Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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The Anticancer Effects and Immune Response on the Metastatic Lung Cancer by Wanpae-tang

완폐탕의 실험적 폐전이암에 대한 항암 및 면역효과에 관한 연구

  • Lee Seon Goo (Department of Pathology Oriental Medicine Sangji University) ;
  • Lee Dong Joo (Department of Pathology Oriental Medicine Sangji University) ;
  • Yoon Keun Chan (Department of Pathology Oriental Medicine Sangji University) ;
  • Ha Jee Yong (Department of Pathology Oriental Medicine Sangji University)
  • 이선구 (상지대학교 한의과대학 병리학교실) ;
  • 이동주 (상지대학교 한의과대학 병리학교실) ;
  • 윤근찬 (상지대학교 한의과대학 병리학교실) ;
  • 하지용 (상지대학교 한의과대학 병리학교실)
  • Published : 2003.06.01
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Abstract

Wanpae-tang is suggested to have antitumor activity on lung cancer. This study was performed to investigate antitumor, immune response, and apoptotic effects by Wanpae-tang in the cancer cell lines and C57BL/6 mice. Experimental studies were progressed through the anticancer activities such as, survival time, cell cytotoxicity, natural killer cell activity, productivity of interleukins and apoptotio effects. The results were summarized as follows: 1. Median survival time of Wanpae-tang treated group was prolonged to 4.1%, as compared with control group, but was not significant. 2. On the MTT assay, half-maximal inhibitory concentration(IC50) of Wanpae-tang was 15.00 ㎎/㎖ in HeLa cell, and 4.158 ㎎/㎖ in HRT-18 cell. 3. Natural killer cell activity in Wanpae-tang treated group was decreased in case of 100:1 and 10:1 effect cell/target cell ratio. 4. Production of interleukin-2, 4, 12 in Wanpae-tang treated group were significantly increased. 5. On the studies of Wanpae-tang induced apoptosis, a DNA fragmentation patterns were not appeared.

Keywords

References

  1. 立安散이 Angiogenesis 억제기전에 미치는 영향 이기룡
  2. Int. J. Radiat. Biol. v.743 no.4 The multi-step nature of carcinogenesis and the implication for risk analysis Cox R.
  3. 辨證施治槪要 白洪龍
  4. 歷代中醫腫瘤案論選粹 金桂淸
  5. 抗癌中藥一千方 郞偉君
  6. 東醫腫瘍學 崔昇勳
  7. 實用中醫內科學 上海中醫學院
  8. 實用腫瘤臨床 王拏玉
  9. 辨症錄 陳士鐸
  10. 辨證論治方藥合編 李尙仁;金東傑(校閱)
  11. Cancer chemotherapy Reports Protocol for screening chemical Agents and Natural products against animal tumors and other Biological system(Third Edition) R. I. Geran;N.H. Greenberg;M.M. Macdinald;A.M.Schumacher;B.J. Abbot.
  12. 大田大學校 韓醫學硏究所 論文集 v.5 no.2 肺癌의 辨證과 治方에 對한 文獻的 考察 裵基采;金聖勳
  13. M.S. Thesis Detection of mutations and human papillomavirus in human primary lung carcinoma by immunhistochemistry and ISH using biotinylated probes on paraffin embedded specimens. Park, I.W.
  14. 결핵 및 호흡기 질환 v.40 no.6 폐암에서 각종 진단수기에 따른 진단율에 관한 연구 강재송(외 7인)
  15. 종양학(개정판) 서울대학교 의과대학(편)
  16. Cancer v.3 Metastasis in carcinoma, analysis of 1000 autopsied cases Abrams H.L.;Spi개 R;Goldstein N.
  17. 대한외과학회지 v.56 no.1 위암의 폐전이 권성준;조석주
  18. 대한피부과학회지 v.25 no.2 내부 장기암의 피부전이 김유찬;조광현;이유신;함의근
  19. 處方構成을 위한 漢藥의 配合과 應用 梁基相
  20. 申氏本草學 申佶求
  21. 臨床本草學 辛民敎
  22. 臨床本草學 辛民敎
  23. 한국인삼사 한방에서 본 人蔘 李尙仁
  24. Int. J. Cancer v.16 Natural cytoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors Ⅲ, Characterization of effector cells Herberman R.B.;Nunn M.E.;Holden H.T.;Lavrin D.H.
  25. J. Exp. Med. v.149 Mechanism of rejection of virus persistently infected tumor cells by athymic nude mice Minato N.;Bloom B.R.;Jones C.;Holland J.;Reid L.M.
  26. Cell Immunol. v.160 Invitro reactivity of mouse natural killer(NK) cells against normal bone marrow cells Riccarch C.;Santoni A;Barlozzari T.;Herberman RB
  27. Adv. Cnacer Res. v.27 Natural cell-mediated immunity Herberman, R.B.;Holden, H.T.
  28. J. Immunol. v.138 Depletion of natural killer cells in mice by monoclonal antibody to NK-1. 1 : Reduction in defense against malignancy without loss of cellular or humoral immunity. Seaman W.E.;Sleienger M.;Eriksson E.;Koo G.C.
  29. Science v.193 Selective in vitro growth of T lymphocyte from normal human bone marrow Morgan D.A.;Ruscetti F.W.;Gallo R.C.
  30. Nature v.291 Interleukin-2 augments natural killer cell activity Henny C.S.;Kuribayashi K.;Mem D.E.;Gillis
  31. The cytokine Handbook Interleukin-4. Banchereau J.;Thomson A(eds.)
  32. J. Immunol. v.153 Recombinant interleukin 12 adminstration induced tumor regression in association with interferon-gamma production Hastala C.L.;Edington H.D.;Mckinncy T.G.;Tahara H.;Nalesnil M.A.;Brunda M.J.;Gately M.K.;Wolf S.F.;Schreiber R.D.;Storkus W.J.;Lotze M.T.
  33. Cancer Res. v.56 Interleukin 12 primes macrophages for nitric oxide production in vivo and restores depressed nitric oxide production by macrophage from tumor-bearing mice: implications for the antitumor activities of interleukin 12 and/or interleukin 2 Wigginton J.M.;Kuhns D.B.;Back T.C.;Brunda M.J.;Wiltrout R.H.;Cox G.W
  34. J. Exp. Med. v.170 Identification and purification of natural killer cell stimulatory factor(NKSF), a cytokine with multiple biologic effects on human lymphocytes. Kobayashi M.;Fitz L.;Hewick R.M.;Clark S.C.;Chan S.;Louden R.;Sherman F.;Perussia B.;Trinchieri G.
  35. Cell v.88 Programmed cell death in animal development Jacobson M.P.;Weil M.;Raff M.C.
  36. Biochem. v.32 Ca2+/Mg2+ Dependent endonuclease from human spleen, purification, properties, and role on apoptosis Ribeiro J.M.;Carson D.A.
  37. Int. J. Cancer v.75 Sequence-selective DNA cleavage by a topoisomerase I poison, NB-506 Fukasawa K.;Komatani H.;Hara Y.;Suda H;Okura A.;Nishimura S.;Yoshinari T.
  38. J. Biol. Chem. v.271 Poly(ADP-ribosyl)ation of Histone H1 correlates with jnternucleosomal DNA fragmentation during apoptosis. Yoon Y.S.;Kim J.W.;Kang K.W.;Kim Y.S.;Choi K.H.;Joe C.O.
  39. Cancer Res. v.51 Topoisomerase Ⅱ-reactive chemotherapeutic drugs induce apoptosis in thymocytes Walker P.R.;Smith C.;Youdale T.;Leblanc J.;Whitfield J. F.;Sikorska M.
  40. Nature v.380 no.6569 Requirement for ceramide-initiated SAPK/JNK signalling in stress-inducedapoptosis Verheij M.;Bose R.;Lin X. H.;Yao B.;Jarvis W. D.;Grant S.;Birrer M. J.;Szabo E.;Zon L. I.;Kyriakis J. M.;Haimovitz-Friedman A.;Fuks Z.;Kolesnick R. N.