Journal of Korean Academy of Oral and Maxillofacial Radiology (치과방사선)

Korean Academy of Oral and Maxillofacial Radiology (대한영상치의학회)
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TUMOR-ASSOCIATED PROTEINS IN RAT SUBMANDIBULAR GLAND INDUCED BY DMBA AND IRRADIATION

DMBA 매식과 방사선 조사로 유발된 백서 악하선 암에 존재하는 단백질에 관한 연구

  • Oh Sung-Oak (Department of Oral and Maxillofacial Radiology, College of Dentistry, Seoul National University) ;
  • Choi Soon-Chul (Department of Oral and Maxillofacial Radiology, College of Dentistry, Seoul National University) ;
  • Park Tae-Won (Department of Oral and Maxillofacial Radiology, College of Dentistry, Seoul National University) ;
  • You Dong-Soo (Department of Oral and Maxillofacial Radiology, College of Dentistry, Seoul National University)
  • 오성욱 (서울대학교 치과대학 구강악안면방사선학 교실) ;
  • 최순철 (서울대학교 치과대학 구강악안면방사선학 교실) ;
  • 박태원 (서울대학교 치과대학 구강악안면방사선학 교실) ;
  • 유동수 (서울대학교 치과대학 구강악안면방사선학 교실)
  • Published : 1997.08.01
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Abstract

This study was performed in order to identify changes of the plasma membrane proteins in rat submandibular gland tumors induced by 7,12-dimethylbenz[a]anthracene [DMBA] and X-irradiation. Two kinds of tumor associated membrane proteins (protein A and B) were isolated with 3 M KCl extraction from rat submandibular gland tumors induced by DMBA and X-irradiation. To identify their antigenicities, immunoelectrophoresis and double immunodiffusion was carried out with various proteins extracted from liver, heart, skin and pancreas of adult rats and from embryonic liver, heart and skin. The rabbit antisera against the protein A did not cross-react with any of the proteins extracted from the above mentioned tissues, suggesting that protein A might be tumor specific antigen. However, the rabbit antisera against protein B was precipitated with proteins extracted from the liver of adult and embryonic rats. Polyacrylamide gel electrophoresis of these two proteins (A and B) showed that protein A was a dimer with molecular weights of 69,000 and 35,000 dalton, whereas protein B was a monomer with molecular weight of 50,000 dalton.

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