• Journal of Microbiology and Biotechnology
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• v.26 no.7
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• pp.1303-1310
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• 2016

Although many studies on the effects of genetically modified (GM) crops on soil microorganisms have been carried out over the past decades, they have provided contradictory information, even for the same GM crop, owing to the diversity of the soil environments in which they were conducted. This inconsistency in results suggests that the effects of GM crops on soil microorganisms should be considered from many aspects. In this study, we investigated the effects of the GM drought-tolerant rice MSRB2-Bar-8, which expresses the CaMSRB2 gene, on soil microorganisms based on the culture-dependent and culture-independent methods. To this end, rhizosphere soils of GM and non-GM (IM) rice were analyzed for soil chemistry, population densities of soil microorganisms, and microbial community structure (using pyrosequencing technology) at three growth stages (seedling, tillering, and maturity). There was no significant difference in the soil chemistry between GM and non-GM rice. The microbial densities of the GM soils were found to be within the range of those of the non-GM rice. In the pyrosequencing analyses, Proteobacteria and Chloroflexi were dominant at the seedling stage, while Chloroflexi showed dominance over Proteobacteria at the maturity stage in both the GM and non-GM soils. An UPGMA dendrogram showed that the soil microbial communities were clustered by growth stage. Taken together, the results from this study suggest that the effects of MSRB2-Bar-8 cultivation on soil microorganisms are not significant.

• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.93-102
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• 2019

The materials of artificial soils in the production process of container seedlings have a great influence on plant growth. Peat moss, vermiculite, and perlite have been used as major components of artificial soils for many years; however, they could decrease carbon fixation carried out by the soil and cause environmental problems such as a change in the water quality. Thus, environmental friendly materials to replace them must be developed. The purpose of this study was to verify the optimum additive materials of artificial soils and their mixed ratios for the growth and seedling quality index (SQI) of Quercus serrata. Rice husk, mushroom sawdust, and pine bark were each used as an additive material and mixed into the growth medium at 10% and 20% of the total volume. There was no significant difference in the height growth of Q. serrata. The 20% mushroom sawdust decreased the root collar diameter by 23.4% compared to the control. The total dry weight was highest with the 10% rice husk and was significantly lower by 10.3% for the 20% mushroom sawdust compared to the control. Additionally, the SQI for all the treatments showed no tendency to increase compared with the control. Thus, this study showed the possibility of recycling biomaterials from agriculture and forest for seedling production. This method could reduce environmental problems and help eco-friendly nurseries to achieve a carbon negative impact by the recycling of by-products.

• Korean Journal of Agricultural Science
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• v.31 no.1
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• pp.1-8
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• 2004

This study was carried out to identify the effects of seaweed extracts(GA14) on growth of two rice cultivars, Junambyo and Donganbyo. Seedling qualities of two cultivars were better in all items including heading dates at early stage treated at seedling plus 2-3 leaf stages than at single treatment of seedling. Ripening ratio of Junambyo in paddy field was increased 0.3% by seaweed extracts(GA14) treatment, but that of Donganbyo decreased 0.5%. 1,000 grain weight of Junambyo and Donanbyo by seaweed extracts(GA14) treatment was two to four grams higher and the yield of two cultivars was also higher by three to four percent. Appearance characters of two rice cultivars was high in head, while damaged, chalky and crack rate were low at seaweed extracts(GA14) treatment. Protein, moisture and amylose characteristics related to table quality of Junambyo were not different by seaweed extracts treatment, but table values was high in only treatment. Donganbyo was also similar to Junambyo, but table quality was slightly high at non-treatment.

• Korean Journal of Agricultural Science
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• v.46 no.4
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• pp.781-790
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• 2019

Changes in the physical and chemical properties of soil materials during the nursing process have a great influence on the quality of containerized seedlings and on growth and survival after planting. In this study, the effect of biomaterials and their mixed ratios on the growth of Zelkova serrata seedlings in a containerized seedling production system was investigated. Mushroom sawdust, pine bark, and carbonized rice husk were used as biomaterials. The mixed ratios were 10% and 20% volume ratio of the growing medium volume, including the untreated controls. There was no significant difference in the height growth of the Zelkova serrata seedlings according to the biomaterials. The root collar diameter was the highest with the 20% carbonized rice husk and the lowest with the mushroom sawdust. The difference between the highest quality index and the lowest quality index was 30% in the order of the carbonized rice husk, pine bark, control, and mushroom sawdust, but there was no statistical significance. In this study, if the growing medium mixed with biomaterials does not reduce the seedling growth compared with the control, it is considered that the biomaterial can replace a part of the growing media. Therefore, the results show that some of the growing media can be replaced with carbonized rice husk or pine bark when producing Zelkova serrata seedlings.

• Research in Plant Disease
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• v.10 no.1
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• pp.69-72
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• 2004

The control activity of isoprothiolane and tricyclazole mixed with carbosulfan, and probenazole by the nursery treatment was performed against rice leaf and neck blast caused by Magnaporthe grisea. In the paddy field, three fungicides showed good activities against leaf blast 3 months after nursery treatment. Especially the activity of tricyclazole against leaf blast gradually increased by the laps of time to 85.5％, which was assessed at 6 September,2003. Although the control value of isoprotholane and tricyclazole mixed with carbosulfan against neck blast was 47.5％ and 61.1％, respectively, probenazole showed a very high activity against not only leaf blast but also neck blast, of which that was 91.2％. No phytotoxicity was observed in all the treatments after transplanting rice seedling in the paddy field. Based on these results, three systemic fungicides tested in this study showed such a good potential that they might be used to formulate the nursery treating granule.

• Journal of the Korea Organic Resources Recycling Association
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• v.10 no.2
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• pp.71-81
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• 2002

The study was carried out to develop nursery soil for rice seedling of phyllite. First of all, physico-chemical properties of used phyllite in the study through the analysis for agricultural utilization evaluation are as following. Bulk density(BD) of phyllite was $1.31g/cm^3$ each other and porosity had 65% of entire pore size. Also, the water holding capacity(WHC) was 43% at 1/3bar pressure, which phyllite has high WHC. According to, the results the experiments for nursery soil were conducted by mixing the materials such as phyllite, zeolite and hill soil. The mixing ratios were 30, 50, and 70% for zeolite and hill soil into phyllite. These mixed materials were packed in a box by adding 0, 1 and 2g of N-fertilizer. At seedling test, there were increases in the growth of shoot and root of rice for phyllite to zeolite and phyllite to hill soil, respectively. On the other hand, the length of leaf increased with increasing application rate of phyllite, while length and a number of root increased with increasing application rate of hill soil. The growth in the plots of phyllite to zeolite and phyllite to hill soil was better than in control plot. Finally, phyllite plot had efficient results when it compared with others and the study used with phyllite will have to more research and effort for agricultural useful material.

• Proceedings of the Korean Society of Crop Science Conference
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• 2001.09a
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• pp.98-99
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• 2001

• Research in Plant Disease
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• v.9 no.1
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• pp.52-56
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• 2003

It was conducted to investigate the effect of rice downy mildew (RDM) infection to plant growth and yield components in water seeding stage, and to screen of varietal resistance to downy mildew. Being infected by rice downy mildew, chlorotic spot appeared in the leaf and leaf length was shortened. As the infected rice was growing, internode was not elongated properly and was deformed, and then panicle was not arised or mal-formed. Plant height of infected rice was shortened at all growth stage, and while the number of tillers of infected rice was more decreased than that of healthy plant before maximum tillering stage, and that of infected rice was more increased after heading stage. While the number of internode of infected tiller was much increased than that of healthy tiller internode length of infected tiller was shorter. As the rice infected by RDM severely, the number of panicles per square meter and ripening of rice was more decreased and yield of rice was extremely much decreased. As result of the varietal resistance screening with rice seedling, Geyh-wabyeo and Donjinbyeo were resistant varieties to downy mildew.

• Korean Journal of Organic Agriculture
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• v.7 no.2
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• pp.153-161
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• 1999

No-till direct-sown rice-wheat relaying cropping system has major advantages such as labor and cost saving by eliminating tillage and preparation of seed bed and transplanting. In this system, rice sowing was done simultaneously wheat harvesting. A paddy field experiment was conducted to evaluate effects of no-till years on soil microbial changes and soil physico-chemical characteristics with rice growth and development. Chemical fertilizers and agricultrual chemicals was not applied in no-till system. As the year in no-till direct-sown system the air permeability was increased and after water submerging soluble nitrogen was released Aerobic microbial-n was highest in May and then decreased after water irrigation. The population of aerobic soil microorganisms were steeply decreased after water submerging Soil microorganisms was decreased with the increased the soil depth. A month was needed for the seedling establishment in a no-tillage rice-wheat cropping system. Increased cropping years improved leaf greenness and leaf area index(LAI). But stomatal conductance(Gc) was higher in conventional cultivation system than no-till system. Stomatal conductance at panicle initiation stage was increased higher in conventional condition of leaves but the difference between conventional and no-till system was increased at heading stage. In no-till 4 years condition rice grain yield was spikelet numbers per panicle.

• Korean Journal of Environmental Agriculture
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• v.18 no.3
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• pp.229-235
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• 1999

To identify the differences on plant growth and yield of two rice cultivars among direct water-seeding methods broadcasting on flooded paddy surface(BF), drilling on flooded paddy surface(DF), and puddled-soil drill seeding(PD) under markedly different weather condition between 1995 and 1996. The mean air temperature for duration from May to June, early growth stage of rice in 1995 was lower $1{\sim}3^{\circ}C$ than that in normal or 1996. In 1995 the respiratory consumption index during panicle formation stage and early ripening stage was higher than those of in 1996 or normal year. Number of seedling stand among the methods of direct seeding rice appeared slightly higher in order of BF>DF>PD. Properly in Nonganbyeo, the number of seedling stand was much low in 1995 compared with in 1996. The leaf area index and shoot dry weight at early growth stage of rice plant and culm length at mature in 1995 were larger in direct water seeding rice than those of machanical transplanting rice, but in 1996. Faster ripening speed and shorter ripening period of rice crop appeared in 1996 compared to in 1995. It was due to higher growing degree-days, sunshine hours and solar radiation during rice growing season in 1996. Dongjinbyeo rice showed higher yield than Nonganbyeo which had lower ripened grains especially in 1995.