973. Numerical Taxonomy. Terrman, San Francisco
  29. Sokal, R.R. and C.D. Michener. 1958. A statistical method for evaluating systematic relationships. Univ. Kans. Sci. Bull. 38: 1409- 1438
  30. Vargas, P., B.G. Baldwin and L. Constance. 1998. Nuclear ribosomal DNA evidence for a western North American origin of Hwaiian South American species of Sanicula (Apiaceae). Proc. Natl. Acad. Sci. USA 95: 235-240
  31. Weeb, D. and R.J. Gronall. 1989. A manual of Saxifrages and their cultivation. Timber Press, Oregon
  32. 이영노. 1998. 한국식물도감. 교학사. 서울. pp. 281-284