Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
권호 표지

Isolation and Identification of Low Molecular Weight Compounds Produced by Bacillus subtilis HJ927 and Their Biocontrol Effect on the Late Blight of Pepper (Capsicum annuum L.)

Bacillus subtilis HJ927에 의해 생성된 화합물의 분리, 동정 및 고추(Capsicum annum L.) 역병방제 효과

  • Lee, Hyun-Jin (Glucosamin Saccharide Material Laboratory(NRL), Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Park, Keun-Hyung (Glucosamin Saccharide Material Laboratory(NRL), Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Shim, Jae-Han (Glucosamin Saccharide Material Laboratory(NRL), Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Park, Ro-Dong (Glucosamin Saccharide Material Laboratory(NRL), Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Kim, Yong-Woong (Glucosamin Saccharide Material Laboratory(NRL), Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Hwang-Bo, Hoon (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, APSRC, Chonnam National University) ;
  • Cho, Jeung-Yong (Glucosamin Saccharide Material Laboratory(NRL), Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Kim, Young-Cheol (Division of Applied Plant Science, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Kil-Yong (Glucosamin Saccharide Material Laboratory(NRL), Division of Applied Bioscience and Biotechnology, Chonnam National University)
  • 이현진 (전남대학교 생물환경과학부) ;
  • 박근형 (전남대학교 생물환경과학부) ;
  • 심재한 (전남대학교 생물환경과학부) ;
  • 박노동 (전남대학교 생물환경과학부) ;
  • 김용웅 (전남대학교 생물환경과학부) ;
  • 황보훈 (전남대학교 응용생물공학부) ;
  • 조정용 (전남대학교 생물환경과학부) ;
  • 김영철 (전남대학교 응용식물학부) ;
  • 김길용 (전남대학교 생물환경과학부)
  • Received : 2004.11.08
  • Accepted : 2004.12.14
  • Published : 2005.02.28
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Abstract

A soil bacterium, Bacillus subtilis HJ927, exhibiting strong antagonistic property against pathogenic fungi was isolated from pepper fields infested with Phytophthora capsici. Pepper plants inoculated with P. capsici revealed severe root mortality while plants co-inoculated with B. subtilis HJ927 and P. capsici showed drastically reduced root mortality. Low molecular weight substances released by B. subtilis HJ927 mediated the plant protective effect. The anti-fungal compounds released by B. subtilis HJ927 were identified as 3-methylbutyric acid, 2-methylbutyric acid, and methyl 2-hydroxy, 3-phenylpropanoate by high-performance liquid chromatography and gas chromatography-mass spectrometry. In addition to these compounds, B. subtilis HJ927 also produced ${\beta}$-1,3-glucanase, a hydrolytic enzyme implicated in antifungal activity.

병원성 곰팡이에 대해 강한 길항성을 보이는 토양미생물 Bacillus subtilis HJ927을 Phytophthora capsici에 감염된 고추밭으로부터 분리해냈다. B. subtilis HJ927을 P. capsici와 함께 고추에 처리한 결과 P. capsici만을 접종한 처리구에 비해 크게 식물을 보호하는 것을 root mortality 측정결과 확인하였다. B. subtilis HJ927는 항곰팡이성 물질로 3-methylbutyric acid, 2-methylbutyric acid, 그리고 methyl 2-hydroxy, 3-phenyipropanoate를 분비해 내는 것을 HPLE와 GC-MS를 통해 분리 동정하였다. 또한 B. subtilis HJ927는 가수분해효소인 ${\beta}$-1,3-glucanase를 분비해 냄으로서 위 화합물과 함께 식물을 보호하는 것으로 분석되었다.

Keywords

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