Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Anticancer Effect of Combination with Paljinhangahm-dan and Adriamycin on HepG2 Human Malignant Hepatoma Cell Line

인간 간암세포주 HepG2에서 팔진항암단과 adriamycin의 병용처리에 의한 항종양 효과

  • Baek Eun Ki (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University) ;
  • Moon Goo (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University) ;
  • Won Jin Hee (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University) ;
  • Kim Dong Ung (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University) ;
  • Baek Dong Gi (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University) ;
  • Yoon Jun Chul (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University) ;
  • Song Bong Gil (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University) ;
  • Lee Byung Ho (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University) ;
  • Park Sang Gu (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University)
  • 백은기 (원광대학교 한의과대학 내과학교실) ;
  • 문구 (원광대학교 한의과대학 내과학교실) ;
  • 원진희 (원광대학교 한의과대학 내과학교실) ;
  • 김동웅 (원광대학교 한의과대학 내과학교실) ;
  • 백동기 (원광대학교 한의과대학 내과학교실) ;
  • 윤준철 (원광대학교 한의과대학 내과학교실) ;
  • 송봉길 (원광대학교 한의과대학 내과학교실) ;
  • 이병호 (원광대학교 한의과대학 내과학교실) ;
  • 박상구 (원광대학교 한의과대학 내과학교실)
  • Published : 2003.10.01
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Abstract

This study was designed to elucidate the synergistic cytotoxic mechanisms of the co-treatment of adriamycin and Paljinhangahm-dan in human hepatoma malignant cancer cell line, HepG2. The combination of adriamycin and the ethanol extract of Paljinhangahm-dan synergistically augmented the cytotoxicity of Adriamycin and Paljinhangahm-dan in HepG2 cells. The cytotoxicity of two drugs was revealed as apoptosis characterized by DNA fragmentaton in agarose gel electrophoresis. The apoptotic cytotoxicity of adriamycin and Paljinhangahm-dan was accompanied by the cleavage of procaspase -3 protease and PARP. Of note, anti apoptotic Bcl2 protein was obviously decreased, but Fas was dramatically increased in HepG2 cells co-treated with Adriamycin and Paljinhangahm -dan. In addition, through 2-D gel electorphoresis, we observed that the expression levels of a lot of proteins were obviously changed by the status of drug treatments. This results suggest that the synergistic cytotoxicity of the co-treatment of adriamycin and Paljinhangahm-dan might be caused by the changes of the expression levels of a lot of proteins which play pivotal roles in cell survival or death.

Keywords

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