• 한국축산식품학회지
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• 제23권4호
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• pp.333-341
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• 2003

오미자 물 추출액을 첨가한 드링크 타입 요구르트를 개발하기 위한 기초 연구로 오미자 물 추출액 제조를 위한 최적 추출 조건을 확립하였고, 오미자 물 추출액이 유산균의 생육에 미치는 영향을 조사하였으며 오미자 물 추출액 첨가 드링크 타입 요구르트를 저장하는 동안 유산균의 변화를 관찰하였다. 오미자의 최적 추출 조건은 오미자를 50배수의 물로 추출 온도 2$0^{\circ}C$에서 15시간 추출하는 것이 가장 적합하였으며, 추출액의 pH는 3.07, 산도는 2.39%, 당도는 1.10%, 색 흡광도는 0.15이었다. 오미자 물 추출액의 첨가 농도(0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9와 1.0%)에 따른 유산균 증식에 미치는 영향을 조사한 결과 12시간 배양에서 Lac. acidophilus가 0.9% 첨가에서도 가장 안정된 생육을 보였으며 그 다음이 Lac. casei, Lac. bulgaricus와 Str. thermophilus 순으로 각각 오미자 물 추출액 0.8%, 0.2%, 0.1% 첨가 수준에서 생육이 유지되었다. 따라서 오미자 물 추출액 첨가 드링크 타입 요구르트 제조시 Lac. acidophilus를 starter로 사용하는 것이 가장 적절하였다. 오미자 물 추출액을 0.4%, 0.6%, 0.8%, 1.0%첨가하여 제조한 드링크 타입 요구르트를 4$^{\circ}C$에서 15일 동안 저장하였을 때 15일째의 유산균수는 각각 1.77${\times}$$10^{9}$ CFU/mL, 2.29${\times}$$10^{9}$ CFU/mL, 1.27${\times}$ $10^{9}$ CFU/mL, 1.13${\times}$$10^{9}$ CFU/mL로 약간 감소하였으나 0일의 기준 시료와 큰 차이 없이 유사한 균수를 유지하였다. 이것은 축산물의 가공 기준 및 성분 규격에 나타난 농후 발효유의 법적 유산 균수(1.0${\times}$$10^{8}$ CFU/mL 이상)를 충족하는 것으로 나타났다.

• 한국축산식품학회지
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• 제26권1호
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• pp.144-147
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• 2006

발효 유제품 제조에 오디 추출물을 첨가할 때 유산균 생육에 미치는 오디 추출물의 효과를 측정하여 오디의 이용성을 넓히고자 한다. 오디 추출물은 에탄올 함량이 60%일 때 가장 높은 추출 수율을 보였다. L. casei와 L. acidophilus의 생육에 대한 영향을 측정하기 위해 오디 추출물을 1%, 5%, 10% 첨가시킨 후 MRS 배지에 유산균을 접종하여 24시간 배양후 pH, 총산도 및 생균수를 측정한 결과, 첨가량이 1%일 때 가장 낮은 pH 4.19와 4.11를 보였으며, 생균수 측정시 오디 추출물 무첨가시 $2.3{\times}10^9{\sim}4.2{\times}10^{10}\;CFU/mL$의 생균수를 보인 반면 오디 추출물 첨가시 $1.8{\times}10^9{\sim}7.8{\times}10^{10}\;CFU/mL$의 생균수를 보임에 따라 다소 높은 생균수를 보였다. 또한 총산도 역시 오디 추출물 첨가시 증가하는 경향을 보였다. L. casei와 L. acidophilus을 이용한 발효 유제품 제조시 오디 추출물을 각각 1% 첨가시 발효 유제품에 미치는 영향을 측정한 결과, 오디 추출물을 첨가한 발효 유제품의 산도 및 생균수가 첨가하지 않은 발효 유제품에 비해 높은 경향을 보였다. 오디 추출물 1% 첨가시 산도를 측정한 결과 L. acidophilus을 이용한 발효 유제품의 산도가 가장 낮은 것으로 나타났으며, 생균수 역시 $6.9{\times}10^{10}\;CFU/mL$로 가장 많은 생균수를 보였다.

• 한국식품영양학회지
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• 제15권1호
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• pp.42-50
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• 2002

Skim milk에 매실 착즙액을 수준별로 첨가하고 4종(Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei)의 젖산균을 단독균주 또는 혼합균주로 접종하여 매실 착즙액의 첨가가 젖산균의 산생성 및 생육에 미치는 영향과 저장성을 조사하였고, 매실 착즙액으로 사용한 매실의 일반 성분을 분석하였다. 매실의 일반 성분은 조회분 0.4%, 식이섬유 4.1%, 구연산 4.66%, 총당 0.264%, vitamin C 405.34mg%이었고, 매실 착즙액의 첨가는 젖산균의 증식을 촉진시켰으며 산생성도 증가하였다. 실험구 중 Str. thermophilus, Lac. acidophilus와 Lac. casei의 혼합균주에 3% 매실 착즙액을 첨가한 실험구가 가장 많은 양의 젖산(1023%)을 생성하였고, 가장 높은 생성균수(3.6$\times$$10^{11}$ cfu/mL)를 나타내었다. 매실 착즙액 3% 첨가 호상 요구르트를 대조구와 함께 4$^{\circ}C$와 2$0^{\circ}C$에서 30일 동안 저장한 결과, 세 개의 혼합균주(Str. thermophilus, Lac. acidophilus)를 사용한 호상 요구르트가 4$^{\circ}C$에서 pH는 4.25, 생균수는 4.1$\times$$10^{9}$ cfu/mL 이었고, 2$0^{\circ}C$에서 pH는 3.76, 생균수는 8.7$\times$$10^{8}$ cfu/mL으로 대조구인 매실 무첨가 호상 요구르트에 비하여 높은 저장성을 나타내었다. 이상의 결과로 보아 매실 착즙액을 첨가한 호상 요구르트의 제조가 가능할 것으로 사료된다.

• 동아시아식생활학회지
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• 제9권4호
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• pp.501-513
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• 1999

In order to investigate consumption pattern and image of beverage, this study was performed by using questionnaire based on stratified random sampling about 200 students from September 6, 1999 to September 18, 1999. The result was as followings: 1. For the reasons of each beverage consumption, coke, soda pop and sport drinks were for quenching thirst. And coffee and juices were for dessert and milk.yoghurts, dietary beverages, green tea and ginseng tea were for health. 2. For the priority in selecting each beverage, trademark was dominant factor for choosing coke. And the dominant factor of choosing milk.yoghurts and ginseng tea was nutrition and in case of other beverages taste was the first factor. 3. For the place of taking beverages, coffee, coke and sport drinks were mainly consumed in school and milk.yoghurt and juices in home. 4. The college students loved or liked milk.yoghurt and juices, and they liked or enjoyed coke, soda pop, sport drinks and green tea moderately, and enjoyed ginseng tea and black tea moderately or disliked them. 5. For the frequency of each beverage, many of them consumed coffee and milk.yoghurt once or twice a day. and consumed coke. soda pop. sport drinks and juices once or twice a week. And they answered that they did not consume dietary beverages, green tea, ginseng tea and black tea so often. 6. There was significant correlation with frequency of drinking and preference score of beverage. 7. For the consumption of the Korean traditional beverages, more than 50% of the subjects had no experience in taking sangwha tea, omiza tea, and Kugiza tea. Among them, many answered that they would try the Korean traditional beverages, if they had an opportunity, and that their price was moderate. 8. The results from the analysis of the image of those beverages were as followings; \circled1 Coffee, coke, sport drinks and dietary beverages had a modern image and green tea, ginseng tea and black tea had a traditional image. \circled2 Coke, sport drinks and ginseng tea had a manly image and juices, dietary beverages, green tea and black tea had a womanlike image. \circled3 The students recognized coffee, coke, milkㆍyoghurt, sport drinks, juices, dietary beverages and green tea to be daily and catholic. \circled4 Milkㆍyoghurt, juices, dietary beverages, green tea, ginseng tea and black tea were recognized to be good for health and coffee and coke bad for health. \circled5 Coffee, coke, soda pop, sport drinks, juices and green tea were slightly concerned with a change. \circled6 Coke, soda pop and sport drinks had a dynamic image and coffee, juices. green tea, ginseng tea and black tea had a little static image.

• Journal of Microbiology and Biotechnology
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• 제7권1호
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• pp.1-7
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• 1997

The efficient manufacture of fermented dairy products on an industrial scale requires a supply of reliable starter cultures with properties suited to desired product specifications. These cultures must be backed by relevant research and development activities. This article describes the issues involved in establishing a centre to provide starter culture R & D for a group of independent cheese manufacturing companies, and discusses a strategic approach to the management of starter cultures.

• 한국식품과학회지
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• 제25권4호
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• pp.340-344
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• 1993

현재 시판되고 있는 5개사의 농후 발효유를 수집하여 이들의 물리화학적 특성과 저장중 변화를 관찰하였다. pH는 $3.87{\sim}4.19$, 산도는 젖산량으로 $0.97{\sim}1.4%$, 점도는 $7,850{\sim}21,000Cp$ 범위였으며, 당도는 $16.2{\sim}22.2$ Brix를 나타내고 있었다. $5^{\circ}C$ 저장 중 pH 계속 강하하여 저장 16일에 pH4.1 내외에 이르렀고, 산도는 비례하여 상승하였으며 점도는 저장기간에 따라 증가하는 현상으로 보아 젖산균의 활동이 계속됨을 보여주었다. 젖산균은 초기 $10^8{\sim}10^9CFU/ml$로 규정 이상이었고 저장중 일부 증가하거나 일정수준을 유지하고 있었다.

• 한국치위생학회지
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• 제9권4호
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• pp.864-873
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• 2009

Objectives : This study was to collect data about oral health to develop preschool children' oral health program. Methods : This research was from July 20th to August 20th, 2008 with self-administrated questionnaires by post and e-mail. The response rate was 57.6%(144 of 250). Results : 1. Daycare center provided fruit(64.6%), bread(56.9%), milk(82.6%), yoghurt(47.9%). daycare center tend to provide cariogenic snacks and drinks such as bread, yoghurt. 2. 73.6% of teachers said they reward children with sweets for good behavior. 3. Activities related to oral health was done in daycare center were : children brush their teeth with fluoride toothpaste(79.9%), catering staff receive the education about nutrition(79.2%), children participate in the activities and training related to food(78.5%), using sugary food at a birthday party(77.3%), annual dental health check-up by a dentist(65.3%). 4. The percentages of guiding principles based on documentation in the topic of the oral health were : coping with a situation where a child injured his or her teeth(76.4%), annual dental health checkup by a dentist(72.2%), teachers supervise children's tooth brushing using a fluoride toothpaste(70.8%). 5. Documented guide or course for oral health includes the following; training for coping with tooth damage(76.4%), annual dental health check-up(72.2%), and coaching children to brush their teeth with toothpaste that contains fluoride(70.8%). Conclusions : It is believed that for better children's oral healthcare, childcare teacher should be given a range of in-depth oral health program. Furthermore, through detailed analysis on the documented guide of oral health, cooperation from the associated organization will be required to have the guide placed at the childcare center.

• 동아시아식생활학회지
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• 제18권1호
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• pp.95-103
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• 2008

The aim of this study was to evaluate the physicochemical, microbial, and sensory characteristics, as well as antioxidative activities of yoghurts containing spirulina powder, during storage at 4$^{\circ}C$. The pH of the yoghurts decreased until the 9th day of storage, but significantly increased by the 12th day. Total acidity also increased until the 9th day of storage, and then significantly decreased by the 12th day. Sugar content did not change significantly (approximately 18 $^{\circ}Brix$). Viscosity(cP) increased until the 6th day of storage, but had decreased at the 12th day. The viable bacterial cell counts of the control group decreased during storage. In contrast, the viable cell counts of the spirulina containing samples increased until the 9th day, and then decreased by the 12th day of storage. Antioxidant activity decreased in all groups, but the changes in antioxidant activity were lower in the spirulina groups than in the control group. According to sensory evaluations, the samples presented no significant differences during storage. Based on the above results, the optimum concentration of spirulina was determined as 0.25%. Also, the period of circulation of yoghurt containing spirulina was estimated to be 9 days at 4$^{\circ}C$.

• 한국축산식품학회지
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• 제35권5호
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• pp.683-691
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• 2015

The objective of this study was to identify the coccoidal bacteria present in 188 samples of fermented yaks’, mares’ and cows’ milk products collected from 12 different regions in Mongolia. Furthermore, we evaluated the fermentation properties of ten selected isolates of the predominant species, Streptococcus (S.) thermophiles, during the process of milk fermentation and subsequent storage of the resulting yoghurt at 4℃. Overall, 159 isolates were obtained from 188 samples using M17 agar. These isolates were presumed to be lactic acid bacteria based on their gram-positive and catalase-negative properties, and were identified to species level using 16S rRNA gene sequence analysis. These coccoid isolates were distributed in four genera and six species: Enterococcus (E.) durans, Enterococcus (E.) faecalis, Lactococcus (Lac.) subsp. lactis, Leuconostoc (Leuc.) lactis, Leuconostoc (Leuc.) mesenteroides. subsp. mesenteroides and S. thermophilus. Among these S. thermophilus was the most common species in most samples. From evaluation of the fermentation characteristics (viable counts, pH, titratable acidity [TA]) of ten selected S. thermophilus isolates we could identify four isolates (IMAU 20246, IMAU20764, IMAU20729 and IMAU20738) that were fast acid producers. IMAU20246 produced the highest concentrations of lactic acid and formic acid. These isolates have potential as starter cultures for yoghurt production.

• KSBB Journal
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• 제6권4호
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• pp.413-424
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• 1991

Bitid"bactcria를 이용한 요구르트 제조를 위하여 B. bitidwη의 우유내에서 최시 배양조간과 B. bitidum. L. acidophilus 및 Str. thermophihilus의 혼합배양시의 최적 조건을 조사하고 요구르트 제조에 적합한 조건을 조사하여 다음과 같은 결과을 얻었다. B. bitidum의 성장을 위한 탈지유아 농도는,15% 농도가 12%농도보다 산진전은 잘 되었으나 균의 생존력에서 12% 농도가 적합한 것으보 나타났다. B. bifidum 의 성장에 대한 L-cysteine.HCl, yeast extract, glucose와 BIOS 2000의 성장촉진물 침가효과는 BIOS 2000을 첨가한 것이 산 진전면과 균수 유지면에서 B. bhifidum익 성장에 큰 효과가 있었다. B. bifidum, L.acidophilus와 Str. thermophilus를 혼합하여 배양한 것이 산 진전과 균수면에서 B. bifidum 단독 배양한 것보다 좋게 나타났다. B. bifidum. L. acidophilus 와 SIr. thermophilus의 세 균주 혼합배양액을 이용한 요구르트 제조시 접송량은 5%. 배양온도는 $37^{\circ}C$와 배양시간은 8시간이 석당하였다 . 이 경우 세 균주의 비율은 B. bifidu: L. acidophilus. Str. thermophilus가 2:1:1의 비율이 균수유지와 산 진전면에서 가상 좋은 결과를 보였다.