KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Expression of $\beta$-amylase Gene and Degradation of Starch Granules of Germinating Rice Seed under Low Temperature and Submerged Soil Condition

저온.담수토양에서 벼종자 $\beta$-아밀라제 유전자 발현과 호분층 인접 배유의 전분분해 양상

  • 윤병성 (강원대학교 농업생명과학대학) ;
  • 강원희 (강원대학교 농업생명과학대학)
  • Published : 2002.12.01
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Abstract

This study was conducted to determine $\beta$-amylase gene expression and degradation of starch granules in the endosperm near scutellar epithelium of rice cultivars under the submerged soil at hypoxia 18$^{\circ}C$, which is practically important condition for farmers in temperate regions. In case of cv. Janghyangdo, accumulation of $\beta$-amylase mRNA was detected in the aleurone layer on the ninth day after seeding. However that of cv. Suwon 287 and Norm 6 were not detected in the aleurone layer in submerged soil(hypoxia) at 18$^{\circ}C$. $\beta$-amylase of cv. Janghyangdo was synthesized de novo in aleurone cells not in the scutellar epithelium. Degradation of starch granules in the endosperm near scutellar epithelium of c.v. Janghyangdo and Ginbozu, which have a strong $\beta$-amylase activity, was greater than that of cv. Suwon 287 and Norm 6 with no $\beta$-amylase activity in submerged soil(hypoxia) at 18$^{\circ}C$. This result may indicate that $\beta$-amylase gene expression and degradation of starch granules of germinating rice seed are related to the emergence of rice under the submerged soil condition at low temperature.

온대 수도작에 있어서 발아시의 조건에 가까운 저온.담수 토양조건에서의 출아에 관련된다고 생각되어지는 $\beta$-아밀라제 유전자 발현과 발현 양상이 실제로 호분층 인접 부분의 전분 분해에 영향을 미치는지 in situ hybridization과 현미화학적방법으로 검토하였다. 1. $18^{\circ}C$의 저온.담수토양조건하에서 출아했던 장향도 품종은 호분층에서 $\beta$-아밀라제 유전자의 발현이 보였다. 2. $18^{\circ}C$의 저온.담수토양조건하에서 출아하지 못했던 수원 287호는 $\beta$-아밀라제 유전자의 발현이 보이지 않았다. 3. $\beta$-아밀라제 활성의 유무에 의해 배반세포에 인접한 배유부분의 전분분해량에 변화를 보여 $\beta$-아밀라제 활성이 높은 장향도, 은방주(Ginbozu), Fortana I-133가 $18^{\circ}C$의 저온.담수토양조건하에서는 $\beta$-아밀라제 활성을 나타내지 않는 수원 287호와 시험한 모든 조건하에서 $\beta$-아밀라제의 활성이 보이지 않았던 농림(Norin) 6호, 고시히까리(Koshihikari) 보다 배반상피세포 및 배반상피세포에 인접한 호분층 근접 배유부분의 전분립 감소가 컸다. 이상의 결과 저온.담수토양조건하에서 벼 종자의 출아에 $\beta$-아밀라제 유전자의 발현과 전분 분해의 연관 가능성을 확인하였다.

Keywords

References

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