Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of Serum-Free Media for Primary Culture of Human Articular Chondrocytes

  • CHOI YONG SOO (Department of Biological Engineering, Inha University) ;
  • LIM SANG MIN (Department of Biological Engineering, Inha University) ;
  • LEE CHANG WOO (Good Shepherd Hospital) ;
  • KIM DONG-IL (Department of Biological Engineering, Inha University)
  • Published : 2005.12.01
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Abstract

Human articular chondrocytes (HAC) were cultivated as a monolayer in a serum-free medium for primary culture (SFM-P). An optimized SFM-P provides $95\%$ proliferation rate of that obtainable from primary and secondary chondrocyte cultures grown in a control medium with serum. The gradual decrease in the amounts of synthesized glycosaminoglycan and type II collagen was improved by coating the culture dishes with type IV collagen and fibronectin. A significant improvement in the expression of type II collagen and aggrecan mRNA could be achieved. In addition, the monolayer cultures showed better synthesis of the extracellular matrices than alginate-bead cultures in SFM-P.

Keywords

References

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