• Korean Journal of Food Science and Technology
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• v.42 no.6
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• pp.688-692
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• 2010

The aim of this study was to investigate the effects of molecular weight and concentrations of chitosan on the germination of brown rice. Brown rice was germinated at $30^{\circ}C$ for three days in various chitosan solutions. The germination rate of the brown rice increased with increasing concentrations of chitosan solution, and was higher in the chitosan solution than in water. GABA content increased with increasing germination time and chitosan solution concentration. As the molecular weight of the chitosan decreased, germination rate and GABA content increased in the brown rice. The GABA content of germinated brown rice using low molecular weight chitosan A in a 100 ppm solution was 5145.5 nmole/g. This is approximately a five times higher value than that of the water-germinated brown rice. Texture properties were enhanced in all the germinated brown rice samples in chitosan solution compared to the brown rice germinated in water. These results indicate that chitosan solution treatment can increase germination rate and GABA synthesis activity in brown rice during germination, and can also improve the texture properties of brown rice.

• Agricultural and Biosystems Engineering
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• v.7 no.1
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• pp.42-47
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• 2006

The objective of this study was to investigate the effects of air-phase germination with water-spraying and anion stimuli on germination ratio, sprout growth and ${\gamma}$-aminobutyric acid (GABA) of brown rice. Air-phase germination method with intermittent spraying water improved germination ratio and sprout growth by about 100% compared with the conventional water-soaking method. Anion radiation was applied during the germination process and improved the germination ratio, sprout growth and color quality of the germinated brown rice. Germination ratio and sprout growth were improved up to 9% with anion radiation, and its brightness was higher than brown rice germinated with no anion radiation. The air-phase germination with water-spraying improved the GABA content of germinated brown rice by about 8-9 times compared with that of brown rice.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.1
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• pp.11-14
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• 1998

This study was conducted to find out a desirable screening condition for the salinity tolerance in germination of rice. Seeds of 33 rice varieties were tested in NaCl solutions with various concentration levels. The germination percentage had a decreasing tendency with increasing NaCl concentration and inhibition concentration of 50% germination was 320mM. Standard deviation of germination percentage was highest (28.6) under 300mM NaCl. There was a highly significant correlation between the 50% germination concentration and the germination percentage at 20th day after seeding in 300mM NaCl. Also in 300mM NaCl, the germination percentage at 20th day after seeding was significantly correlated with the germination percentage at the 6th day after seeding. The salinity tolerance on the basis of germination percentage at 6th day after seeding in 300mM NaCl, was strong in 'Hyangnambyeo', 'Ilmibyeo', 'Kancheogbyeo', and 'Namwonbyeo', while weak in 'Ansanbyeo', 'Odaebyeo', 'Nonganbyeo', 'Dasanbyeo', and 'Namcheonbyeo'.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.287-294
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• 2006

Germination and early elongation of rice after germination were investigated in anoxic air treatment, nitric oxide gas treatment, and six concentrations of mercuric chloride solutions to determine the effects of limited oxygen environment, nitric oxide, and inhibited water flux through cell membrane in $17^{\circ}C$. Anoxic air treatment affected germination of tested six varieties very little. However root elongation rates were severely inhibited while shoot growth was affected less. Reductions in shoot and root elongations demonstrated genotypic variations. Nitric oxide delayed the germination of rice even though it didn't affect the final percent germination. Elongations of root and shoot were inhibited in nitric oxide treatment. The inhibitor effect of nitric oxide on the shoot elongation of rice was less severe, while nitric oxide completely inhibited the root emergence of rice. Concentrations of $HgCl_2$ greater than $300{\mu}M$ dramatically reduced the rate and percentage of germination when compared to distilled water treatment. The reduced percent germination showed the greatest variation among rice varieties in $500{\mu}M$ solution of mercuric chloride. Ansanbyeo, Jinheung, and Odaebyeo were affected less by $HgCl_2$, Nonganbyeo and Sangmibyeo were intermediate, and the germination of Andabyeo was greatly reduced by $HgCl_2$. Root elongation of germinated rice seedlings was more sensitive to oxygen deficits, nitric oxide, and $HgCl_2$ treatments than germination and shoot elongation. In conclusion, poor seedling establishment of rice sown in flooded paddy soils, in which the oxygen supply to the seeds is restricted, appears to the result of limited root elongation rate.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.3
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• pp.221-228
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• 2001

This study was carried out to investigate the effect of germination condition and drying temperature on growth and physicochemical properties of brown rice. Three brown rice seeds of Ilpumbyeo, Dasanbyeo and Heugjinjubyeo were stored at room temperature for six weeks to test the time-sequence germination viability. Relatively stable germination ratio was maintained until 2 weeks after storage. However, 3 weeks after storage, germination ratio of brown rice seeds started to decrease rapidly and their germination ratio was lower than 80%. For this reason, brown rice was recommended for seeding within 2 weeks after hulling. During the initial 5 days, germination ratio of 24 hours pre-soaking brown rice was higher about 2-3% than that of non-soaking brown rice. The $25^{\circ}C$ was considered as the most favorable temperature for brown rice germination, because of the high germination ratio and desirable coleoptile growth of the brown rice, and little seed rotting symptoms. The scanning electron micrographs showed the structural differences between hot-air dried and freeze dried germinated-brown rice kernel. In the freeze dried germinated-brown rice, seed coat (pericarp, tegmen and aleurone layer) was mechanically disrupted from the endosperm, and many cleavages were observed among starch storing cells and starch granules. The endosperm of freeze-dried brown rice kernels formed the sponge-like structures and showed the fragile traits. For this reason, hot-air drying is considered as more suitable method than freeze drying for germinated-brown rice. The crude protein and amylose contents were slightly changed, but there were no significant differences during the germination period. Crude fiber content was decreased, but crude Int and total amino acid contents were increased as seeding days increased. A rapid increase in $\alpha$-amylase activities of germinating brown rice was observed at S days after seeding, and $\alpha$-amylase activities were decreased from 8 days after seeding. Total free sugar contents were decreased during the germination period. There was continuous decline in the contents of sucrose and glucose until 8 days after seeding, but fructose and maltose content were gradually increased from the 5 days after seeding.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.1
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• pp.20-25
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• 2000

Viviparous germination causes yield loss and quality deterioration of rice. This study was conducted to investigate varietal differences of the viviparous germination with different days after heading (DAH) and different temperatures. In the laboratory examination, the averaged germination rate of all varieties at 45DAH and at 25DAH was 79.9%, and 27.5% under the incubation at the temperature of 3$0^{\circ}C$/2$0^{\circ}C$ (day/night) for 12 days. Andabyeo, Da- sanbyeo, and Nonganbyeo showed the lowest viviparous germination rates among the tested varieties. The shoot length of the viviparous germination measured 12days after incubation at 30/2$0^{\circ}C$ ranged from 21 to 53mm, indicating significant deterioration of rice quality. In the field test, the averaged viviparous germination rates of rice varieties at 25, 35, 45DAH with the underwater conditions for 4 days were 2.2, 6.2 and 9.2%, respectively, while their rates at 12 day after underwater conditions increased to 17.6, 44.2 and 43.8%, respectively. A variety that showed the highest viviparous germination rate at 25 and 35 and 45DAH was Heukjinjubyeo. When standing rice panicles without lodging were examined after consecutive raining for 7 days Juanbyeo showed the highest viviparous germination (45.5%), followed by Odaebyeo (16.0%), Jinbubyeo (14.5 %), Bongkwangbyeo (14.2 %) and Obongbyeo (12.6%). The viviparous germination of rice was greatly affected by variety, days after heading and temperature settings. Bongkwangbyeo, Juanbyeo, Dongjinbyeo, Hwasunchalbyeo, Naepoongbyeo and Heukjinjubyeo were classified as the most susceptible varieties to the viviparous germination in the field conditions.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.2
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• pp.187-192
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• 1994

This study was conducted to investigate varietal difference in viviparous germinability at different days after heading and temperature conditions. Three Japonica rice varieties, Sobaekbyeo, Odaebyeo and Hwaseongbyeo, and Sare type rice variety, Jakwangdo, showed higher viviparous germination rate from 20 days after heading, while a Tongil type rice, ]ungwonbyeo, and a Indica varieyt, IR-20, showed still low viviparous germination at 40 days after heading. Viviparous germination rate of rice varieties observed at 4 and 10 days after incubation was greately increased with increasing temperature from 15$^{\circ}C$ to 3$0^{\circ}C$ , and germination rate less than 5% was observed at 4 days after incubating at 15$^{\circ}C$ in all rice varieties teased. Viviparous germination pattern of rice varieties was divided into two types. Three Japonica and Sare type rice varieties showed higher and faster in viviparous germination than a Tongil type and Indica rice verieties. Viviparous germination was delayed under day /night fluctuation of incubation temperature compared with day /night fluctuation of incubation temperature compared with day /night constant condition at the same average temperature. The optimum incubation temperature and duration to evaluate viviparous germinability of rice varieties were 25/15$^{\circ}C$ and 6 days, respectively.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.269-269
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• 2017

Germination is one of the techniques used to enhance the texture properties and nutritional value of the brown rice (BR). Therefore, germinated BR (GBR) has received significant attention during the last decade. Physicochemical and cooking properties of brown rice were examined before and after germination. Germination raised the cooking properties, such as water absorption, expanded volume and soluble solid of cooked BR (brown rice). The texture, measured using tensipresser, was significantly improved by germination. The hardness of cooked BR was decreased by germination, but the GBR was sticker. In RVA, all viscosity value (peak viscosity, break down, set back, and final viscosity) of germinated rice flour was also reduced while gelatinization temperature did not change. Amylose content and amylopectin chain length distribution of BR starch were slightly changed by germination. Overall results revealed that germination was an effective tool to improve texture and cooking properties of BR.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.4
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• pp.316-320
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• 1999

Nine accessions of red rices collected from different regions were tested for germination characteristics in relation to seed maturity and several storage conditions after harvest. No red rice seeds germinated at five days after anthesis (DAA). Wanjuaengmi (long-grain red rice) seeds at 10DAA germinated up to 30% of total samples, producing only abnormal seedlings, whereas 10D-AA-seeds of normal cultivar showed only 3.3% germinability. Some red rice seeds absorbed more water than cultivated varieties, and showed less decrease in germination rate than cultivated varieties when seeds were exposed at -1, -5 or -1$0^{\circ}C$ for up to 60 hours after soaking. Red rice accessions maintained more than 95% germination when stored. indoor for 120 days. Two short-grain red rices showed about 50% germination when overwintered in the field, while other long-grain red rices and cultivated rices germinated less than 10%. Germination of seeds overwintered in clay loam soil was lower than that in loam soil, and seeds on surface germinated less than those in 1~7cm depth.

• Journal of Biosystems Engineering
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• v.36 no.3
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• pp.187-194
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• 2011

In this study an automatic germinating system for germinated brown rice (GBR) was developed for accelerating germination and sprouting of brown rice. With the developed germinating system, the effects of anion radiation treatment on germination ratio, sprout growth, color quality and ${\gamma}$-aminobutyric acid (GABA) content of brown rice were investigated. This system can fast produce GBR by controlling the environmental conditions such as ambient temperature, relative humidity, and proper anion radiation. Air-phase germination with intermittent water spraying improved both germination ratio and sprout growth by about 2.2 and 2.3 times, respectively, compared to the conventional water soaking method. Our results strongly suggest that this germinating system that provides air-phase germination with intermittent water spraying and anion radiation can produce germinated brown rice with good color quality, high GABA content, and fast germination ratio as well as sprout growth.