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Effect of Artemisiae Capillaris Herba on Anti-inflammatory Properties in RAW264.7 Cell Line  

Kang Seong Yeob (Department of Pathology, College of Oriental Medicine, Daejeon University)
Kim Sung Hoon (Graduate School of East-West Medical Science, Kyunghee University)
Kim Su Myung (Department of Anatomy, College of Oriental Medicine, Daejeon University)
Namgoong Uk (Department of Neurophysiology, College of Oriental Medicine, Daejeon University)
Kim Dong Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.6, 2004 , pp. 1832-1842 More about this Journal
Abstract
The present study was performed to examine the possible anti-inflammatory effects of a herbal drug ASCH in RAW264.7 cell line. Inflammation was induced by LPS toxin treatment to RAW264.7 macrophage cell line. Increases in cytokine production such as IL-1β, IL-6. and IL-18, COX-2, NOS-Ⅱ (iNOS), and TNF-alpha were observed at mRNA level in the LPS-treated RAW264.7 cells. Measurement of IL-6, nitric oxide and the reactive oxygen species (ROS) showed increased production of these inflammation mediators. Treatment of ASCH effectively decreased IL-1β protein in a dose-dependent manner, and IL-6 and IL-18 were reduced at 100㎍/㎖ of ASCH concentration. NO production was also decreased by ASCH treatment. A slight inhibition for TNF-alpha in terms of protein, but not mRNA level was obtained by 100㎍/㎖ of ASCH treatment. ASCH treatment to normal RAW264.7 cells did not produce any cytotoxicity, indicting that the action of ASCH was selective to inflammatory cells. Thus, the present data suggest that ASCH may act as an important regulator to alleviate the inflammatory symptoms.
Keywords
Artemisiae Capillaris Herba; RT-PCR; ELISA reader; RAW264.7; inflammation; cytokine; nitric oxide;
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1 Paracetamol-inhibitable COX-2 /
[ Botting R. ] / J Physiol Pharmacol.
2 Modulation of macrophage activity by proteolytic enzymes. Differential regulation of IL-6 and reactive oxygen intermediates (ROIs) synthesis as a possible homeostatic mechanism in the control of inflammation /
[ Bryniarski K;Maresz K;Szczepanik M;Ptak M;Ptak W. ] / Inflammation.
3 Differential induction of IL-1<TEX>$\beta$</TEX> and TNF by CD40 ligand or cellular contact with stimulated T cells depends on the maturation stage of human monocytes /
[ Burger D;Molnarfi N;Gruaz L;Dayer JM. ] / J Immunol.
4 Inhibition of IFN-gamma-mediated inducible nitric oxide synthase induction by the peroxisome proliferator-activated receptor gamma agonist, 15-deoxy-delta 12,14- prostaglandin J2, involves inhibition of the upstream Janus kinase/STAT1 signaling pathway /
[ Chen CW;Chang YH;Tsi CJ;Lin WW. ] / J Immunol.
5 Signal transduction for inhibition of inducible nitric oxide synthase andcyclooxygenase-2 induction by capsaicin and related analogs in macrophages /
[ Chen CW;Lee ST;Wu WT;Fu WM;Ho FM;Lin WW. ] / Br J Pharmacol.
6 Interleukin-1, tumor necrosis factor and their specific inhibitors /
[ Dayer JM;Burger D. ] / Eur Cytokine Netw.
7 Nitric oxide-dependent activation of p53 suppresses bleomycin- induced apoptosis in the lung /
[ Davis DW;Weidner DA;Holian A;McConkey DJ. ] / J Exp Med.
8 Novel regulation of cyclooxygenase-2 expression and prostaglandin E2 productionby IFN-gamma in human macrophages /
[ Barrios-Rodiles M;Chadee K. ] / J Immunol.
9 Establishment of the role of IL-6 and TNF receptor 1 using gene knockout mice /
[ Bluethmann H;Rothe J;Schultze N;Tkachuk M;Koebel P. ] / J Leukoc Biol.
10 Biochemically based design of cyclooxygenase-2 (COX-2) inhibitors: facileconversion of nonsteroidal antiinflammatory drugs to potent and highly selectiveCOX-2 inhibitors /
[ Kalgutkar AS;Crews BC;Rowlinson SW;Marnett AB;Kozak KR;Remmel RP;Marnett LJ. ] / Proc Natl Acad Sci U S A.
11 Effect of retinoids on LPS- induced COX-2 expression and COX-2 associated PGE(2) release from mouse peritoneal macrophages and TNF- alpha release from ratperipheral blood mononuclear cells /
[ Kim BH;Kang KS;Lee YS. ] / Toxicol Lett.
12 Effects of Ginkgetin from Ginkgo biloba Leaveson cyclooxygenases and in vivoskin inflammation /
[ Kwak WJ;Han CK;Son KH;Chang HW;Kang SS;Park BK;Kim HP. ] / Planta Med.
13 Pharmacology of the oriental herbal drugs /
[ Kim, HK;Kim, HM;Song, BK;Le, UJ;Jung, HT. ] / Korea Medical Science
14 /
[ Kim, HC ] / Pharmacology in oriental medicine
15 Genetic ablation of inducible nitric oxide synthase decreases mouse lungtumorigenesis /
[ Kisley LR;Barrett BS;Bauer AK;Dwyer-Nield LD;Barthel B;Meyer AM;Thompson DC;Malkinson AM. ] / Cancer Res.
16 Distinct patterns of nitric oxide production in hepatic macrophages and endothelial cells following acute exposure of rats to endotoxin /
[ Laskin DL;Heck DE;Gardner CR;Feder LS;Laskin JD. ] / J Leukoc Biol.
17 The mode of action of aspirin-like drugs: effect on inducible nitric oxide synthase /
[ Amin AR;Vyas P;Attur M;Leszczynska-Piziak J;Patel IR;Weissmann G;Abramson SB. ] / Proc Natl Acad Sci U S A.
18 Airway inflammation is present during clinical remission of atopic asthma /
[ van den Toorn LM;Overbeek SE;de Jongste JC;Leman K;Hoogsteden HC;Prins JB. ] / Am J Respir Crit Care Med.
19 Inducible nitric oxide synthase inhibitors of Chinese herbs. Part 2: naturally occurring furanocoumarins /
[ Wang CC;Lai JE;Chen LG;Yen KY;Yang LL. ] / Bioorg Med Chem.
20 Inducible nitric oxide synthase inhibitor of the Chinese herb I. Saposhnikovia divaricata (Turcz.) Schischk /
[ Wang CC;Chen LG;Yang LL. ] / Cancer Lett.
21 Interleukin-1beta (IL-1beta) inhibition: a possible mechanism for the anti-inflammatory potency of liposomally conjugated methotrexate formulations in arthritis /
[ Williams AS;Jones SG;Goodfellow RM;Amos N;Williams BD. ] / Br J Pharmacol.
22 Resolution of inflammation /
[ Willoughby DA;Moore AR;Colville-Nash PR;Gilroy D. ] / Int J Immunopharmacol.
23 Superoxide and peroxynitrite generation from inducible nitric oxide synthase in macrophages /
[ Xia Y;Zweier JL. ] / Proc Natl Acad Sci U S A.
24 Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P /
[ Delgado AV;McManus AT;Chambers JP. ] / Neuropeptides.
25 Interleukin-18 /
[ Dinarello CA. ] / Methods.
26 Immunomodulatory effects of agents of plant origin /
[ Ganju L;Karan D;Chanda S;Srivastava KK;Sawhney RC;Selvamurthy W. ] / Biomed Pharmacother.
27 Cyclooxygenase-2 expression in macrophages: modulation by protein kinaseC-alpha /
[ Giroux M;Descoteaux A. ] / J Immunol.
28 Reactive oxygen intermediates as regulators of TNF-alpha production in rat lung inflammation induced by silica /
[ Gossart S;Cambon C;Orfila C;Seguelas MH;Lepert JC;Rami J;Carre P;Pipy B. ] / J Immunol.
29 Distinct contribution of IL-6, TNF-alpha, IL-1<TEX>$\beta$</TEX>, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice /
[ Hata H;Sakaguchi N;Yoshitomi H;Iwakura Y;Sekikawa K;Azuma Y;Kanai C;Moriizumi E;Nomura T;Nakamura T;Sakaguchi S. ] / J Clin Invest.
30 Antiinflammatory activity of the antirheumatic herbal drug, gravel root (Eupatorium purpureum): further biological activities and constituents /
[ Habtemariam S. ] / Phytother Res.
31 Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice /
[ Hata H;Sakaguchi N;Yoshitomi H;Iwakura Y;Sekikawa K;Azuma Y;Kanai C;Moriizumi E;Nomura T;Nakamura T;Sakaguchi S. ] / J Clin Invest.
32 Expression of inducible nitric oxide synthase and nitrotyrosine during the evolution of experimental pulmonary tuberculosis /
[ Hernandez-Pando R;Schon T;Orozco EH;Serafin J;Estrada-Garcia I. ] / Exp Toxicol Pathol.
33 Elevated interleukin 6 is induced by prostaglandin E2 in a murine model ofinflammation: possible role of cyclooxygenase-2 /
[ Hinson RM;Williams JA;Shacter E. ] / Proc Natl Acad Sci U S A.
34 Contribution of IL-1 beta and TNF-alpha to the initiation of the peripheral lung response to atmospheric particulates (PM10) /
[ Ishii H;Fujii T;Hogg JC;Hayashi S;Mukae H;Vincent R;van Eeden SF. ] / Am J Physiol Lung Cell Mol Physiol.
35 Monosodium urate crystals synergize with IFN-gamma to generate macrophage nitric oxide: involvement of extracellular signal-regulated kinase 1/2 and NF-kappa B /
[ Jaramillo M;Naccache PH;Olivier M. ] / J Immunol.
36 Pre-B cell colony-enhancing factor inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis /
[ Jia SH;Li Y;Parodo J;Kapus A;Fan L;Rotstein OD;Marshall JC. ] / J Clin Invest.
37 Role of IL-18 in acute lung inflammation /
[ Jordan JA;Guo RF;Yun EC;Sarma V;Warner RL;Crouch LD;Senaldi G;Ulich TR;Ward PA. ] / J Immunol.
38 Regulation of experimental autoimmune encephalomyelitis by interleukin-12 /
[ Leonard JP;Waldburger KE;Goldman SJ. ] / Ann N Y Acad Sci.
39 Anti-inflammatory effect of kamebakaurin in in vivo animal models /
[ Lee JH;Choi JK;Noh MS;Hwang BY;Hong YS;Lee JJ. ] / Planta Med.
40 Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 activity by 1,2,3,4,6-penta- O-galloyl-beta-D- glucose in murine macrophage cells /
[ Lee SJ;Lee IS;Mar W. ] / Arch Pharm Res.
41 A role for IL-18 in neutrophil activation /
[ Leung BP;Culshaw S;Gracie JA;Hunter D;Canetti CA;Campbell C;Cunha F;Liew FY;McInnes IB. ] / J Immunol.
42 Activation of macrophage CD8: pharmacological studies of TNF and IL-1 beta production /
[ Lin TJ;Hirji N;Stenton GR;Gilchrist M;Grill BJ;Schreiber AD;Befus AD. ] / J Immunol.
43 Cyclooxygenase-2 and inflammation in atherosclerosis /
[ Linton MF;Fazio S. ] / Curr Opin Pharmacol.
44 The key role of macrophages in the immunopathogenesis of inflammatory bowel disease /
[ Mahida YR. ] / Inflamm Bowel Dis.
45 Nitric oxide levels regulate macrophage commitment to apoptosis or necrosis during pneumococcal infection /
[ Marriott HM;Ali F;Read RC;Mitchell TJ;Whyte MK;Dockrell DH. ] / FASEB J.
46 Selectivity of nonsteroidal antiinflammatory drugs as inhibitors of constitutive and inducible cyclooxygenase /
[ Mitchell JA;Akarasereenont P;Thiemermann C;Flower RJ;Vane JR. ] / Proc Natl Acad Sci U S A.
47 Inhibition of TPA-induced cyclooxygenase-2 expression and skin inflammation in mice by wogonin, a plant flavone from Scutellaria radix /
[ Park BK;Heo MY;Park H;Kim HP. ] / Eur J Pharmacol.
48 The macrophage, TNF, and other cytokines /
[ Pass HI;Mew D;Pass HA;Temeck BK. ] / Chest Surg Clin N Am.
49 Inducible nitric oxide: an autoregulatory feedback inhibitor of vascular inflammation /
[ Peng HB;Spiecker M;Liao JK. ] / J Immunol.
50 The role of IFN regulatory factor-1 in synovitis and nitric oxide production /
[ Shiraishi A;Dudler J;Lotz M. ] / J Immunol.
51 Nitric oxide and host defense against Pneumocystis carinii infection in a mouse model /
[ Shellito JE;Kolls JK;Olariu R;Beck JM. ] / J Infect Dis.
52 Mechanistic analysis of the immunomodulatory effects of a catalytic antioxidant on antigen-presenting cells: implication for their use in targeting oxidation-reduction reactions in innate immunity /
[ Tse HM;Milton MJ;Piganelli JD. ] / Free Radic Biol Med.
53 Inducible nitric oxide synthase inhibitors suppress airway inflammation in mice through down-regulation of chemokine expression /
[ Trifilieff A;Fujitani Y;Mentz F;Dugas B;Fuentes M;Bertrand C. ] / J Immunol.
54 Reciprocal regulation of inflammation and lipid metabolism by liver Xreceptors /
[ Joseph SB;Castrillo A;Laffitte BA;Mangelsdorf DJ;Tontonoz P. ] / Nat Med.
55 Regulation of macrophage cytokine production by prostaglandin E2. Distinctroles of cyclo- oxygenase-1 and -2 /
[ Williams JA;Shacter E. ] / J Biol Chem.