Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Effective Identification of Rose Pollen Fertility using Staining Methods

장미화분 임성 확인을 위한 효율적인 염색법

  • Kim, Gi-Jun (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Hee-Ra (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Lee, Ja-Hyun (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Gi, Gwang-Yeon (Department of Horticulture, Jeonnam Agricultural Research and Extention Services) ;
  • Lee, Jeong-Hyun (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Han, Tae-Ho (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Choi, Jeong-Keun (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University)
  • 김기준 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 김희라 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 이자현 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 기광연 (전남농업기술원) ;
  • 이정현 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 한태호 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 최정근 (전남대학교 농업생명과학대학 식물생명공학부)
  • Published : 2007.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Efficient pollination needs abundant fertile pollen in rose breeding. This study was performed to find out efficient staining methods for the detection of fertile pollen. Aceto-carmine and Alexander's stain gave similar results in terms of percentage of normal pollen. Fluorochromatic reaction (FCR) showed the lowest normal pollen percentage because FCR stained only fertile pollen while others stained cytoplasm. Toluidine blue O (TB) showed similar percentage of normal pollen to Aceto-carmine and Alexander's, but could not clearly distinguish the clustered abnormal pollens. Alexander's stain was easy and simple, but difficult to distinguish fertile and infertile pollen. FCR showed only fertile pollen. Alexander's stain showed approximate fertility and FCR showed exact pollen fertility.

교배효율의 증대를 위해서는 정상화분의 확보가 선행되어야하며 화분의 활성을 파악할 필요가 있다. 본 연구에서는 화분의 활성을 확인하기 위해 여러 가지 염색액을 사용하여 광학현미경하에서 관찰하였다. Aceto-carmine과 Alexander's 염색액을 사용한 실험에서 비슷한 정상화분율을 얻을 수 있었고 FCR염색으로 관찰한 결과 살아있는 화분만 정확히 선별해 주기에 가장 낮은 정상화분율을 얻을 수 있었다. TB는 화분의 상태에 따라 달랐으나 Aceto-carmine과 Alexander's 염색액을 사용한 실험에서의 결과와 비슷한 양상을 보였다. 화분 관찰의 경험이 별로 없는 실험자도 쉽게 접근할 수 있는 염색액은 Alexander's 이었다. 정확한 화분의 활성을 조사하는 데에는 FCR이 필수이다. 형광현미경 장치 없이 정상과 비정상화분의 구별이 용이한 Alexander's 염색액으로 관찰한 자료를 바탕으로 살아있는 정상화분율의 예견이 가능하나 정확한 임성을 확인하려면 추가로 FCR test를 해야 한다.

Keywords

References

  1. Alexander, M.P. 1969. Differential staining of aborted and non-aborted pollen. Stain Technol. 44: 118-122
  2. Aylor, D.E. 2004. Survival of maize (Zea mays) pollen exposed in the atmosphere. Agri. Forest. Meteorogy 123: 125-133 https://doi.org/10.1016/j.agrformet.2003.12.007
  3. Cole, P. and B. Melton. 1986. Self- and cross-compatibility relationships among genotypes and between ploidy of the rose. J. Amer. Soc. Hort. Sci. 111: 122-125
  4. Feder, N. and T.P. O'Brien. 1968. Plant micro-technique; some principles and new methods. Am. J. Bot. 55: 123-142 https://doi.org/10.2307/2440500
  5. Fonseca, A.E. and M.E. Westgate. 2005. Relationship between desiccation and viability of maize pollen. Field Crops Research 94: 14-125 https://doi.org/10.1016/j.fcr.2004.11.006
  6. Heslop-Harrison, J. and Y. Heslop-Harrison. 1970. Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate. Stain Technol. 45: 115-120 https://doi.org/10.3109/10520297009085351
  7. Khosh-Khui, M., A. Bassiri and M. Niknejad. 1976. Effects of temperature and humidity on pollen viability of six rose species. Can. J. Plant Sci. 56: 517-523 https://doi.org/10.4141/cjps76-085
  8. Koncalova, M.N. 1975. Studies in rose pollen I. In vtro germination of pollen grains of Rosa hugonis. Preslia, Praha 47: 22-25
  9. Marcellan, O.N. and E.L. Camadro. 1996. The viability of asparagus pollen after storage at low temperatures. Sci. Hort. 67: 101-104 https://doi.org/10.1016/S0304-4238(96)00949-1
  10. Marchant, R., J.B. Power, M.R. Davey, J.M. Chartier-Hollis and P.T. Lynch. 1993. Cryopreservation of pollen from two rose cultivars. Euphytica 66: 235-241 https://doi.org/10.1007/BF00025309
  11. Ortiz, R., N. Vorsa, L.P. Bruederle and T. Laverty. 1999. Pollen viability in natural populations of tree north American dipoid species of blueberry (Vaccinium, section Cyanococcus). Sci. Hort. 80: 39-48 https://doi.org/10.1016/S0304-4238(98)00220-9
  12. Parantainen, A. and P. Pulkkinen. 2002. Pollen viability of scots pine (Pinus sylvestris) in different temperature conditions: high levels of variation among and within latitudes. Forest Eco. Manage. 167: 149- 160 https://doi.org/10.1016/S0378-1127(01)00722-8
  13. Rajasekharan, P.E. and S. Ganeshan. 1994. Freeze preservation of rose polllen in liquid nitrogen: Feasibility, viability and fertility status after long-term storage. J. Hort. Sci. 69: 565-569
  14. Vaknin, Y., D. Mills and A. Benzioni. 2003. Pollen production and pollen viability in male jojoba plants. Idustrial Crops Products 18: 117-123 https://doi.org/10.1016/S0926-6690(03)00038-4
  15. Visser, T., D.P. De Vries, G.W.H. Welles and J.A.M. Scheurink. 1977a. Hybrid tea-rose pollen. I. Germination and storage. Euphytica 26: 721-728 https://doi.org/10.1007/BF00021697
  16. Visser, T., D.P. De Vries, G.W.H. Welles and J.A.M. Scheurink. 1977b. Hybrid tea-rose pollen. II. Inheritance of pollen viability. Euphytica 26: 729-732 https://doi.org/10.1007/BF00021698
  17. Voyiatzi, C.I. 1995. An assessment of the in vitro germination capacity of pollen grains of five tea hybrid rose cultivars. Euphytica 83: 199- 204 https://doi.org/10.1007/BF01678130
  18. 한국농촌경제연구원. 2003. 장미품종 로열티 권리화 대응방안 연구. 농림부