부추의 에텔아세테이트추출액의 투여가 개의 혈액학적 변화에 미치는 영향

The effects of the Korea chinese chives ethyl acetate on hematological changes in dog

  • 박미정 (경상북도 가축위생시험소 동부지소) ;
  • 이성동 (경상북도 가축위생시험소 동부지소) ;
  • 김하동 (경상북도 가축위생시험소 동부지소) ;
  • 김주완 (경상북도 가축위생시험소 동부지소) ;
  • 송재찬 (경상북도 가축위생시험소 동부지소) ;
  • 김영홍 (경상북도 가축위생시험소 동부지소) ;
  • 오태호 (경상북도 가축위생시험소 동부지소) ;
  • 이근우 (경북대학교 수의과대학)
  • Park Mi-Jung (Eastern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Lee Sung-Dong (Eastern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Kim Ha-Dong (Eastern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Kim Joo-Wan (Eastern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Song Jae-Chan (Eastern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Kim Young-Hong (Eastern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Oh Tae-Ho (Eastern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Lee Keun-Woo (College of Veterinary Medicine, Kyungpook National University)
  • 발행 : 2004.12.01

초록

The Korea chinese chives are a major edible vegetable and they are traditionally been used as a folk medicine. However recently studies on the Allium tuberosum are mainly on the morphological property and the chemical constituent. There are no reports concerning hematological changes in dogs administrated ethyl acetate extracts of Korea chinese chive. This experiment was conducted to investigate the hematological changes in dogs administrated ethyl acetate extracts of the Korea chinese chives($5m{\ell}/mg$) for 6 days. During administration blood samples were collected at 3h, 6h, 12h and then on daily basis until day 6. Blood samples were analyzed red blood cell(RBC), packed cell volume(PCV), hemoglobin concentration(Hb), mean corpuscular volume(MCV), mean corpuscularl hemoglobin concentration(MCHC), reduced glutathion(GSH), and met-hemoglobin concentration. The RBC count was significantly(p<0.01, p<0.05) decreased during experimental periods. Packed cell volume, hemoglobin concentration were not showed significantly difference during experimental periods. The MCV was significantly(p<0.01, p<0.05) increased from 3 hours after administration to day 4. The MCHC was significantly(p<0.05)decreased on day 6. GSH concentration was not showed significant difference during experimental periods and the met-hemoglobin concentration was significantly(p<0.05) increased on day 3.

키워드

참고문헌

  1. 이우승. 1994. 한국의 채소. 경북대학교출판부. 대구: 371-380
  2. Park ER, Jo JO. 1998. Volatile flavor components of leek(Allium tuberosum Rottler). J Kor Soc Food Sci Nutr 27(4):563-567
  3. 농업정보신문. 1998. 월간 원예 168호 농업정보신문. 서울: 102-105
  4. Hong JH, Lee MH. 2000. Separation and identification of anti-microbial compounds from Korea leek (Allium tuberosum). J Food Hyg Safety 15(3): 235-240
  5. Kim SJ, Park KH. 1996. Antimicrobial substances in leek(Allium tuberosum). J Kor Food Sci Technol 28(3):604-608
  6. Choi JS, Kim JY. 1992. Isolation of adenosin and free amino acid composition from the leaves of Allium tuberosum. J Kor Soc Food Nutr 21(3):286-290
  7. 홍서아, 왕수경. 2000. 부추와 식이지방이 고지혈증 흰쥐의 혈액성상 및 혈소판 응집에 미치는 영향. 한국영양학회지 33(4):374-385
  8. Kwak YJ, Jun HJ. 1998. Modulation of anticarcinogenic enzyme and plasma teststeron level in male mouse fed leek-supplemented diet. Kor Soc Food Nutr 27:968-972
  9. Oh HW, Lee KW. 2002. Hematological effect of administration excessive amount of the Korea native Chinese chive extracts in small dogs. J Vet Clin 19(1) :61-65
  10. Kim JW, Lee KW. 2002. The hematological effect of excessive crude tuberosum in large dog. J Vet Clin 19(1) : 55-60
  11. Sebrell WH. 1930. An anemia in dogs produced by feeding onions. Public Health Rep 45:1175-1189
  12. Gruhzit OM. 1931. Anemia in dogs produced by feeding of the whole onions and onion products. Am J Med Sic 181 :812-815
  13. Farkas MC, Farkas JN. 1974. Hemolytic anemia due to ingestion of onions in a dog. J Am Anim Hosp Assoc 10:65-66
  14. Yoshimitsu M. 1977. High concentration of blood glutathion in dog with acute hemolytic anemia. Jap J Vet Sci 39:187-189
  15. Lees GE, Polzin DJ. 1979. Idiopathic Heinz body hemolytic anemia in the dog. J Am Anim Hosp Assoc 15:143-151
  16. Stallbaumer M. 1981. Onion poisoing in a dog. Vet Rec 108:523-524
  17. Harvey JW, Racker D. 1985. Experimental onion-induced hemolytic anemia in dog. Vet Pathol 22:387-392
  18. Ogawa E, Shinoki T, Akahori F, Masaoka T. 1986. Effect of onion ingestion on antioxidizing agents in dog erythrocytes. Jpn J Vet Sci 48(4):685-691
  19. Yamato O, Maede Y. 1992. Susceptibility to onion-induced hemolysis in dogs with hereditary high erythrocyte reduced glutathione and potassium concentration. Am J Vet Res 53:134-137
  20. Edwards CM, Belford CJ. 1996. Six cases of heinz body hemolytic anemia induced by onion and/or garlic ingestion. Aust Vet Practit 26(1):18-21
  21. Lincoln SD, Howell ME. 1992. Hematologic effects and feeding performance in cattle fed cull domestic onions(Allium cepa). JAVMA 200(8):1090-1094
  22. Hutchison TWS. 1977. Onions as a cause of heinz body anemia and death in cattle. J Can Vet 18:358-360
  23. Pierce KR, Joyce JR. 1972. Acute hemolytic anemia caused by wild onion poisoing in horses. JAVMA 160(3):323-327
  24. Edwards CB, Belford CJ. 1996. Six cases of Heinz body hemolytic anemia induced by onion and/or garlic ingestion. Aust Vet Practit 26:18
  25. Kobayahi K. 1981. Onion poisoning in the cat. Feline Fract 11:22-27
  26. Lee KW. 1999. Hematological changes in dogs administrated excessive galic extracts. Korean J Vet Clic Med 16(2):289-292
  27. Verhoeff J, Harjer R. 1985. Onion poisoning of young cattle. Vet Rec 117:497-498
  28. Osamu Y, Yoshimitsu M. 1994. Novel Heinz body hemolysis factors in onion(Allium cepa). Biosci Biotech Biochem 58:221-222
  29. 장우석, 김홍태, 진태원, 등. 1999. 소형견에서 마늘의 투여가 혈액상에 미치는 영향. 한국임상수의학회지 16(2):276-280
  30. Kim HT, Lee KW. 2001. Hematological changes in dogs admin-istrated excessive amount of water celery crude juice. J Vet Clic Med 18(2):105-110
  31. Kim HT, Lee KW. 2001. Hematological changes in dogs admin-istrated excessive amount of water celery concentrated extracts. J Vet Clic Med 18(2):111-115
  32. 안병만, 이근우. 2001. 과량의 미나리 생즙의 투여가 HK phenotype 진도견의 혈액상에 미치는 영향. 한국임상수의학회지 18(4) :334-340
  33. Van Kampen KR, James LF, Johnson AE. 1970. Hemolytic anemia in sheep fed wild onions. J Am Vet Med Assoc 156:328-332
  34. Beutler E. 1969. Drug induced hemolytic anemia. Pharmacological Reviews. 21:73-103
  35. Beutler E, Duron O, Kelly BM. 1963. Improved method for the determination of blood glutathion. J Lab Clic Med 61:882-888
  36. Spice RN. 1976. Hemolytic anemia associated with ingestion of onions in a dog. J Can Vet 17:181-183.36.Beutler E. 1969. Drug induced hemolytic anemia. Pharmacological Reviews. 21:73-103
  37. Lee KW, Osamu Y, Motoshi T, et al. 2000. Hematologic changes associated with the apprearance of encentrocytes after intragastric administration of galic extract to dogs. AJVR 61(11):1446-1450 https://doi.org/10.2460/ajvr.2000.61.1446
  38. Anderson AC, Gee W. 1985. Normal blood values in the beagle. Vet Med 135 :53
  39. Maede Y. 1977. High concentration of blood glutathion in dogs with acute hemolytic anemia. Jap J Vet Sci 39:187-189
  40. Maede Y, Kasai N, Taniguchi N. 1982. Hereditary high concentration of glutathione in canine erythrocytes associated with high accumulation of glutamate, glutamin and aspartate. Blood 59:883-889
  41. Shan XQ, AW TY, Jones DP. 1990. Glutathione-dependent protection against oxidative injury. Pharmacol Ther 47:61-71
  42. Wintebourn CC, Metodiewa D. 1994. The reaction of superoxide with reduced glutathione. Arch Biochem Biophys 314:284-290
  43. William HH, Michael JP, William BJ. 1974. Glutathione S-transferases. J Bio Chem 249(22):7130-7139
  44. Yamato O, Hayashi M, Kasai E. 1999. Reduced glutathione accelerates the oxidative damage produced by sodium n-propylthiosulfate is one of the causatative agents of onion-induced haemolytic anemia in dog. Biochim Biophy Acta 1427:175-182