Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

In Vitro Regeneration of Lycium chinense Miller and Detection of Silent Somaclones with RAPD Polymorphisms

  • Ahn, In-Suk (Department of Forestry, North Carolina University) ;
  • Park, Young-Goo (Department of Forest, Kyungpook National University) ;
  • Shin, Dong-Ill (Department of plant genetic engineering, Daegu Catholic University) ;
  • Sul, Ill-Whan (Department of Biotechnology. Daegu University of Foreign Studies)
  • 발행 : 2004.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

An efficient system for the regeneration of adventitious shoots from in vitro cultured leaf sections of Lycium chinense Miller was developed and silent somaclones from the regenerants detected with RAPD method. Among the eight media tested (B5, SH, N&N, 1/2MS, MS, 3/2MS, GD and WPM), and four cytokinins (BA, kinetin, 2ip and zeatin) with different concentrations (1, 5, 10, 20, 30 and 40 $\mu{M}$), 1/2 MS medium supplemented with 20 and 30 $\mu{M}$ zeatin showed the best regeneration frequency (100% and 93.7%) and higher average number of shoots (9.0 and 9.4). All regenerants easily elongated after subculturing on 1/4MS without growth stimulants and produced spontaneous adventitious roots from their basal parts. With phenotypically normal 40 regenerants, RAPD analysis with 15 different random primers was performed to examine the cryptic somaclonal variants. No substantial differences in banding patterns were found in the amplified polymorphic DNAs implying no DNA changes during dedifferentiation into adventitious shoots. However, one (OPF-4) of the 15 primers detected silent somaclonal variation in one regenerant in which two different polymorphic bands did not appear when compared with the rest regenerants. The results indicate that regenerantion via intervening callus phase can be used to establish true-to-type planting stocks for homogeneous population.

키워드

참고문헌

  1. Bailey DC (1983) Isozyme variation and plant breeders' right. In: Tanksley SD, Orton TJ (eds) Isozymes in Plant Genetics and Breeding, Part A. pp 425-440. Elseview Science Publ. Amsterdam
  2. Chen WH, Chen TM, Fu YM, Hsieh RM, Chen WS (1998) Studies on somaclonal variation in Phalaenopsis. Plant Cell Rep 18: 7-13
  3. Chin YW, Lim, SW, Kim SH, Shin DY, Suh YG, Kim YB, Kim YC, Kim JW (2003) Hepatoprotective pyrrole derivatives of Lycium chinense fruits. Bioorg Med Chem Lett 13 1: 79-81
  4. Gamborg OL, Shyluk JP, Brar DS, Sonstabel F (1977) Morphogenesis and plant regeneration from callus of immature embryos of sorghum. Plant Sci Lett 10: 67-74
  5. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158
  6. Gesteira AS, Otoni WC, Barros EG, Moreira MA (2002) RAPDbased detection of genomic instability in soybean plants derived from somatic embryogenesis. Plant Breed 121: 269-271
  7. Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 17 161-170
  8. Hashmi G, Huettel R, Meyer R, Krusberg L, Hammerschlag F (1997) RAPD analysis of somaclonal variants derived from embryo callus cultures of peach. Plant Cell Rep 16: 624-627
  9. Hu Z, Yang J, Guo GQ, Zheng GC (2002) High-efficiency transformation of Lycium barbarum mediated by Agrobacterium tumefaciens and transgenic plant regeneration via somatic embryogenesis. Plant Cell Rep 21: 233-237
  10. Jang YP, Lee YJ, Kim YC, Hug H (1998) Production of a hepatoprotective cerebroside from suspension cultures of Lycium chinense. Plant Cell Rep 18: 252-254
  11. Kaneda Y, Tabei Y, Nishimura S, Harada K, Akihama T, Kitamura K (1997) Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogenesis in soybeans [Glycine max (L.) Merr.]. Plant Cell Rep 17: 8-12
  12. Konan NK, Schopke C, Carcamo R, Beachy RN, Fauquet C (1997) An efficient mass propagation system for cassava (Manihot esculents Crantz) based on nodal explants and axillary bud-derived meristems. Plant Cell Rep 16: 444-449
  13. Laparra H, Stoeva P, Ivanov P, Hahne G (1997) Plant regeneration from different explants in Helianthus smithii Heiser. Plant Cell Rep 16: 692-695
  14. Larkin PJ and Scowcroft WR (1981) Somaclonal variation - a novel source of variability from cell cultures for plant improvement. Theor App Genet 67: 433-455
  15. Lee T, Henag MEE, Pua E (1997) High frequency shoot regeneration from leaf disc explants of garland chrysanthemum (Chrysanthemum coronarium L.) in vitro. Plant Sci 126: 219-226
  16. Marie-Claude B, Bonal D, Goh DK and Monteuuis O (1998) Influcence of different macron utrient solutions and growth regulators on micropropagation of juvenile Acacia mangium and Paraserianthes falcataria explants. Plant Cell Tiss Org Cult 53: 171-177
  17. Murashige T and Skoog F (1962) Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497
  18. Ogura H (1990) chromosome variation in plant culture, In: YPS Bajaja (eds) Biotechnology in Agriculture and Forestry, Vol2: Somaclonal Variation in Crop Improvement. I. pp 49-84. Springer, Berlin, Heidelberg, New York
  19. Roy A, Frascaria N, Mackay J, Bousquet J (1992) Segregating random amplified polymorphic DNAs (RAPDs) in Betua alleghaniensis. Theor Appl Genet 85: 173-180
  20. Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199-204
  21. Shoyama Y, Zhu XX, Nakai R, Shiraishi S, Kohda H (1997) Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets. Plant Cell Rep 16: 450-453
  22. Sul IW, Korban SS (1998) Effect of media, carbon sources and cytokinins on shoot organogenesis in the christmas tree scots pine (Pinus sylvestris L.). J Hort Sci 73 6: 822-827
  23. Sul IW, Korban SS (1996) A highly efficient method for isolating genomic DNA from plant tissues. Plant Tiss Cult and Biotech 2: 113-116
  24. Tibok A, Blackhall NW, Power JB, Davey MR (1995) Optimized plant regeneration from callus derived from seedling hypocotyls of Eucalyptus urophylla. Plant Sci 110: 139-145