Radiation Oncology Journal

The Korean Society for Radiation Oncology (대한방사선종양학회)
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Radiation-induced Apoptosis in Developing Fetal Rat Cerebral Cortex

발육 중의 백서 태아 대뇌 피질에서 방사선에 의한 아포토시스

  • Chung Woong-Ki (Departments of Radiation Oncology Chonnam National University Medical School, University Hospital) ;
  • Nam Taek-Kehn (Departments of Radiation Oncology Chonnam National University Medical School, University Hospital) ;
  • Lee Min-Cheol (Departments of Fathology Chonnam National University Medical School, University Hospital) ;
  • Ahn Sung-Ja (Departments of Radiation Oncology Chonnam National University Medical School, University Hospital) ;
  • Song Ju-Young (Departments of Radiation Oncology Chonnam National University Medical School, University Hospital) ;
  • Park Seung-Jin (Departments of Medical Engineering Chonnam National University Medical School, University Hospital) ;
  • Nah Byung-Sik (Departments of Radiation Oncology Chonnam National University Medical School, University Hospital)
  • 정웅기 (전남대학교 의과대학 방사선종양학교실) ;
  • 남택근 (전남대학교 의과대학 방사선종양학교실) ;
  • 이민철 (전남대학교 의과대학 병리학교실) ;
  • 안성자 (전남대학교 의과대학 방사선종양학교실) ;
  • 송주영 (전남대학교 의과대학 방사선종양학교실) ;
  • 박승진 (전남대학교 의과대학 의공학과) ;
  • 나병식 (전남대학교 의과대학 방사선종양학교실)
  • Published : 2003.12.01
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Abstract

Purpose: This study was peformed to Investigate apoptosis by radiation In the developing fetal rat brain. Materials and Methods: Fetal blains were Irradiated In utero between the 17th and 19th days of fetal life (El7-19) by linear accelerator. A dose of Irradiation ranging from 1 Gy to 4 Gy was used to evaluate dose dependency. To test time dependency the ra)s were Irradiated with 2 Gy and then the fetal brain specimens were removed at variable 41me course; 1, 3, 5, 12 and 24 hours after the onset of irradiation. Immunohistochemlcal staining using in situ 707-mediated dUTP nick end labelling (TUNEL) technlfue was used for apoptotic cells. The cerebral cortex, including three zones on coriicai zone (Cf). Intermediate zone (if), and ventricular zone (VZ), was examined. Results : TUNEL positive cells revealed typical features of apoptotic cells under light microscope In the fetal rat cerebral cortex. Apoptotic cells were not found In the cerebral cortex of non-Irradiated fetal rats, but did appear In the entire cerebral cortex after 1 Gy Irradiation, and were more expensive at the ventricular and Intermediate zones than at the cortical zone. The extent of apoptosis was Increased with Increasing doses of radiation. Apoptosis reached the peak at S hours after the onset of 2 Gy Irradiation and persisted until 24 hours. Conclusion: Typical morphological features of apoplosis by irradiation were observed In the developing fetal rat cerebral cortex. It was more extensive at the ventricular and Intermediate zones than at the cortical zone, which suggested that stem cells or early differentiated cells are more radiosensitive than differentiated cells of the cortical zone.

목적: 발육 중인 백서 태아 대뇌 피질에서 방사선조사에 의한 아포토시스 반응에 대하여 알아보고자 하였다 대상 및 방법: 임신 백서의 태령 17 내지 19일(E17$\~$E19)에 선형가속기의 X-ray를 이용하여 어미 쥐의 복부에 방사선을 조사하였다. 방사선량에 따른 아포토시스를 보기 위하여 1, 2, 3, 4 Gy를 전후 조사면으로 방사선조사를 시행하고 5시간 후 백서 태아의 뇌를 획득하였다. 시간 경과에 따른 아포토시스를 보기 위하여 E7$\~$E19에 2 Gy의 방사선을 조사한 후 1, 3, 6, 12, 24시간에 각각 백서 태아의 뇌를 획득하였다. 대조군은 방사선을 조사하지 않은 임신 쥐를 같은 조건하에서 사육하여 각 군당 3마리씩 할당하였다. 아포토시스 세포는 면역조직화학적 방법(TUNEL, In situ 707-mediated dUTP nick end labeling)으로 염색하여 관찰하였다 결과: 백서 태아 대뇌 피질에서 TUNEL 양성인 세포는 광학현미경하에서 전형적인 아포토시스의 형태학적 특징을 나타내었다 대조군에서는 대뇌 피질의 전 층에서 TUNEL 양성세포를 거의 발견할 수 없었다. 1 Gy의 방사선 조사 후 5시간에 대뇌피질 전 층에서 미약하게 관찰되었으나 2 Gy에는 전 층에서 1 Gy 경우보다 더 증가하였고 그중 뇌실대와 중간대에서 피질대보다 더 많이 나타났다. 조사된 1$\~$4 Gy 범위에서 방사선량이 증가할수록 TUNEL양성세포가 더욱 증가하는 것을 볼 수 있었다. 2 Gy의 방사선 조사 후 3시간부터 TUNEL양성세포가 관찰 되기 시작하여 방사선 조사 후 6시간에 최고점을 이루었으며 이는 24시간까지 지속되었다. 결론: 발육 중인 백서 태아 대뇌피질에서 방사선에 의한 아포토시스의 전형적인 형태학적 특징을 관찰할 수 있었다. 아포토시스는 뇌실대와 중간대에서 피질대보다 더 많이 나타났으며 이는 줄기세포와 초기의 분화세포에서 방사선조사에 대한 감수성이 더욱 민감함을 시사하였다.

Keywords

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