Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Apoptotic Effect of Luteolin Isolated from Scutellaria barbata

반지련으로 부터 분리한 luteolin의 세포고사효과

  • Lee, Eun-Ok (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kim, Jin-Hyung (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Park, Young-Doo (College of Life Science, Kyunghee University) ;
  • Kim, Sung-Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
  • 이은옥 (경희대학교 동서의학대학원 종양학실험실) ;
  • 김진형 (경희대학교 동서의학대학원 종양학실험실) ;
  • 안규석 (경희대학교 한의과대학 병리학교실) ;
  • 박영두 (경희대학교 생명과학부) ;
  • 김성훈 (경희대학교 동서의학대학원 종양학실험실)
  • Published : 2005.08.01
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Abstract

We previously demonstrated that the methylene chloride fraction of Scutellaria barbata suppessed human leukemic U937 cell proliferation by inducing apoptosis. In the present study, we have isolated luteolin from Scutellaria barbata and evaluated its apoptotic mechanism in Lewis lung carcinoma cells. Luteolin inhibited the proliferation of Lewis lung carcinoma cells in a concentration-dependent manner. Luteolin effectively increased the portion of $sub-G_1$ DNA content (apoptotic portion) and apoptotic Annexin-V positive cells in a concentration-dependent manner by FACS analysis. Caspase 9 and caspase 3 were activated and PARP was effectively cleaved by luteolin. It also increased the ratio of Bax to Bcl-2 through the decrease of Bcl-2 expression by Western blotting and reduced mitochondrial membrane potential following TMRE staining. These results suggest that luteolin can induce apoptosis through the mitochondrial mediated pathway.

Keywords

References

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