Mass Production of Sand Dune Plant, Vitex rotundifolia via Micropropagation

  • 발행 : 2004.09.01

초록

The fruits of Vitex rotundifolia in Korea, known as 'Man Hyung Ja', occupy an important position as traditional oriental medicine in Asian countries. It is known that propagation of this plant by seed is difficult and time-consuming with little success. Attempts were made to develop a method by using nodal culture techniques. Explants of stem node without leaves cultured on Nitsch medium containing 1 ml/L BA, gave the best shoot induction ratio. Also, BA with IAA or TDZ treatment showed positive effect on shoot induction. Half-strength Nitsch medium was supplemented with 0.5 mg/L NAA produced better success than did the others on root formation. It showed that many of the regenerants grew successfully on growth chamber at $24^{\circ}c$.

키워드

참고문헌

  1. Al-Bahrany AM, Al-Khayri JM (2003) Micropropagation of grey mangrove Avicennia marina. Plant Cell Tiss Org Cult 72: 87-93
  2. Ashok Kumar HG, Murthy HN, Paek KY (2002) Somatic embryogenesis and plant regeneration in Gymnema sylvestre. Plant Cell Tiss Org Cult 71: 85-88
  3. Beena MR, Martin KP, Kirti PB, Molly H (2003) Rapid in vitro propagatlon of medicinally important Cempegia candelabrum. Plant Cell Tiss Org Cult 72: 285-289
  4. Cha HC, Park HJ, Min BM (2002) Plant regeneration and morphlogy during in vitro organogenesis from Heteniopsis oiientalis (Thunb.) C. Tanaka. J Plant Bio 45: 56-61
  5. Choi IM, Hyung NI, Kim SB (1992) Effects of medium compositions and on shoot multiplication and rooting in shoot tip cultures of 'Kyoho' and 'Campbell Early' grapes. Kor J Plant Tiss Cult 19: 59-66
  6. Clark G (1981) Plant anatomy and general botany, In Johansen DA, eds, Staining Procedures, Ed 4. Williams and Wilkins, London, pp 325-326
  7. Dronne S, Jullien F, Caissard JC, Faure 0 (1999) A simple and efficient method for in vitro shoot regeneration from leaves of lavandin (Lavandula $\times$ intermedia Emeric ex Loiseleur). Plant Cell Rep 18: 429-433
  8. Fernando SC, Gamage CKA (2000) Abscisic acid induced somatic embryogenesis in immature embryo explants of coconut (Cocos Nucifera L.) Plant Sci 151: 193-198
  9. Fellman CD, Read PE, Hosier MA (1987) Effects of thidiazuron and CPPU on meristem formation and shoot proliferation. Hortscience 22: 1197-1200
  10. Godo T, Kobayashi K, Takami T, Matsui K, Kida T (1998) In vitro propagation utilizing suspension cultures of meristematic nodular cell clumps and chromosome stability of Lilium $\times$ formolongi hort. Sci Hortic 72: 193-202
  11. Gray DJ, Benton CM (1991) In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundifolia). Plant Cell Tiss Org Cult 27: 7-14
  12. Han BH, Yae BW, Yu HJ, Sun JS (2001) In vitro propagation of Spathiphyllum floribundum cv. Cupid. Kor J Plant Tiss Cult 28: 209-213
  13. Jang SJ, Kim YH, Kim MK, Kim KW, Yun SE (2002) Essential oil composition from leaves, flowers, stems, and fruits of Vitex rotundifolia L. fil. J Korean Soc Agric Chem Biotechnol. 45: 101-107
  14. Jung YK (2000) The Viticetea rotundifoliae in South Korea and Japan. Korea J Ecology 23: 383-389
  15. Kawazoe K, Yutani A, Takaishi Y (1999) Aryl naphthalenes norlignans from Vitex totundifolia., Phytochem 52: 1657-1659
  16. Ko WG, Kang TH, Lee SJ, Kim NY, Kim YC, Sohn DH, Lee BH (2000) Polymethoxyflavonoids from Vitex rotundifolia inhibit proliferation by inducing apoptosis in human myeloid leukemia cells. Food and Chem Toxi 38: 861-865
  17. Konan NK, Schopke C, Carcamo R, Beachy RN, Fauquet C (1997) An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants and axillary bud-derived meristems. Plant Cell Rep 16: 444-449
  18. Lee TB (1982) Illustrated Flora of Korea. Hyangmunsa, Seoul
  19. Li W, Gao HH, Lu R, Guo GQ, Zheng GC (2002) Direct plantlet regeneration from the tuber of Stachys sieboldii. Plant Cell Tiss Org Cult 71: 259-262
  20. Nhut DT (1998) Micropropagation of lily (Lilium longiflorum). Plant Cell Rep 17: 913-916
  21. Nikam TD, Shitole MG (1997) In vitro plant regeneration from callus of niger (Guizotia abyssinica Cass.) cv. Sahyadri. Plant Cell Rep 17: 155-158
  22. Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phy tomorph 19: 389-404
  23. Ono M, Ito Y, Nohara T (1998) A labdane diterpene glycoside from fruit of Vitex rotundifolia. Phytochem., 48: 207-209
  24. Park HJ, Lee HL, Pyee JH, Cha HC (2001) Regeneration of grape (Vitis labruscana cv. Kyoho) by shoot-tip culture. J Plant Bio 44: 185-192
  25. Roberts AV, Smith EF, Mottley J (1990) The preparation of micropropagated plantlets for transfer to soil without acclimatization. In Pollard JW and Walker JM, eds, Plant Cell and Tissue Culture, Ed 6. Humana Press Inc, New Jersey, pp 228-229
  26. Romano A, Barros S, Martins-Loucao MA (2002) Micropropagation of the Mediterranean tree Ceratonia siliqua. Plant Cell Tiss Org Cult 68: 35-41
  27. Shin TY, Kim SH, Lim JP, Suh ES, Jeong HJ, Kim BD, Park EJ, Hwang wj, Rye DG, Baek SH, An NH, Kim HM (2000) Effect of Vitex rotundifolia on immediate-type allergic reaction. J Ethnopharm 72: 443-450
  28. Singh AK, Chand S, Pattnaik S, Chand PK (2002) Adventitious shoot organogenesis and plant regeneration from cotyledons of Dalbergia sissoo Roxb., a timber yielding tree legume. Plant Cell Tiss Org Cult 68: 203-209
  29. Torres KC (1989) Overview of facilities and Techniques, Torres K.C., eds, Tissue culture Techniques for Horticultural Crops. Van Nostrand Reinhold, New York, pp 52-53
  30. Ulrich MR, Davies FT, Koh YC, Duray SA, Egilla JN (1999) Micropropagation of Crinum 'Ellen Bosanquet' by tri-scales. Sci Horttic 82: 95-102
  31. Wawrosch C, Malla PR, Kopp B (2001) Clonal propagation of Lilium nepalense D.Don, a threatened medicinal plant of Nepal. Plant Cell Rep 20: 285-288