Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Vasodilatory Effect of the Fractions from Crataegus pinnatifida and Isolation of the Active Component

산사 분획의 혈관이완 활성과 성분분석에 관한 연구

  • Choi Ho Jeong (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Shin Dong Hoon (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Kim Gil Whon (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Shin Heung Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
  • 최호정 (동국대학교 한의과대학 생리학교실) ;
  • 신동훈 (동국대학교 한의과대학 생리학교실) ;
  • 김길훤 (동국대학교 한의과대학 생리학교실) ;
  • 신흥묵 (동국대학교 한의과대학 생리학교실)
  • Published : 2003.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed for the investigation of vasodilatory effects of Crataegus pinnatifida Bunge and for isolation and structure determination of the constituent from the active fraction. The fruits of this herbal drug were extracted with 80% methanol, then fractioned successively with methylene chloride, ethylacetate and n-butanol. Among the fractions, ethyl acetate fraction exhibited the most effective vascular relaxation against phenylephrine-induced arterial contraction. In order to isolate the active constituent by activity-guided fractionation, this fraction was chromatographed on silica gel to yield seven subfractions. Among the subfractions, the active one showing the most potent vascular relaxation activity was further separated by prep. HPLC with reversed phase Microsorb C-18 column using 1 % acetic acid and methanol gradient solvent system to afford one pure compound, which revealed a potent vasodilatory effect. Instrumental analyses (NMR and mass spectrometry) of the isolated constituent indicated this compound to be (-)-epicatechin. The vasodilatory action mechanism of this compound should be further investigated.

Keywords

References

  1. 臨床配合本草 康秉秀;金永坂
  2. 本草綱目 李時珍
  3. Acta Ther. v.20 Hawthorn/Passionflower extract and impro- vement in physical capacity of patients with dyspnoea Class II of the NYHM functional classification M. von Eiff.
  4. Forschritte der medizin v.114 Therapeutic effectiveness of crataegus Weihmayr, T.;Emst, E.
  5. Arzneim-Forsch Drug Res. v.45 Myocadial effects of flavonoids from crataegus species Schussler, M.;Holzl, J.;Fricke, U.
  6. Therapiewoche v.33 The treatment f mild stble forms of angina pectoris using Ctataegutt novo Hanack, T.;Bruckel, M.H.
  7. 동의생리병리학회지 v.16 no.1 산사山楂의 혈관이완 효능과 항산화작용 손창우;채종구;김길훤;신흥묵
  8. 동의생리병리학회지 v.17 no.1 내피세포 Nitric Oxide 유리를 통한 山 의 혈관이완 작용 채종구;김길훤;신흥묵
  9. 동의생리병리학회지 v.17 no.2 산사 Butanol 분획이 PGF2a-유도 혈관평활근 수축의 억제에 미치는 신호전달 연구 유가량;최호정;김길훤;신흥묵
  10. 동국대학교 대학원 한의학과 석사학위논문 Effect of Butanol Fraction of Crataegi Fructus on the translocation of PKCa and myosin phosphotase subnits and LC20 phosphorylation in vascular smooth muscle 이헌제
  11. Toxicity, Plant medica v.43 Crataegus, toxicology and pharmacology. I. Ammon, H. P. T.;Hande,l M.
  12. Plant medica v.43 Crataegus, toxicology and pharmacology. II. Pharmacodynamics Ammon, H. P. T.;Handel, M.
  13. Plant medica v.4 Crataegus, toxicology and pharmacology. III. Pharmacodynamics and Pharmacokinetics Ammon, H. P. T.;Handel, M.
  14. Yingyang Xuebao v.23 no.1 Effect of hawthorn human vascular endothelial cell Chang, C.;Chen, J.
  15. PCt int. Appl. Cardoactive composition comprising L-carnitine and its derivatives and craataegus extracts Cavazza, C.
  16. Han'guk Nonghwa Hakhoechi v.36 no.3 Identification of phenolic antioxidative coponents in crataegus pinnatifida bunge Kim, J.S.;Lee, G.D.;Kwon, J.H.;Yoon, H.S.
  17. Journal of Nutritional Biochemistry v.12 Characterization of antioxidants present in hawthorn fruits Zhang, Z.;Chang, Q.;Zhu, M.;huang, Y.;Walter, K.K. Ho;Chen, Z.Y.
  18. Acta. Pharm. Sin. v.30 Analysis of the chemical constituents of hawthorn fruits and their quality evaluation Cao, G.Y.;Feng, Y.X.;Qin, X.Q.
  19. Lancet v.342 Dierary antioxidant flavonoids and risk of coronary heart disease: The Zutphen Elderly Study Hertog, M.G.L.;Feskens, E.J.M., Hollman, P.C.H., Katan, M.B., Kromhout, D.