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Apoptotic Effect of Luteolin Isolated from Scutellaria barbata  

Lee, Eun-Ok (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
Kim, Jin-Hyung (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Park, Young-Doo (College of Life Science, Kyunghee University)
Kim, Sung-Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.19, no.4, 2005 , pp. 955-959 More about this Journal
Abstract
We previously demonstrated that the methylene chloride fraction of Scutellaria barbata suppessed human leukemic U937 cell proliferation by inducing apoptosis. In the present study, we have isolated luteolin from Scutellaria barbata and evaluated its apoptotic mechanism in Lewis lung carcinoma cells. Luteolin inhibited the proliferation of Lewis lung carcinoma cells in a concentration-dependent manner. Luteolin effectively increased the portion of $sub-G_1$ DNA content (apoptotic portion) and apoptotic Annexin-V positive cells in a concentration-dependent manner by FACS analysis. Caspase 9 and caspase 3 were activated and PARP was effectively cleaved by luteolin. It also increased the ratio of Bax to Bcl-2 through the decrease of Bcl-2 expression by Western blotting and reduced mitochondrial membrane potential following TMRE staining. These results suggest that luteolin can induce apoptosis through the mitochondrial mediated pathway.
Keywords
Scurellaria barbata; luteolin; apoptosis; Annexin V; bcl-2; mitochondrial membrane potential;
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