The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Study on Environmental Risk Assessment for Potential Effect of Genetically Modified Nicotiana benthamiana Expressing ZGMMV Coat Protein Gene

  • Kim, Tae-Sung (LMO Environmental Risk Evaluation Team, Ecosystem Disturbance Assessment Division, Nature and Ecology Research Department, National Institute of Environmental Research) ;
  • Yu, Min-Su (LMO Environmental Risk Evaluation Team, Ecosystem Disturbance Assessment Division, Nature and Ecology Research Department, National Institute of Environmental Research) ;
  • Koh, Kong-Suk (LMO Environmental Risk Evaluation Team, Ecosystem Disturbance Assessment Division, Nature and Ecology Research Department, National Institute of Environmental Research) ;
  • Oh, Kyoung-Hee (LMO Environmental Risk Evaluation Team, Ecosystem Disturbance Assessment Division, Nature and Ecology Research Department, National Institute of Environmental Research) ;
  • Ahn, Hong-Il (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University) ;
  • Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University)
  • Published : 2006.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Transgenic Nicotiana benthamiana plants harboring the coat protein(CP) gene of Zucchini green mottle mosaic virus(ZGMMV) were chosen as a model host for the environmental risk assessment of genetically modified plants with virus resistance. This study was focused on whether new virus type may arise during serial inoculation of one point CP mutant of ZGMMV on the transgenic plants. In vitro transcripts derived from the non-functional CP mutant were inoculated onto the virus-tolerant and -susceptible transgenic N. benthamiana plants. Any notable viral symptoms that could arise on the inoculated transgenic host plants were not detected, even though the inoculation experiment was repeated a total of ten times. This result suggests that potential risk associated with the CP-expressiing transgenic plants may not be significant. However, cautions must be taken as it does not guarantee environmental safety of these CP-mediated virus-resistant plants, considering the limited number of the transgenic plants tested in this study. Further study at a larger scale is needed to evaluate the environmental risk that might be associated with the CP-mediated virus resistant plant.

Keywords

References

  1. Aaziz, R. and Tepfer, M. 1999a. Recombination between genomic RNAs of two cucumoviruses under conditions of minimal selection pressure. Virology 263:282-289 https://doi.org/10.1006/viro.1999.9973
  2. Aaziz, R. and Tepfer, M. 1999b. Recombination in RNA viruses and in virus-resistant transgenic plants. J. Gen. Virol. 80:1339-1346 https://doi.org/10.1099/0022-1317-80-6-1339
  3. Bau, H. J., Cheng, Y. H., Yu, T. A., Yang, J. S., Liou, P. C., Hsiao, C. H., Lin, C. Y. and Yeh, S. D. 2004. Field evaluation of transgenic papaya lines carrying the coat protein gene of Papaya ringspot virus in Taiwan. Plant Dis. 88:594-599 https://doi.org/10.1094/PDIS.2004.88.6.594
  4. Borja, M., Rubio, T., Scholthof, H. B. and Jackson, A. O. 1999. Restoration of wild-type virus by double recombination of tombusvirus mutants with a host transgene. Mol. PlantMicrobe Interact. 12:153-162 https://doi.org/10.1094/MPMI.1999.12.2.153
  5. de Zoeten, G. A. 1991. Risk assessment: Do we let history repeat itself? Phytopathology 81:585
  6. Falk, B. W., Passmore, B. K., Watson, M. T. and Chin, L-S. 1995. The specificity and significance of heterologous encapsidation of virus and virus-like RNA's. In: Biotechnology and Plant Protection. Viral pathogenesis and disease resistance, ed. by S-D. Kang and D. D. Bill, pp. 391-451. World Scientific, Singapore
  7. Fuchs, M. Klas, F. E., McFerson, J. R. and Gonsalves. D. 1998. Transgenic melon and squash expressing coat protein genes of aphid-borne viruses do not assist the spread of an aphid nontransmissible strain of cucumber mosaic virus in the field. Transgenic Res. 7:449-462 https://doi.org/10.1023/A:1008828500686
  8. Fuchs, M., Gal-On, A., Raccah, B. and Gonsalves, D. 1999. Epidemiology of an aphid nontransmissible potyvirus in fields of nontransgenic and coat protein transgenic squash. Transgenic Res. 8:429-439 https://doi.org/10.1023/A:1008935426211
  9. Hammond, J., Lecoq, H. and Raccah, B. 1999. Epidemiological risks from mixed virus infections and transgenic plants expressing viral genes. Adv. Virus Res. 54:189-314 https://doi.org/10.1016/S0065-3527(08)60368-1
  10. Ito, W., Ishiguro, H. and Kurosawa, Y. 1991. A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction. Gene 102:37-37
  11. Lai, M. M. C. 1992. RNA recombination in animal and plant viruses. Microbiol. Rev. 56:61-79
  12. Li, H. G. and Roossinck, M. J. 2004. Genetic bottlenecks reduced population variation in an experimental RNA virus population. J. Virol. 78:10582-10587 https://doi.org/10.1128/JVI.78.19.10582-10587.2004
  13. Matthews, R. E. F. 1991. Plant Virology, Acadamic Press, New York, 835pp
  14. Kim, M. J., Choi, S. H., Kim, T., Park, M. H., Lim, H. R., Oh, K. H., Kim, T., Lee, M. H. and Ryu, K. H. 2004. Virus resistant and susceptible transgenic Nicotiana benthamiana plants expressing coat protein gene of Zucchini green mottle mosaic virus for LMO safety assessment. Plant Pathol. J. 20:206-211 https://doi.org/10.5423/PPJ.2004.20.3.206
  15. Nagy, P. D. and Simon, A. E. 1997. New insight into the mechanisms of RNA recombination. Virology 235:1-9 https://doi.org/10.1006/viro.1997.8681
  16. OECD, 1996. Consensus document on general information concerning the biosafety of crop plants made virus resistant through coat protein gene-mediated protection, OECD/ GD(96) 162:20-29
  17. Powell Abel, P., Nelson, R. S., De, B., Hoffman, N., Rogers, S. G., Fraley, R. T. and Beachy, R. N. 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738-743 https://doi.org/10.1126/science.3457472
  18. Ryu, K. H., Min, B. E., Choi, G. S., Choi, S. H., Kwon, S. B., Noh, G. M., Yoon, J. Y., Choi, Y. M., Jang, S. H., Lee, G. P., Cho, K. H. and Park, W. M. 2000. Zucchini green mottle mosaic virus is a new tobamovirus: comparison of its coat protein gene with that of kyuri green mottle mosaic virus. Arch. Virol. 145:2325-2333 https://doi.org/10.1007/s007050070023
  19. Sambrook, J., Fritsch, E. F. and Maniatis, T. 2001, Molecular cloning: a laboratory manual, 2nd ed., Cold Springer Harbor Laboratory Press, New York
  20. Sacristan, S. Malpica, J. M., Fraile, A. and Garcia-Arenal, F. 2003. Estimation of population bottlenecks during systemic movement of tobacco mosaic virus in tobacco plants, J. Virol. 77:9906-9911 https://doi.org/10.1128/JVI.77.18.9906-9911.2003
  21. Thomas, P. E., Hassan, S., Kaniewski, W. K., Lawson, E. C. and Zalewski, J. C. 1998. A search for evidence of virus/transgene interactions in potatoes transformed with the potato leafroll virus replicase and coat protein genes. Mol. Breed. 4:407-417 https://doi.org/10.1023/A:1009693927819
  22. Wintermantel, W. M. and Schoelz, J. E. 1996. Isolation of recombinant viruses between cauliflower mosaic virus and a viral gene in transgenic plants under conditions of moderate selection pressure. Virology 223:156-164 https://doi.org/10.1006/viro.1996.0464