Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Transfected HepG2 Cells for Evaluation of Catechin Effects on Alcohol-Induced CYP2E1 Cytotoxicity

  • LEE YOO-HYUN (School of Life Sciences & Biotechnology, Korea University) ;
  • HO JIN-NYOUNG (School of Life Sciences & Biotechnology, Korea University) ;
  • DONG MI-SOOK (School of Life Sciences & Biotechnology, Korea University) ;
  • PARK CHANG-HWAN (College of Medicine, Hanyang University) ;
  • KIM HYE-KYUNG (Department of Food and Biotechnology, Hanseo University) ;
  • HONG BUMSHIK (School of Life Sciences & Biotechnology, Korea University) ;
  • SHIN DONG-HOON (School of Life Sciences & Biotechnology, Korea University) ;
  • CHO HONG-YON (School of Life Sciences & Biotechnology, Korea University)
  • Published : 2005.12.01
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Abstract

To evaluate the toxicological properties of human cytochrome P450 2E1 (CYP2E1) induced by ethanol and possible protective effects of various green tea catechins on alcohol-induced toxicity, transfected HepG2 cells that stably and constitutively express human CYP2E1 were established using the recombinant retroviral expression vector. Exposure of the CYP2E1-expressing HepG2 cells to high concentration of ethanol (200 mM) for 5 days resulted in a more than $50\%$ increase of cytotoxicity, assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction, lactate dehydrogenase (LDH) release, and reactive oxygen species (ROS) production, and loss of normal morphology, in comparison with HepG2 cells containing control vector. Treatment of the cells with various catechins increased cell viability by more than 2-fold. (-)-Epicatechin gallate and(-)-catechin gallate at the lowest concentration ($5\;{\mu}M$) attenuated cell death induced CYP2E1 by $60-65\%$. Therefore, the results showed that the catechins, including epimerized catechins, have strong protective effects against alcohol-induced CYP2E1 toxicity, and it is correlated with antioxidant effect.

Keywords

References

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