식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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PCR Assay 이용 콩 종자에서 Xanthomonas axonopodis pv. glycines 검출 및 종자오염 조사

Detection of Xanthomonas axonopodis pv. glycines and Survey on Seed Contamination in Soybean Seeds Using PCR Assay

  • 홍성준 (작물과학원 영남농업연구소 식물환경과) ;
  • 홍연규 (농업과학기술원 농업해충과) ;
  • 이봉춘 (작물과학원 영남농업연구소 식물환경과) ;
  • 임미정 (작물과학원 영남농업연구소 식물환경과) ;
  • 윤영남 (작물과학원 영남농업연구소 식물환경과) ;
  • 황재복 (작물과학원 영남농업연구소 식물환경과) ;
  • 송석보 (작물과학원 영남농업연구소 식물환경과) ;
  • 박성태 (작물과학원 영남농업연구소 식물환경과)
  • Hong, Sung-Jun (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Hong, Yeon-Kyu (Applied Entomology Division, National Institute of Agricultural Science and Technology) ;
  • Lee, Bong-Choon (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Lim, Mi-Jung (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Yoon, Young-Nam (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Hwang, Jae-Bok (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Song, Seok-Bo (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Park, Sung-Tae (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA)
  • 발행 : 2007.12.01
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초록

Xanthomonas axonopodis pv. glycines에 의해 발병되는 콩 불마름병은 한국에서 콩에 가장 많이 발생하는 중요한 세균성 병해 중 하나이다. 본 연구에서는 Xanthomonas axonopodis pv. glycines를 종자에서 검출하기 위해 PCR기법을 이용하였으며, 한국의 36개 주요 콩 품종의 종자 오염을 조사하였다. 그리고 병원균 검출과 동정을 위한 PCR assay와 dilution plating assay를 비교하였다. PCR assay를 이용하여 인공접종에 의한 이병종자와 자연감염된 이병종자로부터 병원균 검출을 확인하였다. PCR assay를 통한 이런 결과는 dilution plating assay와 비슷한 결과를 보여 주었으며 종자에서 병원균을 검출하는 다른 전통적인 방법보다 더 효과적인 방법으로 증명되었다. 36개 주요 콩 품종의 X. axonopodis pv. glycines에 의한 종자 전염을 확인한 결과 풍산나물콩, 만리콩, 태광콩, 대망콩, 아주까리콩에서 병원균이 검출되었다. 그러므로 PCR assay는 콩 종자에서 신속하고, 민감하게 X. axonopodis pv. glycines 특이적으로 검출할 수 있는 효과적인 방법으로 활용될 수 있을 것이다.

Xanthomonas axonopodis pv. glycines is the causal agent of bacterial pustule of soybean(Glycine max. (L.) Merr), which is one of the most prevalent bacterial diseases in Korea. In this study, Polymerase Chain Reaction (PCR) assay was applied to detect Xanthomonas axonopodis pv. glycines and to survey on seed contamination in 36 soybean cultivars of Korea. And we have to compare PCR assay with dilution-plating assay of detection and identification. We confirmed detection of pathogen from artificial infected seeds and natural Infected seeds using PCR assay. This assay gave results similar to a seed-wash dilution plating assay and proved more effective than classical methods. Results of survey on seed contamination by X. axonopodis pv. glycines from 36 cultivar seeds showed that the pathogen was detected from Pungsan-namulkong, Mallikong, Taekwangkong, Daemangkong, Ajukkarikong using PCR assay. Therefore, The PCR assay provides a sensitive, rapid tool for the specific detection of X. axonopodis pv. glycines in soybean seeds.

키워드

참고문헌

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피인용 문헌

  1. One-step Multiplex RT-PCR Method for Simultaneous Detection of Seed Transmissible Bacterium and Virus Occurring on Brassicaceae Crop Seeds vol.17, pp.1, 2011, https://doi.org/10.5423/RPD.2011.17.1.052
  2. Multiplex PCR Assay for the Simultaneous Detection of Major Pathogenic Bacteria in Soybean vol.58, pp.2, 2013, https://doi.org/10.7740/kjcs.2013.58.2.142