• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.58 no.2
• /
• pp.136-141
• /
• 2013

This study was conducted to investigate upland crop at reclaimed sand land 'Saemangeum' for early desalination purpose and to investigate the growth and yield of whole crop barley, which was acceded after summer crop of corn and rice. Seedling establishment of whole crop barley were 216 seedlings/$m^2$(25%) for non-flooding, 43% for 1 month and 58% for 2, 3 month flooding. And it was 60% in rice cultivation. Soil salt concentration was 0.5% in non-flooding treatment, however flooding treatments decreased to 0.2% or less. In general soil salt concentration increased until the middle stage of growing, then became to similar level as the seeding time. Plant height, stem length and number of tiller were increased with flooding treatment. Whole crop barley yield was significantly reduced in non-flooding treatment but rapidly increased by flooding treatment. Yielding at 3 months increased by 504% compared to non-flooding, and rice cultivation was also increased by 536%. Protein and fiber content was low in 1 month flooding treatment, 3 months flooding and rice cultivation showed the similar results in terms of feed value. For desalination purpose in reclaimed land, 3 months flooding treatments of rice cultivation could result in higher yielding for upland crop, such as whole crop barley.

• Journal of Environmental Science International
• /
• v.24 no.10
• /
• pp.1285-1307
• /
• 2015

We constructed marine forest to restore barren grounds which are expanding in the east coast of Korea using 2 methods of (1)seedlings transplantation method and (2)underwater floating ropes method. We transplanted 3 macroalgae species, Ecklonia cava, Undaria pinnatifida, and Saccharina. japonica to construct marine forest. Blade length of Undaria pinnatifida on underwater floating ropes was $56.70{\pm}8.69mm$ in April and grew $68.75{\pm}22.30mm$ in May and $70.75{\pm}14.36mm$ in July. Blade length of S. japonica was shown 97.95-143.00mm in April to June. Blade length of Ecklonia cava was $30.50{\pm}1.91mm$ in May, $41.55{\pm}1.84mm$ in August, $45.30{\pm}2.57mm$ in November, 2009 and $45.30{\pm}1.99mm$ in February, 2010. The survey on Dangsa area, Ulsan-city in January, 2009 found a total number of 15 algal species(1 brown algae, 14 red algae species) with the highest variety at 5m depth of A station and the lowest at 8m depth of A and B stations. The March survey showed a total of 24 species (1 green algae, 1 brown algae, 22 red algae species) with the highest variety of 11 at depths of 3m and 5m of B station and the lowest of 6 at 10m of B station. In May, total biomass was 3,755.4g (green algae 1.2g, brown algae 199.0g, red algae 3,555.2g). From January, 2009, we found that E. cava was dominant at the depths of 3m and 5m of A and B stations while Peyssonnelia capensis was dominant at the depth of 8m of A station. The 8m depth of B station was dominated by Acrosorium polyneurum. In May, Grateloupia lanceolata was dominant at 8m depth of A station while other depths were dominated by Phycodrys fimbriata. In June, the dominant species were G. lanceolata at the 3m depth, E. cava at the 5m and P. fimbriata at the depths of 8m and 10m of A station. Under B station, G. lanceolata was dominant at the depths of 3m and 5m while P. fimbriata was dominant at the depths of 8m and 10m.

• Journal of Life Science
• /
• v.18 no.8
• /
• pp.1164-1168
• /
• 2008

We investigated gravitropic responses in the primary root of maize (Zea mays) seedlings which were exposed to salt stress. The maize roots salt-stressed with higher than 100 mM NaCl or KCl started to reveal enhanced gravitropic curvature after 2 hours form the gravi-stimulation. Such a promotion was not caused by sodium phosphate, but invoked by potassium phosphate, indicating the active component is $K^{+}$. Because NaCl increased gravitropic curvature, despite that $Na^{+}$ did not played any role, we evaluated the role for $Cl^{-}$ by comparing the effects of $MgCl_2$ and $MgSO_4$. The enhancement of the curvature only with $MgCl_2$ revealed that $Cl^{-}$ played a role in the gravi-response, indicating the involvement of anion channels. These results suggest that both of $K^{+}$ and $Cl^{-}$ play roles in the regulation of osmosis that is required for cell expansion in gravitropism as well as in nyctinasty and stomatal opening.

• Journal of Plant Biotechnology
• /
• v.35 no.3
• /
• pp.235-243
• /
• 2008

Here we describe a procedure for Chinese cabbage protoplast culture and effects of various treatments. Chinese cabbage protoplasts were isolated from different parts of young seedlings as using an enzyme mixture, of which yield was maximized in seven hours around after digestion. The highest rate of initial cell division followed by micro-callus formation was obtained in the medium with 1.0 mg/L 2,4-D, 0.5 mg/L NAA, and 1.0 mg/L BA when the cotyledon-derived protoplasts were cultured. Initiation of cell division and micro-callus proliferation significantly depended upon Chinese cabbage genotype under a same culture circumstances. The micro-calli developed from cotyledon tissue of Norang-Bom cultivar successfully grew toward callus colonies on the solidified medium with 0.2 mg/L zeatin and 0.1 mM spermidine. The callus colonies generated de novo shoots at the maximum frequency of 4.3% on the medium with 5.0 mg/L BA and 1.0 mg/L NM. Our results might be helpful for further studies to enhance the regeneration efficiency in Chinese cabbage protoplast culture.

• Korean Journal of Medicinal Crop Science
• /
• v.16 no.1
• /
• pp.16-19
• /
• 2008

Shoot parts of a monocarpic and short day plant Orostachys japonicus have been used as a herbal medicine. The study was carried out to examine the effect of transplanting time (May 30, June 30, July 30) of its seedlings on its growth, morphological characters, and flowering. Two hours night-break treatment at midnight was done since Aug. 25 when samples were taken afterward every 4 weeks. Growth, morphological and flowering related characters were measured from each sample. Plant height and inflorescence length became longer with delayed transplanting time. Number of leaves including bracts and stem diameter were more and thicker when transplanted on June 30 than two other treatments. Leaves and bracts, stem, root, shoot and total dry weights showed nearly the same result to the number of leaves although floret dry weight were increased as transplanting time was delayed. Although florets were most formed when transplanted on June 30, they were not flowered because of the night-break treatment, meaning that the transplanting time had little effect on its growth and anthesis of the florets.

• Journal of Environmental Science International
• /
• v.24 no.2
• /
• pp.189-196
• /
• 2015

This study was performed to identify the effect of mixed bed soil on growth of aerial parts and root zone of daughter plants for nursery field strawberry seedling raising with expanded chaff. The plant height and leaf area of daughter plants were highest or largest in the mixed soil of ERH +RH (100:0, v/v), followed by ERH+RH (75:25). The higher the mixing ratio of RH, the shorter the plant height or the smaller the leaf area. A similar tendency was observed in fresh weight. Within a root diameter of 0-0.4 mm and a root height range of 0.4-0.8 mm, root surface area and volume were statistically significantly better with treatment of ERH+RH (100:0, v/v) compared to those of roots treated with ERH+RH (75:25), ERH+RH (50:50) and ERH+RH (25:75). The growth rate of aerial parts and root zone of daughter plants were noticeably lower in two mixing ratios of 50:50 and 25:75. According to the mixing ratios of ERH+CD surface treatment, the number of roots was greatest in plants treated with ERH+CD (80:20, v/v) and ERH+CD (85:15) on August 1. However, the number of roots was highest in plants treated with ERH+CD (85:15, v/v) on August 15. Root length was longest in the plant with no treatment, and drastically shortened from ERH+CD (90:10, v/v) in both surface and mixed treatment. Although root weight showed a significant difference in ERH+CD (90:10, v/v) treatment, its increase was gradual. The rate of root growth was highest in ERH+CD (85:15). These study findings suggest that the content ratios of mixed soil ERH+RH (75:25, v/v) or below and ERH+CD (85:15) are thought to be desirable for the production of high quality seedlings.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.59 no.2
• /
• pp.181-187
• /
• 2014

Polycyclic aromatic hydrocarbons (PAHs) are a group of ubiquitous hazardous pollutants derived from fossil fuel, various combustion sources and pyrolysis of a wide range of plastics. Because PAHs can be uptake into crop plants, the inhibitory effects on rice and soybean plants were examined in greenhouse and growth chamber experiment. Soil-applied PAHs (phenanthrene of 0, 10, 30, 100 ppm) slightly reduced the plant height and dry weight both in transplanted rice and soybean plant. The inhibitory effect on growth was greater in soybean than rice. Plant height of soybean plants treated by 100 ppm was 58.9 cm and this value was 87.2% of untreated plant. In rice plant, the plant height was less inhibited (96.0% of untreated plant) by 100 ppm at 80 days after treatment (DAT). However, leaf chlorophyll content and chlorophyll fluorescence were less inhibited by PAHs at late growth stage (after heading) although the photosynthesis-related parameters were slightly inhibited from 20 DAT to 70 DAT. In agar medium experiment with infant seedlings, inhibition of seedling length and fresh weight by phenanthrene at 100 ppm were greater as compared to the experiment with adult plant in pot. Seedling length and fresh weight were reduced by 54.2% and 33.3% for rice and 27.9% and 13.2% for soybean, respectively. The results reflected that PAHs were more inhibitory during juvenile stage than adult stage and more inhibitory to rice plant than soybean for juvenile stage.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.65 no.4
• /
• pp.426-435
• /
• 2020

To determine the damages to waxy corn caused by low temperature weather, we investigated the relationship between the temperature and duration of low temperature treatment and the changes in growth characteristics during the recovery period after the treatments in different growth periods. Growth inhibition started in the low temperature group treated at temperatures below 5℃ for three days. The inhibition ratio (IR) was more than 22% or more in all sample groups. As the treatment duration increased and the Temperature decreased, the growth was more greatly inhibited than that in the control. The IR was the highest at 27% or more in the 2nd leaf stage group treated at below 5℃ for 5 days. The IR was in the order of 2nd leaf stage > 1st leaf stage > coleoptile. The IR during recovery was the highest in the 2nd leaf stage group treated at -3℃ degree for 7 days, nd the values were 82% and 98% for NDVI and Fv/Fm, respectively. Especially, all groups treated at -3℃ showed either no changes or decreases in the growth characteristics. As a result, growth inhibition increased as the temperature decreased, and as the duration of the low temperature increased. The degree of damage was in the order of 2nd leaf stage > 1st leaf stage > coleoptile. All early seedlings stopped growing and withered when exposed to temperatures at or below -3℃ for 3 days or more.

• Journal of Life Science
• /
• v.24 no.2
• /
• pp.148-153
• /
• 2014

The objective of this study was to elucidate the effect of light-emitting diode treatment on early growth and inorganic elements in leaf lettuce (Lactuca sativa L. 'Oak Leaf'). In changes to leaf morphology, shoot elongation and hypocotyl length showed poor growth under red light irradiation, while red+blue light irradiation induced shorter plant height and more leaves, resulting in increased fresh weight. With respect to Hunter's color and SPAD values, lettuce seedlings grown under red+ blue and fluorescent light irradiation had a higher $a^*$ value but showed no other changes to SPAD values. Interestingly, redness in relative chlorophyll content was 1.4 times higher under red+blue light irradiation. Inorganic element (N, Ca, Mg, and Fe) and ascorbic acid concentrations increased in lettuce plants grown under LED light irradiation compared to those of lettuce grown under fluorescent light, which showed a higher P content. In conclusion, red+blue light irradiation, which stimulates growth and higher nutrient uptake in leaf lettuce, could be employed in containers equipped with LEDs.

• BMB Reports
• /
• v.38 no.2
• /
• pp.218-224
• /
• 2005

Antioxidant enzymes are related to the resistance to various abiotic stresses including salinity. Barley is relatively tolerant to saline stress among crop plants, but little information is available on barley antioxidant enzymes under salinity stress. We investigated temporal and spatial responses of activities and isoform profiles of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), non-specific peroxidase (POX), and glutathione reductase (GR) to saline stress in barley seedlings treated with 200 mM NaCl for 0, 1, 2, 5 days, respectively. In the control plant, hydrogen peroxide content was about 2-fold higher in the root than in the shoot. Under saline stress, hydrogen peroxide content was decreased drastically by 70% at 2 d after NaCl treatment (DAT) in the root. In the leaf, however, the content was remained unchanged by 2 DAT and increased about 14 % at 5 DAT. In general, the activities of antioxidant enzymes were increased in the root and shoot under saline stress. But the increase was more significant and consistent in the root. The activities of SOD, CAT, APX, POX, and GR were increased significantly in the root within 1 DAT, and various elevated levels were maintained by 5 DAT. Among the antioxidant enzymes, CAT activity was increased the most drastically. The significant increase in the activities of SOD, CAT, APX, POX, and GR in the NaCl-stressed barley root was highly correlated with the increased expression of the constitutive isoforms as well as the induced ones. The hydrogen peroxide content in the root was most highly correlated with the CAT activity, indicating an increased role of CAT in hydrogen peroxide detoxification under salinity stress. In addition, the results suggest the significance of temporal and spatial regulation of each antioxidant isoform in determining the competence of the antioxidant capacity under saline stress.