• Korean Journal of Agricultural Science
• /
• v.49 no.3
• /
• pp.595-606
• /
• 2022

Industrial pig breeding has used the Duroc breed and terminal sires in a three-way crossbred system in Korea. This study identified the gene variation patterns related to carcass quality in crossbred pigs ([Landrace × Yorkshire] × Duroc) using whole-exome sequencing (WES). This study used crossbred pigs and divided them into two groups (first plus grade, n = 5; second grade, n = 5). Genomic DNA samples extracted from the loin muscles of both groups were submitted for WES. A set of validated single-nucleotide polymorphisms (SNPs: n = 102) were also subjected to the Kompetitive allele-specific polymerase chain reaction (KASP) to confirm the WES results in the loin muscles. Based on the WES, SNPs associated with meat quality were found on chromosomes 5, 10, and 15. We identified variations in three of the candidate genes, including kinesin family member 5B (KIF5B), GLI family zinc finger 2 (GLI2), and KIF26B, that were associated with meat color, marbling score, and backfat thickness. These genes were associated with meat quality and the mitogen-activated protein kinase (MAPK) and Hedgehog (Hh) signaling pathways in the crossbred pigs. These results may help clarify the mechanisms underlying high-quality meat in pigs.

• Molecules and Cells
• /
• v.40 no.8
• /
• pp.533-541
• /
• 2017

Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescribed DNA-binding mode. Due to their modular architecture and flexibility, TALEs have been rapidly developed into artificial gene targeting reagents. Here, we describe the methods used to design these DNA-binding proteins and their key applications in biomedical research.

• BMB Reports
• /
• v.40 no.2
• /
• pp.270-276
• /
• 2007

We have cloned a novel mouse zinc finger protein gene Znf313 by rapid amplification of cDNA ends (RACE) according to the homologue of human ZNF313 gene. The cDNA is 2,163 base pairs (bp) in length and encodes a 229 amino acids (aa) protein with a $C_3HC_4$ ring finger domain and three $C_2H_2$ domains. 89% and 93% nucleotide (nt) and aa sequence identity is observed with its human homologue. Revealed by Northern blot and RT-PCR, full mRNA consists of 2.16 kb and widely expresses in tissues as a single transcript, most abundantly in heart, liver, kidney and testis. The expression of Znf313 in testis is detected in all development stages. Western blot analysis also reveals that Znf313 is expressed in the tissues. Immunohistochemical staining and subcellular localization demonstrate that Znf313 is expressed both in the cytoplasm and nucleus whereas predominantly localized in the nucleus. Present data suggests that Znf313 gene might play a fundamental role in gene transcription and regulation in organism and relates to spermatogenesis.

• The Journal of Korean Academy of Prosthodontics
• /
• v.8 no.1
• /
• pp.25-29
• /
• 1968

The purposes of this study were to measure the film thickness of zinc phosphate cement between the casting restorations and preparation walls. In addition, the differences between finger press and non press techniques after the castings were seated completely on the preparation by an automatic mallet until the cement set were studied. The results were as follows : 1) In full cast crown, the cement film thickness on the side wall was thinner than that on the other walls. 2) In 3/4 crown and inlay, the cement thickness was thinner than that in cast crowns. 3) The cement of great W/P ratio showed thinner thickness than that of little W/P ratio. 4) The continuous finger press after the castings were seated completely on the preparations had few influence on the cement film thickness.

• BMB Reports
• /
• v.43 no.3
• /
• pp.193-198
• /
• 2010

In this study, we report the identification and characterization of a novel C2H2 zinc finger protein, ZNF552, from a human embryonic heart cDNA library. ZNF552 is composed of three exons and two introns and maps to chromosome 19q13.43. The cDNA of ZNF552 is 2.3 kb, encoding 407 amino acids with an amino-terminal KRAB domain and seven carboxyl-terminal C2H2 zinc finger motifs in the nucleus and cytoplasm. Northern blotting analysis indicated that a 2.3 kb transcript specific for ZNF552 was expressed in liver, lung, spleen, testis and kidney, especially with a higher level in the lung and testis in human adult tissues. Reporter gene assays showed that ZNF552 was a transcriptional repressor, and overexpression of ZNF552 in the COS-7 cells inhibited the transcriptional activities of AP-1 and SRE, which could be relieved through RNAi analysis. Deletion studies showed that the KRAB domain of ZNF552 may be involved in this inhibition.

• Proceedings of the Korean Society of Potoscience Conference
• /
• spring
• /
• pp.45-45
• /
• 2004

• Proceedings of the Zoological Society Korea Conference
• /
• 2001.10a
• /
• pp.247.1-247
• /
• 2001

No Abstract, See Full Text

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2002.11a
• /
• pp.79.2-80
• /
• 2002

• Proceedings of the Korean Society of Life Science Conference
• /
• 2003.05a
• /
• pp.147-147
• /
• 2003

• Proceedings of the Zoological Society Korea Conference
• /
• 2000.10a
• /
• pp.241.1-241
• /
• 2000

No Abstract, See Full Text