• Research in Plant Disease
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• v.17 no.1
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• pp.82-85
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• 2011

Bakanae disease symptom were observed in barnyard grass in paddy field in Heanam, Jeonnam. The infected plants were blighted and white mass of spore were formed on the stem. Fusarium species were isolated from infected stem and the isolates were identified as Fusarium fujikuroi based on their morphological and molecular characteristics. The isolates of F. fujikuroi were assigned to reference of F. fujikuroi among related Fusarium species based on the translation elongation factor 1-alpha gene sequence. Pathogenicity of the fungal isolates was confirmed on seedlings of rice and barnyard grass by artificial inoculation. The results indicated that barnyard grass can be inoculum source of Bakanae disease on rice. Thus, effective weed management is necessary to Bakanae disease control and healthy seed production.

• Korean Journal of Organic Agriculture
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• v.18 no.1
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• pp.55-62
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• 2010

The study was conducted to investigate effects of different organic fertilizers on 'Niitaka' pear tree performances in a controlled greenhouse. The four 'Niitaka' pear seedling trees per treatment were grown in a greenhouse for 6 months. Each pot was filled with control, rice bran (RB), coffee bran compost (CBC), and the mixture (RCC) of RB + CBC + chitin incubated compost (CIC). Each pot was filled with 1:1:1 (v/v/v) of soil : sand : fertilizer, and control, RB, CBC, CIC, and RCC were applied as treatments. Root fresh weights and root activities were increased by all the organic fertilizers, especially by RCC. RCC treated trees had the highest tree height and thickest tree trunk, and all organic fertilized trees showed increased tree growth compared to the control. The more diseased and damaged degrees on the leaf by spider mites increased leaf falling rates on all the treatments. The reduced diseased and healthy leaves were found on RCC treated trees that had better tree performance. Also, all the organic fertilized trees seemed to prevent the occurrence of disease in the leaf. Therefore, the organic fertilizer applications could be a good strategy to obtain better tree performance and maintain healthy seedlings in the nursery market or young organic pear orchard.

• Molecules and Cells
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• v.21 no.3
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• pp.418-427
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• 2006

Ionotropic glutamate receptors (iGluRs) are ligand-gated nonselective cation channels that mediate fast excitatory neurotransmission. Although homologues of the iGluRs have been identified in higher plants, their roles are largely unknown. In this work we isolated a full-length cDNA clone (RsGluR) encoding a putative glutamate receptor from small radish. An RsGluR:mGFP fusion protein was localized to the plasma membrane. In Arabidopsis thaliana overexpressing the fulllength cDNA, glutamate treatment triggered greater $Ca^{2+}$ influx in the root cells of transgenic seedlings than in those of the wild type. Transgenic plants exhibited multiple morphological changes such as necrosis at their tips and the margins of developing leaves, dwarf stature with multiple secondary inflorescences, and retarded growth, as previously observed in transgenic Arabidopsis overexpressing AtGluR3.2 [Kim et al. (2001)]. Microarray analysis showed that jasmonic acid (JA)-responsive genes including defensins and JA-biosynthetic genes were up-regulated. RsGluR overexpression also inhibited growth of a necrotic fungal pathogen Botrytis cinerea possibly due to up-regulation of the defensins. Based on these results, we suggest that RsGluR is a glutamate-gated $Ca^{2+}$ channel located in the plasma membrane of higher plants and plays a direct or indirect role in defense against pathogen infection by triggering JA biosynthesis.

• The Plant Pathology Journal
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• v.29 no.3
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• pp.305-316
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• 2013

Non-protein amino acid, ${\beta}$-amino-n-butyric acid (BABA), has been involved in diverse physiological processes including seedling growth, stress tolerance and disease resistance of many plant species. In the current study, treatment of kimchi cabbage seedlings with BABA significantly reduced primary root elongation and cotyledon development in a dose-dependent manner, which adverse effects were similar to the plant response to exogenous abscisic acid (ABA) application. BABA was synergistically contributing ABA-induced growth arrest during the early seedling development. Kimchi cabbage leaves were highly damaged and seedling growth was delayed by foliar spraying with high concentrations of BABA (10 to 20 mM). BABA played roles differentially in in vitro fungal conidial germination, mycelial growth and conidation of necrotroph Alternaria brassicicola causing black spot disease and hemibiotroph Colletotrichum higginsianum causing anthracnose. Pretreatment with BABA conferred induced resistance of the kimchi cabbage against challenges by the two different classes of fungal pathogens in a dose-dependent manner. These results suggest that BABA is involved in plant development, fungal development as well as induced fungal disease resistance of kimchi cabbage plant.

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.351-355
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• 1997

Adventitious shoots were induced from cambial tissue cultures of 3-year-old seedlings using BLG mineral salts medium supplemented with 10 mM glutamine and 30 mM sucrose. The optimum growth regulator level for bud induction was 4,5 $\mu$M BA which produced average 25.5 shoots per cambium segment. Induced buds were elongated on GD medium supplemented with 30 mM sucrose followed by LMG medium supplemented with 30 mM sucrose for further shoot elongation. Elongated shoots were rooted on half-strength GD medium containing $0.54 ;\mu\textrm{M}$ NAA with the frequency of 20%. This system proved the high morphogenic potential of cambial tissue in larch.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.61 no.1
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• pp.33-40
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• 2016

This study is focused on the evaluation of growth parameters, total polyphenol content (TPC), chlorophyll content as well as the DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity of young barley seedling (YBS) affected by elicitation. Salicylic acid (SA), methyl jasmonate (MJ), amino acid liquid fertilizer (ALF) and microbial metabolism activator (MMA) were used. Elicitation was conducted for two times and various concentrations were used in this study. The result revealed that, MJ 1 ml/L treated-YBS gave the longest seedling length of 1.33 cm, followed by the ones treated with SA 1.38 mg/L and ALF 2 ml/L, respectively. ALF 3 ml/L treatment gave the highest fresh weight of 10 seedlings, followed by MJ 5 ml/L and SA 13.8 mg/L treatment with 1.56 g, 1.55 g and 1.53 g respectively. SA 138.12 mg/L elicitor treated-YBS gave the highest Chl a, Chl b content of $8.57{\mu}g/mg$ and $3.83{\mu}g/mg$, respectively while the highest carotenoid content was found in MJ ml/L treatment with $1.62{\mu}g/mg$. Among elicitor treated-YBS, SA showed better TPC. The highest TPC was found in SA 1.38 mg/L treatment with 18.82 mg/g TAE. Likewise, SA 1.38 mg/L showed the highest DPPH free radical scavenging activity among all the treatments. However, the lowest TPC was found in ALF 1ml/L treated-YBS with 9.46 mg/g TAE, which was even lower than the control (14.31 mg/g TAE).

• Journal of Crop Science and Biotechnology
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• v.10 no.1
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• pp.45-49
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• 2007

Silicon is one of the key elements for healthy growth and development in rice crops. We analyzed the effect of silicon(Si) on some growth parameters, plant mineral contents, and bioactive gibberellins in three rice cultivars. Silicon was applied at the rates of 0 kg/0.1ha(control), 40 kg/0.1ha, and 80 kg/0.1ha throughout the course of experiment. Plant growth parameters were enhanced by the application of elevated Si, though plant height and culm length were more favorably affected than the respective dry weights. The plant mineral contents analyzed also increased in treatments where Si was applied without potassium, demonstrating that Si application promotes the absorption of these minerals in rice crops. The endogenous gibberellins measured in our study showed that $GA_1$ is the only bioactive GA form present in rice seedlings. The endogenous $GA_1$ and its precursor $GA_{20}$ contents increased after Si application. However, this increase in endogenous $GA_1$ and $GA_{20}$ contents, and plant growth parameters were different according to the rice cultivars. Our results indicate that Si is a beneficial element in rice nutrition and that different cultivars of Oryza sativa show differential responses to Si nutrition in terms of their growth and development.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.298-301
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• 2005

Potato (Solamum tuberosum 'Dejima') plantlets were investigated on culture type and initial quantity of inoculation in bioreactor and survival rate by hydroponics for mass production. rode stems (1 to 1.5cm in length) of potato plantlets multiplied in vitro were grown for 3 weeks in liquid Murashige and Skoog (MS) medium with sucrose $30 g\; L^{-1}$. When plantlets (80-node inoculation) were raised in 10L balloon type bubble (BB) bioreactor, the healthiest growth of plantlets was obtained from explants cultured in ebb & flow culture with medium supplied periodically 12 times per day. The suitable inoculation quantity of 20L BB bioreactor was 120 pieces of stem segments (mean 2.2g fresh weight) in ebb & flow culture. Number of nodal shoot was eight on the average. In controlled culture room, survival rate of plantlets at 7 days after stem cutting was above $70\%$ when they were acclimatized by hydroponics grown in deep flow and solid medium culture. The highest survival rate of the stem cutting plantlets was in nutrient solution adjusted to EC $1.4dS{\cdot}m^{-1}$. Stem cutting plantlets through one culture could be obtained $670\~900$, when plantlets were grown in ebb & flow culture during 3 weeks using a 20L bioreactor with initial 120 pieces of nodal segments. 11 is possible In do mass production of seedlings cultured in bioreactor and hydroponics.

• Korean Journal of Environmental Agriculture
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• v.16 no.3
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• pp.216-219
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• 1997

Thirty day old rice seedlings, Ilpoom and Taeback variety, grown under deficient condition of several elements in nutrition solution of sand culture were exposed to 20Gy, 40Gy, 80Gy of gamma ray $(^{60}Co)$ to study the effect of their radiosensitivity. The results obtained are summarized as follows : Plant height and fresh weight of nutrient deficient rice plants were far less than those of control. The effect was different with nutrient element and rice variety, as show increasing effect of radiosensitivity in the Ilpoom variety was high in the Fe deficient whereas that of Taeback was high in the P deficient. Growth inhibition of rice plant irradiated with 20Gy was highly occurred in the Fe and Zn deficient plot in Ilpoom variety and P and Zn deficient plot in Taeback variety. In 80Gy irradiated group, radiosensitivity of rice plant was high in the P and Ca deficient plot of both Ilpoom and Taeback variety.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.2
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• pp.72-79
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• 2010

This research was initiated to investigate the revegetation characteristics under seedling types of native woody plants on a disturbed slope. 4 different seedling types of woody plants (control, unit seedling plot, nature seedling plot, pot seedling plot) with the same woody plants (Rhus chinensis Mill, Albizzia julibrissin Duraz., Quercus acutissima Carr., Pinus densiflora S. et Z.) were treated with 3 replications on the experimental disturbed slope. Data such as the died number of native woody plants, growth height and visual quality were analyzed. The died number of plants were high in unit seedling plot when compared to the rest plots of seedling types. From the standpoint of died plant species, the died number were high in Rhus chinensis and Albizzia julibrissin whereas the died number were low in Quercus acutissima and Pinus densiflora. That's because we used 1-year old seedlings of Rhus chinensis and Albizzia julibrissin and 3-year old seedling Quercus acutissima and Pinus densiflora. This result indicated that the died reason of native woody seedling was not species but seedling age. Both growth height and visual quality were ranked first in the pot seedling plot, second in the nature seedling plot, third in the unit seedling plot, and last in the control plot, respectively. However, the difference of both growth height and visual quality in each seedling plot was not observed on the 11 months after planting. Especially, the main reason of low rooting and growth value in the unit seedling plot was due to wooven fabric outer crust of pot that obstruct seedling rooting into the soil. We concluded that pot seedling of native woody plants was effective in the viewpoint of the ecological disturbed slope revegetation.