Effect of Water Extract of Omija(Schizandra chinensis) on Growth of Yoghurt Starter

오미자(Schizandra Chinensis) 물 추출액이 Yoghurt Starter의 증식에 미치는 영향

  • 홍경현 (경원대학교 식품영양학과) ;
  • 남은숙 (경원대학교 식품영양학과) ;
  • 박신인 (경원대학교 식품영양학과)
  • Published : 2003.12.01

Abstract

The effect of water extract of Schizandra chinensis on the growth of yoghurt starter was investigated in order to manufacture the drink type yoghurt added with water extract of Schizandra chinensis. It was the most desirable extraction conditions for Schizandra chinensis to soak in 50 times of water for 15 hours at 20$^{\circ}C$. The water extract of Schizandra chinensis showed pH 3.07, 2.39% acidity, 1.10% total sugars, and 0.15 optical density for color. The water extract of Schizandra chinensis was added to MRS broth medium from 0.1% to 1.0% and the medium was fermented by 4 types of lactic acid bacteria such as Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, and Streptococcus thermophilus. The addition of water extract of Schizandra chinensis inhibited the growth of the lactic acid bacteria. The maximum addition amounts of water extract of Schizandra chinensis was 0.9% for Lac. acidophilus, 0.8% for Lac. casei, 0.2% for Lac. bulgaricus and 0.1% for Str. thermophilus in order to maintain the propagation of the lactic acid bacteria. When the drink type yoghurts added with 0.4%, 0.6%, 0.8% and 1.0% water extract of Schizandra chinensis were kept at 4$^{\circ}C$ for 15 days, it was showed that the number of lactic acid bacteria was not significantly changed during the storage. The viable cell counts of the drink type yoghurts by addition of 0.4∼l.0% of water extract of Schizandra chinensis were 1.13${\times}$10$\^$9/∼2.29${\times}$10$\^$9/ CFU/mL, and these bacterial counts were still more than the legal standard(1.0${\times}$10$\^$8/ CFU/mL) even at 15 days of storage.

오미자 물 추출액을 첨가한 드링크 타입 요구르트를 개발하기 위한 기초 연구로 오미자 물 추출액 제조를 위한 최적 추출 조건을 확립하였고, 오미자 물 추출액이 유산균의 생육에 미치는 영향을 조사하였으며 오미자 물 추출액 첨가 드링크 타입 요구르트를 저장하는 동안 유산균의 변화를 관찰하였다. 오미자의 최적 추출 조건은 오미자를 50배수의 물로 추출 온도 2$0^{\circ}C$에서 15시간 추출하는 것이 가장 적합하였으며, 추출액의 pH는 3.07, 산도는 2.39%, 당도는 1.10%, 색 흡광도는 0.15이었다. 오미자 물 추출액의 첨가 농도(0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9와 1.0%)에 따른 유산균 증식에 미치는 영향을 조사한 결과 12시간 배양에서 Lac. acidophilus가 0.9% 첨가에서도 가장 안정된 생육을 보였으며 그 다음이 Lac. casei, Lac. bulgaricus와 Str. thermophilus 순으로 각각 오미자 물 추출액 0.8%, 0.2%, 0.1% 첨가 수준에서 생육이 유지되었다. 따라서 오미자 물 추출액 첨가 드링크 타입 요구르트 제조시 Lac. acidophilus를 starter로 사용하는 것이 가장 적절하였다. 오미자 물 추출액을 0.4%, 0.6%, 0.8%, 1.0%첨가하여 제조한 드링크 타입 요구르트를 4$^{\circ}C$에서 15일 동안 저장하였을 때 15일째의 유산균수는 각각 1.77${\times}$$10^{9}$ CFU/mL, 2.29${\times}$$10^{9}$ CFU/mL, 1.27${\times}$ $10^{9}$ CFU/mL, 1.13${\times}$$10^{9}$ CFU/mL로 약간 감소하였으나 0일의 기준 시료와 큰 차이 없이 유사한 균수를 유지하였다. 이것은 축산물의 가공 기준 및 성분 규격에 나타난 농후 발효유의 법적 유산 균수(1.0${\times}$$10^{8}$ CFU/mL 이상)를 충족하는 것으로 나타났다.

Keywords

References

  1. Choi, J. T., Joo, H. K., and Lee, S. K. (1995) The effect of Schizandrae fructus extract on alcohol fermentation and enzyme activities of Saccharomyces cerevisiae. Agri. Chem. Biotechnol. 38, 278-282
  2. Chung, H. J. (1999) Antioxidative effect of ethanolic extracts of some tea materials on red pepper seed oil. J. Kor. Soc. Food Sci. Nutr. 28, 1316-1320
  3. Do, J. R., Kim, S. B., Park, Y. H., Park, Y. B., and Kim, D. S. (1993) The nitrite-scavenging effects by the com-ponent of traditional tea materials. Kor. J. Food Sci. Technol. 25, 530-534
  4. Ha, D. M. (1998) Food Microbiology. Shin Kwang Publishing, pp. 257-258
  5. Im, K. S. (2003) Effect of fermented milk on human health. Kor. J. Food Nutr. 16, 93-103
  6. Ji, W. D., Jeong, M. S., Chung, H. C., Choi, U. K., Jeong, W. H., Kwoen, D. J., Kim, S. Y., and Chung, Y. G. (2001) Growth inhibition of water extract of Schizandra chinensis Bullion on the bacteria. J. Food Hyg. Safety 16, 89-95
  7. Jung, G. T., Ju, I. O, Choi, J. S., and Hong, J. S. (2000) The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis Ruprecht(Omija) seed. Kor. J. Food Sci. Technol. 32, 928-935
  8. Kang, K. C., Park, J. H., Baek, S. B., Jhin, H. S., and Rhee, K. S. (1992) Optimization of beverage preparation from Schizandra chinensis Baillon by response surface methodology. Kor. J. Food Sci. Technol. 24, 74-81
  9. Kim, J. E. and Chun, H. J. (1990) A study on making je-lly with Omija extract. Kor. J. Soc. Food Sci. 6, 17-24
  10. Kim, Y. M., Kim, D. H., and Yum, C. A. (1991) Changes in flavor component of Omija, Shizandra chinensis Baillon, with various extraction times. Kor. J. Soc. Food Sci. 1, 27-34
  11. Kwon, J., Lee, S. J., So, J. N., and Oh, C. H. (2001) Effects of Schizandra chinensis fructus on the immu-noregulatory action and apoptosis of L1210 cells. Kor. J. Food Sci. Technol. 33, 384-388
  12. Lee, H. J., Suh, D. S., Shin, Y. K., Goh, J. S- and Kwak, H. S. (1992) Changes of quality m stirred yoghurt during storage at various conditions of temperature and shaking. Kor. J. Food Sci. Technol. 24, 353-360
  13. Lee, J. J., Kim, H. Y., Shin, J. G., and Baek, Y. J. (1991) Studies on the changes of the physical properties and the shelf-life of the liquid yoghurt stored at different temperatures. Kor. J. Dairy Sci. 13, 124-131
  14. Lee, J. S. and Lee, S. W. (1991) The studies of com-position of fatty acids and antioxidant activities in parts of 0mija(Schizandra chinensis Baillon). Kor. J. Dietary Culture 6, 147-153
  15. Lee, J. Y., Min, Y. K., and Kim, H. Y. (2001) Isolation of antimicrobial substance from Schizandra chinensis Baillon and antimicrobial effect. Kor. J. Food Sci. Te-chnol. 33, 389-394
  16. Lee, S. H. and Im, Y. S. (1997) Effect of Omija (Schizandra chinensis) extract on the growth of lactic acid bacteria isolatd from kimchi. Kor. J. Appl. Microbiol. Biotechnol. 25, 224-228
  17. Lee, S. H. and Lim, Y. S. (1997) Antimicrobial effects of Schizandra chinensis extract against Listeria monocyto-genes. Kor. J. Appl. Microbiol. Biotechnol. 25, 442- 447
  18. Lee, S. H. and Lim Y. S. (1998) Antimicrobial effects of Schizandra chinensis extract on pathogenic microorga-nism. J. Kor. Soc. Food Sci. Nutr. 27, 239-243
  19. Mok, C., Song, K., Lee, S., Na, Y., Park, J. H., Kwon, Y. A., and Lee, S. J. (2001) Optimization of roasting process as pre-treatment for extraction of Omija(Schi-zandra chinensis Baillon). Kor. J. Food Sci. Technol. 33, 333-337
  20. Tamine, A. Y. and Robinson, R. K. (1985) Yoghurt, Science and Technology. Pergamon Press, Oxford, pp. 241-243
  21. Vanderzant, C. H. and Splittstoesser, D. F. (1992) Com-pendium of Methods for the Microbiological Examination of Foods, 3rd ed., American Health Association, pp. 80-81