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Radiation-induced Apoptosis in Developing Fetal Rat Cerebral Cortex  

Chung Woong-Ki (Departments of Radiation Oncology Chonnam National University Medical School, University Hospital)
Nam Taek-Kehn (Departments of Radiation Oncology Chonnam National University Medical School, University Hospital)
Lee Min-Cheol (Departments of Fathology Chonnam National University Medical School, University Hospital)
Ahn Sung-Ja (Departments of Radiation Oncology Chonnam National University Medical School, University Hospital)
Song Ju-Young (Departments of Radiation Oncology Chonnam National University Medical School, University Hospital)
Park Seung-Jin (Departments of Medical Engineering Chonnam National University Medical School, University Hospital)
Nah Byung-Sik (Departments of Radiation Oncology Chonnam National University Medical School, University Hospital)
Publication Information
Radiation Oncology Journal / v.21, no.4, 2003 , pp. 315-321 More about this Journal
Abstract
Purpose: This study was peformed to Investigate apoptosis by radiation In the developing fetal rat brain. Materials and Methods: Fetal blains were Irradiated In utero between the 17th and 19th days of fetal life (El7-19) by linear accelerator. A dose of Irradiation ranging from 1 Gy to 4 Gy was used to evaluate dose dependency. To test time dependency the ra)s were Irradiated with 2 Gy and then the fetal brain specimens were removed at variable 41me course; 1, 3, 5, 12 and 24 hours after the onset of irradiation. Immunohistochemlcal staining using in situ 707-mediated dUTP nick end labelling (TUNEL) technlfue was used for apoptotic cells. The cerebral cortex, including three zones on coriicai zone (Cf). Intermediate zone (if), and ventricular zone (VZ), was examined. Results : TUNEL positive cells revealed typical features of apoptotic cells under light microscope In the fetal rat cerebral cortex. Apoptotic cells were not found In the cerebral cortex of non-Irradiated fetal rats, but did appear In the entire cerebral cortex after 1 Gy Irradiation, and were more expensive at the ventricular and Intermediate zones than at the cortical zone. The extent of apoptosis was Increased with Increasing doses of radiation. Apoptosis reached the peak at S hours after the onset of 2 Gy Irradiation and persisted until 24 hours. Conclusion: Typical morphological features of apoplosis by irradiation were observed In the developing fetal rat cerebral cortex. It was more extensive at the ventricular and Intermediate zones than at the cortical zone, which suggested that stem cells or early differentiated cells are more radiosensitive than differentiated cells of the cortical zone.
Keywords
Radiation; Apoptosis; Rat; Brain;
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