생물환경조절학회지 (Journal of Bio-Environment Control)

  • 제12권4호
  • /
  • Pages.221-227
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  • 2003
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  • 1229-4675(pISSN)
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  • 2765-3641(eISSN)
한국생물환경조절학회 (The Korean Society for Bio-Environment Control)
권호 표지

Priming 처리에 의한 토마토 종자의 발아력과 Membrane Integrity에 미치는 영향

Effect of Seed Priming on the Germination Performance and Membrane Integrity of Tomato(Lycopersicon esculentum Mill.) Seeds

  • Kang, Jum-Soon (Deparment of Horticulture, Miryang National Unversity) ;
  • Son, Beung-Gu (Deparment of Horticulture, Miryang National Unversity) ;
  • Ahn, Chong-Kil (Deparment of Horticulture, Miryang National Unversity)
  • 발행 : 2003.12.01
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초록

본 연구에서는 토마토 종자의 priming 및 발아과정 중 세포막의 기능이 종자활력에 미치는 영향을 구명하고자 하였다. 토마토 종자의 적정 priming 처리제는 150 mL의 $KNO_3$였고, priming 처리된 종자는 발아촉진에 유효하였으며, 그 효과는 저온에서 현저하였다. $KNO_3$로 priming은 처리과정중 처리제에서 분리된 이온이 종자내로 이동하였다. Priming 처리과정중 전기전도도는 발아속도 단축에 가장 효과적이었던 $KNO_3$에서 처리개시 직후 약간 낮았다가 그 후 처리최종일까지 일정한 수준을 유지하였다. 발아기간중 용액의 전기전도도는 $KNO_3$ 용액으로 priming 처리된 종자에서는 낮았으나, $K_3PO_4$ 용액으로 priming 종자에서는 높게 나타났다. 발아촉진에 가장 효과적이었던 priming 처리제인 150 mL의 $KNO_3$ 용액으로 priming 처리하면 처리과정중 단백질, 아미노산, 가용성 당의 유출량은 $K_3PO_4$ 및 침지종자에 비해 낮았으며, 그 효과는 발아시에도 유지되었다.

The objective of this research was to determine the effect of seed priming on membrane integrity during poriming and germination. Among the five chemicals, $KNO_3$at 150 mL gave the shortest $T_{50}$ (days required to reach 50% of the final germination percentage). Compared to unprimed, the seeds primed with 150 mL $KNO_3$ at 20$^{\circ}C$ for 4 days had reduced $T_{50}$ values when germinated at 15$^{\circ}C$. These results indicated that seed priming is an effective way for rapid and synchronized germination, especially at low temperature. Changes in conductivity of priming solutions during the 4-days period of priming were highly dependent upon the priming agents. Conductivity of the $KNO_3$ and $K_3PO_4$ solution slowly declined during the first 3 hours ad then increased Amount of amino acids, sugars and proteins exuded from seeds into $KNO_3$ solution were less than those into distilled water and $K_3PO_4$. All the results suggested that the $KNO_3$ priming play a positive role in regulating the permeability of cell membranes.

키워드

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