Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Prospective Evaluation of the G-protein $\beta$3 Subunit (GNB3) Gene 825T Polymorphism is Associated With Cerebral Infarction in Korean Population

한국인의 G Protein $\beta$3 Subunit유전자의 다형성과 뇌혈관 질환과의 상관성에 대한 연구

  • Choi Min Hee (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Lee Jin Woo (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Lee Kyung Jin (Department of Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Lee Hyo Jung (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Rho Sam Woong (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Choi Hyun (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Cho Ki Ho (Department of Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Hong Moo Chang (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Shin Min Kyu (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Kim Young Suk (Department of Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Bae Hyun Su (Department of Physiology, College of Oriental Medicine, Kyunghee University, Purimed R&D Institute)
  • 최민희 (경희대학교 한의과대학 생리학교실) ;
  • 이진우 (경희대학교 한의과대학 생리학교실) ;
  • 이경진 (경희대학교 한의과대학 심계내과학교실) ;
  • 이효정 (경희대학교 한의과대학 생리학교실) ;
  • 노삼웅 (경희대학교 한의과대학 생리학교실) ;
  • 최현 (경희대학교 한의과대학 생리학교실) ;
  • 조기호 (경희대학교 한의과대학 심계내과학교실) ;
  • 홍무창 (경희대학교 한의과대학 생리학교실) ;
  • 신민규 (경희대학교 한의과대학 생리학교실) ;
  • 김영석 (경희대학교 한의과대학 심계내과학교실) ;
  • 배현수 (경희대학교 한의과대학 생리학교실, 퓨리메드(주) 기업부설연구소)
  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Background and purpose: Hypertension and obesity has been implicated in the most important risk factors for stroke. The original finding that the G-protein beta3 subunit (GNB3) C825T allele associates with essential hypertension and obesity has been confirmed in several different populations. Hence, our objective was to determine whether the GNB3 C825T polymorphism predicts interindividual variation in stroke. Method: We recruited 361 stroke patients (cerebral infarction, n=278; intracerebral hemorrhage (ICH), n=83) and 199 healthy control subjects. Subjects were genotyped for GNB3 C825T mutation and findings were investigated for association with stroke. Result: The GNB3 T/T type was significantly associated with cerebral infarction prevalence (OR, 1.98; 95% Cl, 1.14-3.46; p=0.015). While, ICH was not found to be significantly associated with GNB3 T/T type (OR, 1.63; 95% ICH, 0.74-3.56; p=0.219). Similarly, no significant association was determined between GNB T/C type, and cerebral infarction (OR, 1.09; 95% Cl, 0.68-1.74; p=0.716), and ICH (OR, 1.14; 95% Cl, 0.59-2.21; p=0.697). Conclusion: In clinical characteristics, this study shows no differences among GNB3 genotypes, that are BMI, WH ratio. hypertension rate, and ischemic heart disease rate, total lipid level, triglycerides level, total cholesterol level, HDL cholesterol level, prothrombine time, with the exception of LDL cholesterol concentrations. However, our subjects showed an inverse relationship between LDL cholesterol level and the risk of cerebral infarction. We have shown that the GNB3 T/T genotype is strongly associated with cerebral infarction. (OR, 1.98; 95% Cl, 1.14-3.46; p=0.015).

Keywords

References

  1. 臨床中風學 김영석
  2. Stroke v.28 no.7 Stroke incidence and mortality correlated to stroke risk factors in the WHO MONICA Project. An ecological study of 18 populations. Stegmayr, B.;Asplund, K.;Kuulasmaa, K.;Rajakangas, A. M.;Thorvaldsen, P.;Tuomilehto, J.
  3. Korea National Statistical Office, Cases & deaths of communicable diseases
  4. 경희한의대논문집 v.2 no.1 中風의 病因論에 관한 文獻的 고찰. 장인규.
  5. 대한한방내과학회지 v.14 no.1 중풍증후의 동서의학적 비교고찰. 이화준;조기호;배형섭;김영석;이경섭
  6. Neuroepidermiology v.16 no.4 Stroke Prevention: windows of opportunity and failed expectations? A discussion of modifiable cardiovascular risk factors and a prevention proposal. Gorelick P. B.
  7. J Hypertens. v.14 no.3 Hypertention and Stroke. Straandgaard, S.
  8. Stroke v.29 no.2 Leisure-time physical activity and ischemic stroke risk: the Northern Manhattan Stroke Study. Sacco. R. L.;Gan, R.;Boden-Albala, B.;Lin, I. F.;Kargman, D. E.;Hauser, W. A.;Shea, S.;Paik, M. C.
  9. Stroke v.29 no.1 Risk factors for ischemic stroke in a Russian community: a poppulation-based case-control study. Feigin, V. L.;Wiebers, D. O.;Nikitin, Y. P.;O'Fallon, W. M.;Whisnant, J. P.
  10. N Engl J Med. v.341 no.15 Body-mass index and mortality in a prospective cohort of U.S. adults. Calle, E. E.;Thun, M. J.;Petrelli, J. M.;Rodriguez, C.;Heath, C. W. Jr.
  11. Lancet v.335 no.8693 Blood pressure, stroke, and coronary heart disease, part 2: effects of short term reductions in blood pressure: overview of randomised drug trials in their epidemiological context. Collins, R.;Peto, R.;MacMahon, S.;Hebert, P.;Fiebach, N. H.;Eberlein, K. A.;Godwin, J.;Qizilbash, N.;Taylor, J. O.;Hennekens, C. H.
  12. N Engl J Med. v.333 no.21 Primary prevention of stroke. Bronner, L. L.;Kanter, D. S.;Manson, J. E.
  13. Nat Genet. v.18 no.1 Association of a human G-protein β3 subunit variant with hypertension. Siffert, W.;Rosskopf, D.;Siffert, G.;Busch, S.;Moritz, A.;Erbel, R.;Sharma, A.;Ritz, E.;Wichmann, H.;Jakobs, K.;Horsthemke, B.
  14. Hypertension v.33 no.4 G-Protein β3 subunit C825T variant and ambulatory blood pressure in essential hypertension. Beige, J.;Hohenbleicher, H.;Distler, A.;Sharma, A. M.
  15. Hypertension v.32 no.3 Association between a polymorphism in the G-protein β3 subunit gene and lower renin and elevated diastolic blood pressure levels. Schunkert, H.;Hense, H. W.;Doling, A.;Riegger, G. A.;Siffert, W.
  16. J Am Soc Nephrol. v.10 no.9 Worldwide ethnic distribution of the G-protein β3 subunit 825T allele and its association with obesity in Caucasians, Chinese, and black African individuals. Siffert, W.;Forster, P.;Jockel, K. H.;Mvere, D. A.;Brinkmann, B.;Naber, C.;Crookes, R.;Du P Heyns, A.;Epplen, J. T.;Fridey, J.;Freedman, B. I.;Muller, N.;Stolke, D.;Sharma, A. M.;al Moutaery, K.;Grosse-Wilde, H.;Buerbaum, B.;Ehrlich, T.;Ahmad, H. R.;Horsthemke, B.;du Toit, E. D.;Tilikainen, A.;Ge, J.;Wang, Y.;Yang, D.;Husing, D.;Rosskopf, D.
  17. J Hypertens. v.17 no.8 G-protein β3 subunit 825T allele and its potential association with obesity in hypertensive individuals. Siffert, W.;Naber, C.;Walla, M.;Ritz, E.
  18. Genome Res. v.9 no.10 G-protein β3 subunit gene splice variant and body fat distribution in Nunavut Inuit. Hegele, R.A.;Anderson, C.;Young, T.K.;Connelly, P.W.
  19. Stroke v.26 no.8 Angiotensin-converting enzyme gene deletion polymorphism. A new risk factor for lacunar stroke but not carotid atheroma. Markus, H. S.;Barley, J.;Lunt, R.;Bland, J. M.;Jeffery, S.;Carter, N. D.;Brown, M. M.
  20. J Hypertens. v.13 no.12 Lack of association between angiotensin converting enzyme gene insertion/deletion polymorphism and stroke. Ueda, S.;Weir, C. J.;Inglis, G. C.;Murray, G. D.;Muir, K. W.;Lees, K. R.
  21. J Epidemiol. v.11 no.3 Apolipoprotein E genetic polymorphism and stroke subtypes in a Bangladeshi hospital-based study. Chowdhury, A. H.;Yokoyama, T.;Kokubo, Y.;Zaman, M. M.;Haque, A.;Tanaka, H.
  22. J Clin Invest. v.96 no.2 Enhanced G-protein activation in immortalized lymphoblasts from patients with essential hypertension. Siffert, W.;Rosskopf, D.;Merits, A.;Wieland, T.;Kaldenberg Stasch, S.;Kettler, N.;Nartung, K.;Beckmann, S.;Jakobs, K. H.
  23. Nephrol Dial Transplant v.15 no.9 G-protein β3 subunit 825T allele, hypertension, obesity, and diabetic nephropathy. Siffert, W.
  24. Clin Exp Hypertens v.21 no.5;6 World Health Organization - International Society of Hypertension Guidelines for the management of hypertension. Guidelines sub-committee of the World Health Organization. Chalmers, J.;MacMahon, S.;Mancia, G.;Whitworth, J.;Beilin, L.;Hansson, L.;Neal, B.;Rodgers, A.,;Ni Mhurchu, C.;Clark, T.
  25. World Health Organ Tech Rep Ser. v.727 Report of a WHO Study Group. Diabetes mellitus.
  26. Nucleic Acids Research v.19 no.19 A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies. Lahiri, D. K.;Nurnberger, J. I. Jr.
  27. 東醫心係內料學(下) 전국한의과대학 심계내과학교실
  28. 대한한의학회지 v.17 no.2 唐, 千金方에 기재된 中風에 관한 考察. 최은정;정승현;신길조;이원철
  29. Stroke v.13 no.1 Risk factors for cerebral hemorrhage and cerebral infarction in a Japanese rural community. Tanaka, H.;Ueda, Y.;Hayashi, M.;Date, C.;Baba, T.;Yamashita, H.;Shoji, H.;Tanaka, Y.;Owada, K.;Detels, R.
  30. Genome Res. v.6 no.4 A gene-rich cluster between CD4 and triosephosphate isomerase genes at human chromosome 12p13. Ansari-Lari, M. A.;Muzny, D. M.;Lu, J.;Lu, F.;Lilley, C. E.;Spanos, S.;Malley, T.;Gibbs, R. A.
  31. Hypertension v.37 no.2 C825T polymorphism of the G-protein 𝛽3 subunit and antihypertensive response to a thiazide diuretic. Turner, S. T.;Schwartz, G. L.;Chapman, A. B.;Boerwinkle, E.
  32. Hypertension v.36 no.1 G-protein β3 gene: structure, promoter, and additional polymorphisms. Rosskopf, D.;Busch, S.;Manthey, I.;Siffert, W.
  33. FEBS Lett. v.436 no.2 Enhanced fMLP-stimulated chemotaxis in human neutrophils from individuals carrying the G-protein β3 subunit 825 T-allele. Virchow, S.;Ansorge, N.;Rubben, H.;Siffert, G,;Siffert, W.
  34. 東醫病理學 安圭錫;崔昇勳;文濬典
  35. 東醫生理學 金完熙;申玟圭(外)
  36. 대한한방내과학회지 v.9 no.1 痰飮에 대한 文獻的 考察 洪天杓(외)
  37. 경희대학교 박사학위 논문 中風辨證診斷基準按에 대한 臨床的 연구 노진환
  38. 경희대학교 박사학위논문 뇌졸중 위험인자로서의 비만에 관한 임상적 연구 권도익
  39. J Epidemiol Community Health v.54 no.11 The relation between cholesterol and haemorrhagic or ischaemic stroke in the Renfrew/Paisley study. Hart, C. L.;Hole, D. J.;Smith, G. D.
  40. Stroke v.31 no.8 HDL-Cholesterol, total cholesterol, and the risk of stroke in middle-aged British men. Wannamethee, S. G.;Shaper, A. G.;Ebrahim, S.
  41. BMJ. v.309 no.6946 Influence of total cholesterol, high density lipoprotein cholesterol and triglycerides on risk of cerebrovascular disease: the Copenhagen City heart Study. Lindenstrom, E.;Boysen, G.;Nyboe, J.
  42. Neurochem Int. v.26 no.5 In vitro oxidation of vitamin E, vitamin C, thiols, and cholesterol in rat brain mitochondria incubated with free radicals. Vatassery, G. T.;Smith, W. E.;Quach, H. T;Lai, C. K.
  43. Free Radic Biol Med. v.22 no.3 Cholesterol: a two-edged sword in brain aging. Joseph, J. A.;Villalobos-Molinas, R.;Denisova, N. A.;Erat, S.;Strain, J.
  44. J Mol Cell Cardiol. v.26 no.5 The effect of sarcolemmal cholesterol content on the tolerance to anoxia in cardiomyocyte cultures. Lars Bastiaanse, E. M.;Van der Valk-Kokshoorn, L.J.M;Egas-Kenniphaas, J. M.;Atsama, D. E.;Van der Laarse, A.