The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Ultrastructural Aspects of Nuclear Behaviors of Pleurotus ostreatus - Behaviors of Astral Microtubules During Hyphal Development -

느타리버섯균의 핵의 동태에 관한 미세구조적 연구 -균사분화중의 성상체 미세소관에 관한 연구-

  • 윤권상 (강원대학교 미생물학과)
  • Published : 1996.03.30
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Abstract

Premitotic, mitotic and postmitotic nuclei in the dikaryotic somatic hyphae of Pleurotus ostreatus, the oyster mushroom fungus were ultrastucturally examined using chemical fixation and freeze-substitution process, and the behaviors of astral microtubules associated with these nuclei were closely analyzed. Electron microscopic examinations revealed that astral microtubules are significantly abundant when the nuclei are in the stage of migration and at the stage of migration, the separation of spindle pole body occurs. Such an abundancy of astral microtubules in premitotic migrating nuclei is well contrasted with mitotic and postmitotic nuclei with much fewer astral microtubules and it should be noted that neither of these latter classes of nuclei exhibits the separation of the spindle pole body. It is remarkable that the postmitotic nuclei that are believed to migrate actively are associated with the astral microtubules that are less in numbers and length. During all the stages of nuclear division, astral microtubules are invariably radiating from the spindle pole bodies and nucleolus remains within the nuclear envelope of dividing nuclei throughout the division. The functions of astral microtubules developed during the nuclear division as well as the nuclear migration and separation of the spindle pole body were closely examined.

느타리버섯균의 이핵체 체세포 균사에서 분열전 및 분열후의 핵과 분열중의 핵을 화학고정방법과 동결교체방법을 이용하여 균사분화과정중의 성상체 미세소관의 미세구조적 동태를 조사하였다. 많은 수의 성상체 미세소관들이 이동중인 분열전의 핵들과 연관되어 있었으며, 분열전의 핵이 이동하는 시기에 SPB의 분리가 진행됨이 발전되었다. 분열전 이동중의 핵과는 대조적으로 SPB의 분리가 더 이상 일어나지 않는 분열중의 이거나 분열이 끝난 핵에서는 성상체미세소관이 덜 발달되어 있었다. 활발히 이동할 것으로 추측되는 분열후의 핵에서 핵과 연관된 성상체미세소관이 잘 발달되어 있지 않음은 특이한 점이다. 분열중의 모든 핵에서 성상체미세소관이 SPB에서 뻗어나오며 인은 핵막에 남아있다. 핵의 분열과 핵의 이동 및 SPB의 분리와 관련된 성상체미세소관의 기능에 대하여 논의하였다.

Keywords

References

  1. Cell Motility and the Cytoskel v.19 Detection of spindle pushing forces in vivo during anaphase B in the fungus Nectria haematococca Aist, J.R.;Bayles, C.J.
  2. Protoplasma v.161 Ultrastructural basis of mitosis in the fungus Nectria haematococca. I. Asters Aist, J.R.;Bayles, C.J.
  3. J. Cell Sci v.100 Direct experimental evidence for the existence, structural basis and function of astral forces during anaphase B in vivo Aist, J.R.;Bayles, C.J.;Tao, W.;Berns, M.W.
  4. J. Cell Sci v.95 What makes microtubules dynamic? Bayley, P.M.
  5. J. Cell Biol v.107 Astral forces in fungal mitosis Bayles, C.J.;Aist, J.R.;Tao, W.;Berns, M.W.
  6. Botanica Marina v.34 Relationships between the movements of chloroplasts and cytoskeletons in diatoms Chen, S.T.;Li, C.W.
  7. J. Biol. Chem. v.264 Quantitative analysis of sea urchin egg kinesin-driven microtubule motility Cohn, S.A.;Ingold, A.L.;Sholey, J.M.
  8. Chromosoma v.93 In vivo effects of orthovanadate on spindle structure and dynamics of locust spermatocytes. I. Daub, A.M.;Hauser, M.
  9. Biochemistry of Plants v.15 Cytoskeletal proteins and their genes in higher plants Fosket, D.E.
  10. vignae. J. Cell Biol. v.70 Ultrastructure of mitosis in the cowpea rust fungus Uromyces phaseoli var Heath, I.B.;Heath, M.C.
  11. J. Cell Sci. v.93 Conformation and movement of the vegetative nucleus of the angiosperm pollen tube: association with the actin filament Heslop-Harrison, J.;Heslop-Harrison, Y.
  12. Sex. Plant Repro v.2 Actomyosin and movement in the angiosperm pollen tube: an interpretation of some results Heslop-Harrison, J.;Heslop-Harrison, Y.
  13. J. Cell Sci. v.94 Myosin associated with the surfaces of organelles, vegetative nuclei and generative cells in angiosperm pollen grains and tubes Heslop-Harrison, J.;Heslop-Harrison, Y.
  14. Sex. Plant Repro v.5 Intracellular motility, the actin cytoskeleton and germinability in the pollen of wheat Heslop-Harrison, J.;Heslop-Harrison, Y.
  15. J. Cell Sci. v.94 Cytoskeletal interactions with postmitotic migrating nuclei in the oyster mushroom fungus, Pleurotus ostreatus: evidence against a force generating role for astral microtubules Kaminskyj, S.;Yoon, K.S.;Heath, I.B.
  16. Mechanismm of anaphase B movement;Biological functions of microtubules, and related structures Kronebush, P.J.;Borisy, G.G.;Sakai, H.(ed.);Mohri, H.(ed.);Borisy, G.G.(ed.)
  17. Intern. J. Parasitology v.18 The role of the cytoskeletal protein, tubulin, in the mode of action and mechanism of drug resistance to benzimidazole Lacey, E.
  18. Europ. J. Phycol. v.28 The arrangement of actin bundles and chloroplasts in the nodal regions of Characean internodal cells McLean, B.;Juniper, B.E.
  19. Protoplasma v.139 The cytoskeleton of the giant coenocytic green alga Caulerpa visualized by immunocytochemistry Menzel, D.
  20. Can. J. Bot. v.56 Fine structure of shitake, Lentinus edodes. VI. Cytoplasmic microtubules in relation to nuclear movement Nakai, Y.;Ushiyama, R.
  21. J. Cell Biol. v.109 The motor for poleward chromosome movement in anaphase is in or near the kinetochore Nicklas, R.B.
  22. J. Cell Sci. v.94 Mechanically cut mitotic spindles: clean cuts and stable microtubules Nicklas, R.B.;Lee, G.M.;Rieder, C.L.;Rupp, G.
  23. Biochem. Physiol. Pflanzen v.185 Actin polymerization as an essential process in light- and dark-controlled chloroplast anchorage Schonbohm, E.;Meyer-Wegner, J.
  24. Cell Biol. Intern. v.17 Analysis of spindle microtubule organization in untreated and taxol-treated PTK1 cells Snyder, J.A.;Mullins, J.M.
  25. J. Cell Sci. v.88 The translocation of mitochondria along insect ovarian microtubules from isolated nutritive tubes: a simple reactivated model Stebbing, H.;Hunt, C.
  26. Mycol. Res. v.95 Patterns of nuclear migration and heterokaryosis in pairings between sibling homokaryons of Heterobasidion annosum Stenlid, J.;Rayner, A.D.M.
  27. J. Cell Biol. v.119 Astral microtubules are not required for anaphase b in Saccharomyces cerevisiae Sullivan, D.S.;Huffaker, T.C.
  28. Exp. Mycol. v.18 The role of astral microtubules in conjugate division in the dikaryon of Coprinus cinereus Tanabe, S.;Kamada, T.
  29. Europ. J. Cell Biol. v.53 The role microtubules for the movement of mitochondria in pinacocytes of fresh water sponges (Spongillidae, Porifera) Weissenfels, N.;Wachtman, D.;Stockem, W.
  30. Mycologia v.86 Ultrastructure of mitosis and clamp formation in the somatic hyphae of Pleurotus ostreatus Yoon, K.S.;Kim, Y.S.
  31. Mycologia v.78 Basidiosporogenesis in Boletus rubinellus. II. Late spore development Yoon, K.S.;McLaughlin, D.J.