Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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DNA Separation Using Cellulose Derivatives and PEO by PDMS Microchip

  • Published : 2006.04.20
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Abstract

Poly(dimethyl siloxane) (PDMS) has been employed as a microchip material for DNA separation in microfluidic condition. Different sieving molecules such as cellulose derivatives having glucose building block (methyl cellulose (MC), hydroxyethyl cellulose (HEC), and hydroxypropyl methyl cellulose (HPMC)) and polyethylene oxide (PEO) having linear (ring-opened ethylene oxide) unit were used and their performance was compared in terms of separation efficiency and resolution. In general, PEO showed better separation performance than cellulose derivatives probably due to the nature of linear shape polymer conformation. It was possible to perform at least 15 consecutive running with 1.2% PEO at the electric field strength around 200 V/cm. Fast analysis of the standard $\Phi$X 174 RF DNA/Hae III (less than 130s) was obtained with the number of the theoretical plate around 250,000/m. Our PMDS microchip was applied to the measurement of CAG repeat number, which is related to male infertile disease.

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References

  1. Ken-Ichiro, Y.; Masataka, Y.; Kouji, C.; Mikihiko, H.; Satoshi, K. Urology 1999, 54(6), 1078 https://doi.org/10.1016/S0090-4295(99)00312-X
  2. Ruiz-Martinez, M. C.; Berka, J.; Belenkii, A.; Foret, F.; Miller, A. W.; Karger, B. L. Anal. Chem. 1993, 65, 2851 https://doi.org/10.1021/ac00068a023
  3. Srinivasan, K.; Pohl, G.; Avdalovic, N. Anal. Chem. 1997, 69, 2798 https://doi.org/10.1021/ac970041m
  4. Mathies, R. A.; Huang, X. C. Nature 1992, 359, 167 https://doi.org/10.1038/359167a0
  5. Liu, S.; Guttman, A. TrAC Trends in Analytical Chemistry 2004, 23, 422 https://doi.org/10.1016/S0165-9936(04)00602-8
  6. Proceedings of $\mu$TAS Symposium 2000; Berg, A. van. Den.; Olthuis, W.; Berveld, P., Eds.; Kluwer Academic Publishers: Dordrecht, The Netherlands, 2000
  7. Soper, S. A.; Ford, S. M.; Qi, S.; McCarley, R. L.; Kelly, K.; Murphy, M. C. Anal. Chem. 2000, 72, A-Pages
  8. Kang, S. H.; Jeong, S. J.; Kim, D. K.; He, Y.; Yeung, E. S. Bull. Korean Chem. Soc. 2005, 26(2), 315 https://doi.org/10.5012/bkcs.2005.26.2.315
  9. Jang, S. M.; Cho, K. C.; Chae, J. S.; Kang, S. H. Bull. Korean Chem. Soc. 2004, 25(5), 757 https://doi.org/10.5012/bkcs.2004.25.5.757
  10. Martynova, L.; Locascio, L. E.; Gaitan, M.; Kramer, G. W.; Christensen, R. G.; MacCrehan, W. A. Anal. Chem. 1997, 69, 4783 https://doi.org/10.1021/ac970558y
  11. Martin, R. S.; Gawron, A. J.; Lunte, S. M.; Henry, C. S. Anal. Chem. 2000, 72, 3196 https://doi.org/10.1021/ac000160t
  12. Roberts, M. A.; Rossier, J. S.; Bercier, P.; Girault, H. Anal. Chem. 1997, 69, 2035 https://doi.org/10.1021/ac961038q
  13. Clarson, S. J.; Semlyen, J. A. Siloxane Polymers; Englewood Cliffs, NJ: Prentice-Hall, 1993
  14. Dow Corning Corp., Midland, MI
  15. Baltussen, E.; Sandra, P.; David, F.; Janssen, H. G.; Cramers, C. Anal. Chem. 1999, 71, 5213 https://doi.org/10.1021/ac990313g
  16. Thomas, C. D.; Jacobson, S. C.; Ramsey, J. M. Anal. Chem. 2004, 76, 6053 https://doi.org/10.1021/ac035475y
  17. Wang, B.; Abdulali-Kanji, Z.; Dodwell, E.; Horton, J. H.; Oleschuk, R. D. Electrophoresis 2003, 24, 1442 https://doi.org/10.1002/elps.200390186
  18. Duffy, D. C.; McDonald, J. C.; Schueller, O. J. A.; Whitesides, G. M. Anal. Chem. 1998, 70, 4974 https://doi.org/10.1021/ac980656z
  19. Ren, X.; Bachman, M.; Sims, C. E.; Li, G. P.; Allbritton, N. L. J. Chromatogr. B 2001, 762, 117 https://doi.org/10.1016/S0378-4347(01)00327-9
  20. Annelise, E. B.; Wade, M. S.; Harvey, W. B. Electrophoresis 1995, 16, 64 https://doi.org/10.1002/elps.1150160113
  21. Wang, S. C.; Perso, C. E.; Morris, M. D. Anal. Chem. 2000, 72, 1704 https://doi.org/10.1021/ac9909148
  22. La, S. A. R.; Wilson, E. M.; Lubahn, D. B.; Harding, A. E.; Fischbeck, K. H. Nature 1993, 352, 77 https://doi.org/10.1038/352077a0
  23. Campzano, V.; Montermini, L.; Molto, M. D.; Pianese, L.; Cosssee, M.; Cavalcant, F. Science 1996, 271, 1423 https://doi.org/10.1126/science.271.5254.1423
  24. Jennifer, L. H.; Donald, H. A.; Xu, J.-F.; Edward Highsmith, W. Jr. Electrophoresis 2004, 25, 1852 https://doi.org/10.1002/elps.200305811

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