Hydropriming Seed Treatment Improve Germinability of Cucumber

오이종자의 발아성 증진을 위한 Hydropriming 효과

  • Published : 2003.12.01

Abstract

Various hydration treatments have been devised to improve the rate and univormity of seed germination as well as seed viability. Hydropriming and priming, the imbibitaion of seeds in distilled water and osmotic solutions, respectively, are useful techniques for early establishment of uniform seedlings. In this study, the optimum conditions were determined for hysropriming of cucumber seeds. Effects of hydropriming were also compared to those of osmotic priming. Seeds, hydroprimed with distilled water and primed with 50 mM $NaNO_3$at 20 for 24 hour, showd shorter number of days required to reach 50% of the final germination percentage ($T_{50}$), and mean number of days to germinate (MDG) that those untreated seeds. Hydroprimed with 10 mL distilled water showed better results in improving the rate of germination thatn other treatments tested. Hydropriming of the aged seeds improved germination percentage at 15$^{\circ}C$ and 25$^{\circ}C$. The $T_{50}$ and MDG values were reduced significantly by hydropriming of the aged seeds regardless of germination temperature. In addition to being simple and inexpensive, the hydropriming resulted in early germination at low temperature. he hydropriming was also effective in increase of germination percentage and normal seedlings.

본 연구는 오이종자의 발아율 향상과 발아촉진을 위해 간편하고, 비용을 절감할 수 있는 hydropriming의 적정 환경조건을 구명하기 위해 수행되었다. 오이종자의 발아촉진에 최적 priming 처리제는 50 mL $NaNO_3$였다. 유근장 및 유근 건물중은 priming 처리제 종류에 따라 유의적인 차이는 없었으나, priming 및 hydropriming 종자는 무처리보다는 높았다. 그러나 50 mL $NaNO_3$와 hydropriming 처리간 발아력 증진 효과가 동일하여 오이종자에서는 물을 이용한 hydro-priming 처리가 처리비용을 절가할 수 있어 합리적인 종자처리였다. 오이종자의 hydropriming 적정 처리온도는 20$^{\circ}C$, 처리시간은 24시간이였다. Hydropriming시 종자 2g에 대한 용액량 10 mL 공급처리에서 발아율이 향상되었고 발아촉진에 좋아 적정 용액량이라고 판단되었다. 인위노화 종자는 발아율이 감소하였고 $T_{50}$ 및 MDG는 지연되었으며, 비정상적인 발아는 증가하였다. 활력이 저하된 조화종자를 ,hydropriming한 처리는 발아율이 향상되었고, 조기 발아하였다. 또한 비정상인 발아는 감소되었으며, 유근생장이 촉진되어 발아력이 부분적으로 회복되었다. 이러한 경향은 노화일수가 경과된 종자일수록, 부적당한 발아조건인 15$^{\circ}C$에서 현저하였다.

Keywords

References

  1. Association of Official Seed Analysis(AOSA), 1993, Rules for testing seeds. J. Seed Technol. 16:1-113
  2. Argerich, C.A., K.J. Bradford, and A.M. Tarquis. 1989. The effects of priming and ageing on resistance to deterioration of tomato seeds. J. Expl. Bot. 40:593-598 https://doi.org/10.1093/jxb/40.5.593
  3. Bradford, K.J., J.J. Steiner, and S.E. Trawatha. 1990. Seed priming influence on germination and emergence of pepper seed lots. Crop Sci. 30:718-721 https://doi.org/10.2135/cropsci1990.0011183X003000030049x
  4. Dearman, J., P.A. Brocklehurst, and R.L.K. Drew. 1986. Effects of osmotic priming and aging on onion seed germination. Ann. Appl. Biol. 108:639-648 https://doi.org/10.1111/j.1744-7348.1986.tb02003.x
  5. Elballa, M.M. 1987. Control of growth regulators and temperature on seedstalk development, seed yield and seed quality in carrots (Daucus carota L.). PhD. Diss. Univ. of Florida, Gainesville
  6. Guedes, A.C. and D.J. Cantliffe. 1980. Germination of lettuce(Lactuca sativa) at high temperature after seed priming. J. Amer. Soc. Hort. Sci. 105:777-781
  7. Haigh, A.M. and E.W. Barlow. 1987. Germination and priming of tomato, carrot, onion, and sorghum seeds in a range of osmotica. J. Amer. Soc. Hort. Sci. 111:660-665
  8. Heydecker, W. 1973/74. Germination of an idea: the priming of seeds. University of Nottingham School of Agriculture Peport. pp. 50-67
  9. Hujikura, Y., H.L. Kraak, A.S. Basra, and C.M. Karssen. 1993. Hydropriming, a simple and inexpensive priming merhod. Seed Sci Technol. 21:639-642
  10. Kang, J.S., J.L. Cho, and Y.O. Jeong. 1996. Morphological changes of primed and un-primed tomato (Lycopersicon esculentum Mill.) seeds during germination. J. Kor. Soc. Hort. Sci. 37:206-213
  11. Kang, J.S., Y.W. Choi, E.G. Son, C.K. Ahn, and J.L. Cho. 2000, Effect of hydropriming to enhance germination in gourd seeds. J. Kor. Soc. Hort. Sci. 41:559-564
  12. Khan, A.A. 1992. Preplant physiological seed conditioning, Hort. Rev. 13:131-181
  13. Kidd,F. and C. West. 1918. The influence of temperature on the soaking of seeds. New Phytologist 18:35-39 https://doi.org/10.1111/j.1469-8137.1919.tb07284.x
  14. Moon, B.S., Y.O. Jeong, and J.L. Cho. 1999. Seed treatment to improve germinability of gourd(Lagenaria siceraria Standl.) Kor. J. Hort. Sci. Technol. 17:747-749
  15. Suena, W. 1990. The role of vigor in the priming of tomato seeds. PhD thesis. Macquire Univ. Sydney, Australia
  16. Woodstock, L.W. and K.J. Tao. 1981. Prevention of imbibitional injury in low vigor soybean embryonic axes by osmotic control of water uptake. Physiol. Plant. 51-133-139 https://doi.org/10.1111/j.1399-3054.1981.tb00891.x