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Expression of $\beta$-amylase Gene and Degradation of Starch Granules of Germinating Rice Seed under Low Temperature and Submerged Soil Condition  

윤병성 (강원대학교 농업생명과학대학)
강원희 (강원대학교 농업생명과학대학)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.47, no.6, 2002 , pp. 413-417 More about this Journal
Abstract
This study was conducted to determine $\beta$-amylase gene expression and degradation of starch granules in the endosperm near scutellar epithelium of rice cultivars under the submerged soil at hypoxia 18$^{\circ}C$, which is practically important condition for farmers in temperate regions. In case of cv. Janghyangdo, accumulation of $\beta$-amylase mRNA was detected in the aleurone layer on the ninth day after seeding. However that of cv. Suwon 287 and Norm 6 were not detected in the aleurone layer in submerged soil(hypoxia) at 18$^{\circ}C$. $\beta$-amylase of cv. Janghyangdo was synthesized de novo in aleurone cells not in the scutellar epithelium. Degradation of starch granules in the endosperm near scutellar epithelium of c.v. Janghyangdo and Ginbozu, which have a strong $\beta$-amylase activity, was greater than that of cv. Suwon 287 and Norm 6 with no $\beta$-amylase activity in submerged soil(hypoxia) at 18$^{\circ}C$. This result may indicate that $\beta$-amylase gene expression and degradation of starch granules of germinating rice seed are related to the emergence of rice under the submerged soil condition at low temperature.
Keywords
$\beta$-amylase; gene expression; starch granules; submerged soil; rice germination.;
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