Browse > Article

Oxidant Activities in Human Dermal Microvascular Endothelial Cells Infected with Orientia tsutsugamushi  

Koh Young-Sang (Department of Microbiology, College of Medicine, Cheju National University)
Publication Information
Korean Journal of Microbiology / v.41, no.3, 2005 , pp. 232-235 More about this Journal
Abstract
Transcripts levels of superoxide dismutases increase slightly following infection of human dermal microvascular endothelial cells(HMEC-1) by the obligae intracellular bacterium Orientia tsutsugamushi, the causative agent of scrub typhus. In addition, fluorescence-activated cell sorter analysis demonstrates significant intracellular peroxide activity in infected cells within 5 hr after exposure to O. tsutsugamushi. Furthermore, infected cells experienced a significant depletion of glutathiones. These results support hypothesis that cells infected by this intracellular bacterium experience oxidant-mediated injury.
Keywords
HMEC-1; intracellular peroxide; Orientia tsutsugamushi; oxidativ injury;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ades, E.W., F.J. Candal, R.A. Swerlick, V.G. George, S. Summers, D.C. Bosse, and T.J. Lawley. 1992. HMEC-1: establishment of an immortalized human microvascular endothelial cell line. J. Investig. Dermatol. 99, 683-690   DOI   ScienceOn
2 Chi, W.C., J.J. Huang, J.M. Sung, R.R. Lan, W.C. Ko, and F.F. Chen. 1997. Scrub typhus associated with multiorgan failure: a case report. Scand. J. Infect. Dis. 29, 634-635   DOI   ScienceOn
3 Freeman B.A. and J.D. Crapo. 1982. Biology of disease: free radicals and tissue injury. Lab Invest. 47, 412-426   PUBMED
4 Griffith OW. 1980. Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem. 106, 207-212   DOI   PUBMED   ScienceOn
5 Ng, F.K., S.C. Oaks, Jr., M. Lee, M.G. Groves, and G.E. Lewis, Jr. 1985. A scanning and transmission electron microscopic examination of Rickettsia tsutsugamushi-infected human endothelial, MRC-5, and L-929 cells. Jpn. J. Med. Sci. Biol. 38, 125-139   DOI   PUBMED
6 Rikihisa, Y. and S. Ito. 1979. Intracellular localization of Rickettsia tsutsugamushi in polymorphonuclear leukocytes. J. Exp. Med.150, 703-708   DOI   ScienceOn
7 Silverman, D.J. and L.A. Santucci. 1988. Potential for free radical-induced lipid peroxidation as a cause of endothelial cell injury in Rocky Mountain spotted fever. Infect. Immun. 56, 3110-3115   PUBMED
8 Yotsukura, M., N. Aoki, N. Fukuzumi, and K. Ishikawa. 1991. Review of a case of tsutsugamushi disease showing myocarditis and confirmation of Rickettsia by endomyocardial biopsy. Jpn. Circ. J. 55, 149-153   DOI   PUBMED   ScienceOn
9 Cho, N.H., S.Y. Seong, M.S. Huh, T.H. Han, Y.S. Koh, M.S. Choi, and I.S. Kim. 2000. Expression of chemokine genes in murine macrophages infected with Orientia tsutsugamushi. Infect. Immun. 68, 594-602   DOI   ScienceOn
10 Allen, A.C. and S. Spitz. 1945. A comparative study of the pathology of scrub typhus (tsutsugamushi disease) and other rickettsial disease. Am. J. Pathol. 21, 603-681   PUBMED
11 Chang, W.H., J.S. Kang, W.K. Lee, M.S. Choi, and J.H. Lee. 1990. Serological classification by monoclonal antibodies of Rickettsia tsutsugamushi isolated in Korea. J. Clin. Microbiol. 28, 685-688   PUBMED
12 Leving, D.H. 1946. Pathologic study of thirty-one cases of scrub typhus fever with special reference to the cardiovascular system. Am. Heart J. 31, 314-328   DOI   ScienceOn
13 Carter, W.O., P.K. Narayanan, and J.P. Robinson. 1993. Intracellular hydrogen peroxide and superoxide anion detection in endothelial cells. J. Leukocyte Biol. 55, 253-258
14 Smuckler, E.A. 1976. Alterations produced in the endoplasmic reticulum by carbon tetrachloride. Panminerva Med. 18, 292-309   PUBMED
15 Pongponratn, E., Y. Maneerat, U. Chaisri, P. Wilairatana, B. Punpoowong, P. Viriyavejakul, and M. Riganti. 1998. Electron-microscopic examination of Rickettsia tsutsugamushi-infected human liver. Trop. Med. Int. Health 3, 242-248   DOI   ScienceOn
16 Kim, M.K., S.H. Kee, K.A. Cho, M.H. Chung, B.U. Lim, W.H. Chang, and J.S. Kang. 1999. Apoptosis of endothelial cell line ECV304 persistently infected with Orientia tsutsugamushi. Microbiol. Immunol. 43, 751-757   DOI   PUBMED