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http://dx.doi.org/10.5012/bkcs.2006.27.4.519

DNA Separation Using Cellulose Derivatives and PEO by PDMS Microchip  

Kang, Chung-mu (Division of Natural Science, Kyungnam University)
Back, Seung-Kwon (Division of Natural Science, Kyungnam University)
Song, In-gul (Division of Natural Science, Kyungnam University)
Choi, Byung-ok (College of Medicine, EWHA Womans University)
Chang, Jun-keun (Digital Bio Technology, Rm. 511, SKC Central Research Institute)
Cho, Keun-chang (Digital Bio Technology, Rm. 511, SKC Central Research Institute)
Kim, Yong-seong (Division of Natural Science, Kyungnam University)
Publication Information
Bulletin of the Korean Chemical Society / v.27, no.4, 2006 , pp. 519-523 More about this Journal
Abstract
Poly(dimethyl siloxane) (PDMS) has been employed as a microchip material for DNA separation in microfluidic condition. Different sieving molecules such as cellulose derivatives having glucose building block (methyl cellulose (MC), hydroxyethyl cellulose (HEC), and hydroxypropyl methyl cellulose (HPMC)) and polyethylene oxide (PEO) having linear (ring-opened ethylene oxide) unit were used and their performance was compared in terms of separation efficiency and resolution. In general, PEO showed better separation performance than cellulose derivatives probably due to the nature of linear shape polymer conformation. It was possible to perform at least 15 consecutive running with 1.2% PEO at the electric field strength around 200 V/cm. Fast analysis of the standard $\Phi$X 174 RF DNA/Hae III (less than 130s) was obtained with the number of the theoretical plate around 250,000/m. Our PMDS microchip was applied to the measurement of CAG repeat number, which is related to male infertile disease.
Keywords
PDMS; Microchip; DNA; PEO; Infertile;
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