Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Oxidant Activities in Human Dermal Microvascular Endothelial Cells Infected with Orientia tsutsugamushi

Orientia tsutsugamushi에 감염된 인간 피부 미세혈관 내피세포의 산화 활성

  • Koh Young-Sang (Department of Microbiology, College of Medicine, Cheju National University)
  • 고영상 (제주대학교 의과대학 미생물학교실)
  • Published : 2005.09.01
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Abstract

Transcripts levels of superoxide dismutases increase slightly following infection of human dermal microvascular endothelial cells(HMEC-1) by the obligae intracellular bacterium Orientia tsutsugamushi, the causative agent of scrub typhus. In addition, fluorescence-activated cell sorter analysis demonstrates significant intracellular peroxide activity in infected cells within 5 hr after exposure to O. tsutsugamushi. Furthermore, infected cells experienced a significant depletion of glutathiones. These results support hypothesis that cells infected by this intracellular bacterium experience oxidant-mediated injury.

Scrub typhus의 원인병원체인 Orientia tsutsugamushi에 감염된 인간 피부 미세혈관 내피세포(HMEC-1)에서 superoxide dismutase 유전자의 발현이 증가하였다. O. tsutsugamushi 접촉 후 5 시간 이내에 감염된 세포에서 세포내 peroxide 존재를 유세포 분석을 통하여 관찰할 수 있었다. 또한 감염된 세포는 세포 내 glutathione이 심하게 고갈됨을 관찰하였다. 이 결과들은 이 절대세포내 기생세균에 감염된 숙주세포가 산화-매개 손상 받음을 시사한다.

Keywords

References

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