Regeneration of Immune System after Syngeneic Bone Marrow Transplantation in Irradiated Mice: II. B Lymphocyte Regeneration and Antibody Production

마우스 동계골수이식 후 면역체계의 재생: II. B림프구의 재생 및 항체산생

  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Oh, Heon (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Song-Eun (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Soon-Tae (Kyungpook Veterinary Service Laboratory) ;
  • Jo, Sung-Kee (Korea Atomic Energy Research Institute) ;
  • Hyun, Byung-Hwa (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ryu, Si-Yun (College of Veterinary Medicine, Chungnam National University) ;
  • Daynes, Raymond A. (School of Medicine, University of Utah)
  • Published : 1997.04.01

Abstract

Lethally irradited C3H/HeN mice were transplanted with syngeneic bone marrow. The B cell regeneration levels of spontaneous serum Ig, fecal igA and specific ig to diphtheria toxoid were determined at various time points. The number of B220+ cells reached normal range at 4 weeks after bone marrow transplantation(BMT) in spleen and lymph node. The B cell number of spleen returned to normal relatively soon than in the lymph node. Within 5 to 7 weeks after BMT, the transplanted mice contained nearly normal levels of spontaneous serum IgA, IgG2b and fecal IgA, but 2 fold lower levels of serum IgG2a, IgM and IgG3. Especially IgG3 levels were within low-normal range throughout the study. One to two weeks after immunization the predominant anti-diphtheria toxoid subtype was IgM. The levels of specific serum Ig were very low and after booster immunization at week 6, the short-lasting increase of Ig production was notd.

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