식이에 따른 장내세균의 효소활성 및 장내세균층의 비교

Fecal Microflora of Mice in Relation to Diet

  • 발행 : 1999.06.01

초록

식이에 따른 장내세균의 균총의 변화와 장내미생물의 효소활성을 비교하였다. 동물성 식이를 실시한 군에서는 식물성 식이를 실시한 군과 비교시 유해한 혐기성 균의 수가 약 10배 정도 많게 관찰되었으며, 식물성 식이를 실시한 군에서는 비피더스균이나 유산간균의 수가 동물성 식이를 실시함 군보다 10배 정도 많게 관찰되었다. 또한 대장암 발병과 상관관계가 깊은 것으로 알려진 장내미생물 유래효소활성을 비교한 결과 $\beta$-glucosidase, $\beta$-glucuronidae, tryptophanase, urease 등의 효소활성이 동물성 식이를 실시한 군에서 1.8배-2배 가량 높게 나타났다. 즉 식이에 따라 장내 세균총의 차이 및 장내세균의효소활성의 현저한 차이가 있음을 알 수 있었다.

The effects of diet on the composition of fecal microflora in mouse and the aclivilies of several enzymes in the leces were investigated. Vegetarian dietary groups were found to contain about ten lines higher numbers of Locmbocillus and B$&bacterizml than animal dietary groups. An~rnal dietmy groups were found to contain about 5 tolo times higher numbers of anaerobic Closhidia and Bocieriocles than the vegeterian detary groups. Fccal microbial $\beta$-glucosidase, $\beta$-glucm'onidase, ii-yptophanase and orease activilies in ihe animal dietary groups were shown lo be 30 to 50% hgher than those in h e vegetarian detary groups.

키워드

참고문헌

  1. Cancer v.34 The geographic pathology of bowel cancer Berg, J.W.;M.A. Hawell
  2. J. Bacteriol. v.109 Escherichia coli tryptophanase in the enteric environment Bostford, J.L.;R.D. Deomoss
  3. J. Nat'l Cancer Inst. v.54 Large bowel cancer: an epidemiologic jigsaw puzzle Burkitt, D.P.
  4. J. Surgical Oncology v.24 Induction of colon mucosal β-glucuronidase production as a mechanism for 1,2-Diemthylhydrazine colon carcinogenesis Celika,C.D.;A. Lewis;A. Middleman
  5. J. Natl. Cancer Inst. v.54 Tryptophanase of fecal flora as a possible factor in the etiology of colon cancer Chung, K.T.;G.E. Funk;M.W. Slain
  6. J. Natl. Cancer Inst. v.66 The cause of cancer;quantitative estimates of avoidable risk of cancer in the U.S. today Doll, R.;R. Peto
  7. Br. J. Cancer v.27 Environmental factor and cancer of the colon an breast Drassar, B.S.;D.Irving
  8. Cancer Res. v.35 Fecal bacteriology of colonic polyp patients and control patients Finegold, S.M.;D.J.A. Flora
  9. β-Glucosidase, methods of enzymatic analysis (2nd ed.) Fishman, W.H.
  10. β-Glucosidase, methods of enzymatic analysis (2nd ed.) Fishman, W.H.
  11. Urea, In Methods of enzymatic analysis v.4 Gutmann, I;H.U. Bergmeyer;Bergmeyer, H.U.(eds.)
  12. Biol. Pharm Bull. v.40 pH inducide β-glucosidase and β-glucuronidase of alkalotolerant intestinal bacteria Kim, D.H.;H.J.Sang;S.W.Kim;K. Kobayashi
  13. Adult disease and intestinal bacteria Kim, D.H.;M.J. Han
  14. Science v.199 β-Glucuronidase catalized hydrolysis of benzo-[α]-pyrene-glucuronide and binding to DNA Kinoshita, N.;H.V. Gelvoin
  15. Lancet v.30 Dietary fiber, transtime, fecal bacteria, steroids and colon cancer in two Scandinavian populations LARC
  16. J. Microbiol. v.132 Protein degradation by human intestinal bacteria Macfalane, G.F.;J.H. Cummings;C. Allison
  17. J. Japan. Infect. Dis. v.45 The method of screening for intestinal flora Mitsuoka, T.
  18. Cancer Res. v.35 Discussion of current bacteriological investigations of the relationships between intestinal flora, diet and colon cancer Moore, W.E.C.;L.V.Holdeman
  19. J. Natl. Cancer Inst. Large bowel carcinogenesis-fecal constituents of populations with diverse incidences rates of colon cancer Reddy,B.S.;E.L. Wynder
  20. Cancer v.39 Metabolic epidemiology of colon cancer Reddy, B.S.;E.L. Wynder
  21. Lancet v.16 High colonic pH promotes colorectal cancer Thorton, J.R.
  22. Food Technology v.48 Health effect of oligosaccharide Tomonatsu, H.
  23. New Eng. J. Med. v.323 Relation of meat, fat, fiber intake to the risk of colon cancer in a prospective study among women Willet, W.C.;M.J. Stamofer;G.A.Colditz;B.A.Rosner;F.E. Spizer