Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Artemisiae Capillaris Herba on Anti-inflammatory Properties in RAW264.7 Cell Line

  • Kang Seong Yeob (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Kim Sung Hoon (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kim Su Myung (Department of Anatomy, College of Oriental Medicine, Daejeon University) ;
  • Namgoong Uk (Department of Neurophysiology, College of Oriental Medicine, Daejeon University) ;
  • Kim Dong Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
  • Published : 2004.12.01
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Abstract

The present study was performed to examine the possible anti-inflammatory effects of a herbal drug ASCH in RAW264.7 cell line. Inflammation was induced by LPS toxin treatment to RAW264.7 macrophage cell line. Increases in cytokine production such as IL-1β, IL-6. and IL-18, COX-2, NOS-Ⅱ (iNOS), and TNF-alpha were observed at mRNA level in the LPS-treated RAW264.7 cells. Measurement of IL-6, nitric oxide and the reactive oxygen species (ROS) showed increased production of these inflammation mediators. Treatment of ASCH effectively decreased IL-1β protein in a dose-dependent manner, and IL-6 and IL-18 were reduced at 100㎍/㎖ of ASCH concentration. NO production was also decreased by ASCH treatment. A slight inhibition for TNF-alpha in terms of protein, but not mRNA level was obtained by 100㎍/㎖ of ASCH treatment. ASCH treatment to normal RAW264.7 cells did not produce any cytotoxicity, indicting that the action of ASCH was selective to inflammatory cells. Thus, the present data suggest that ASCH may act as an important regulator to alleviate the inflammatory symptoms.

Keywords

References

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