Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immobilization of Penicillium citrinum by Entrapping Cells in Calcium Alginate for the Production of Neo-Fructooligosaccharides

  • Lim, Jung-Soo (Department of Chemical and Biological Engineering, Korea University) ;
  • Park, Seung-Won (Food Ingredient Division, CJ Foods R & D, CJ Corp.,) ;
  • Lee, Jin-Won (Department of Chemical and Biomolecular Enginnering, Sogang University) ;
  • Oh, Kyeong-Keon (Department of Industrial Chemistry, Dankook University) ;
  • Kim, Seung-Wook (Department of Chemical and Biological Engineering, Korea University)
  • Published : 2005.12.01
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Abstract

This work describes neo-fructooligosaccharides (neo-FOSs) production using the immobilized mycelia of Penicillium citrinum. Some critical factors were evaluated to optimize maximal production of neo-FOS. Optimal alginate and cell concentrations were determined to be $1.96\%$ alginate and $7.17\%$ cell, respectively, by statistical analysis. The optimal concentration of $CaCl_{2}$, which is related to bead stability, was determined to be 2 M. It was possible to increase the neo-FOS production by adding 15 units of glucose oxidase to the batch reaction. By co-immobilizing cells and glucose oxidase, neoFOS productivity increased $123\%$ compared with the whole-cell immobilization process. Based on the results above, a co-immobilization technique was developed and it can be utilized for large-scale production.

Keywords

References

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