Korean Journal of Pharmacognosy (생약학회지)
- Volume 15 Issue 2
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- Pages.78-84
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- 1984
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- 0253-3073(pISSN)
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- 2288-9299(eISSN)
Studies on the Browning of Ixeris sonchifolia
고들빼기의 갈변에 관한 연구
- Park, Soo-Sun (College of Pharmacy, Sook Myung Women's University) ;
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Kim, An-Keun
(College of Pharmacy, Sook Myung Women's University)
- Published : 1984.06.30
Abstract
Polyphenol oxidase was purified from acetone powder extract of the root of Ixeris sonchifolia. The enzyme obtained by ammonium sulfate fractionation and sephadex G-200 gelfiltration gave 51-fold purification over the crude extract. The purified enzyme showed activity toward chlorogenic acid, caffeic acid and pyrocatechol. The kinetics of thermal inactivation of the enzyme followed first-order reaction. Potassium cyanide and cysteine were potent inhibitors.
Keywords
- Ixeris sonchifolia;
- polyphenol oxidase;
- purification;
- chlorogenic acid;
- caffeic acid;
- pyrocatechol;
- potassium cyanide;
- cysteine