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Safflower (Carthamus tinctorius L.) Anthracnose Casued by Colletotrichum acutatum in Korea (Colletotrichum acutatum에 관한 의한 탄저병)

  • 권진혁;강수웅;김희규;박창석
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.651-653
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    • 1998
  • A destructive anthracnose of safflower has severely occurred at Kyeongsangnam-do Agricultural Research and Extention Services in 1998. Incidence of the disease surveyed at 12 fields in Chinju, Sanchong and Hamyang was ranged fro 55.7 to 85.4%. Small irregular yellowish to brown spots appeared on all parts of the plant at initial infection stage and the spots enlarged or united into larger irregular to cylindrical black lesions with hollows. Above portions of infected stems were often blighted and pink colored conidial masses were colonized on the lesions. The causal fungus consistently isolated from the infected tissues were identified as Colletotrichum acutatum by following characteristics. The fungus grew well on PDA at 15~3$0^{\circ}C$ developing apricot to salmon color. Conidia were single-celled, colorless, fusiform and bisectioned and measured as 8~18$\times$3~5 ${\mu}{\textrm}{m}$. Brownish appressoria were cylindrical or ovate and sized as 5~10$\times$4~8 ${\mu}{\textrm}{m}$. Optimum temperature for growth and germination of conidia was recorded at $25^{\circ}C$ and at least 6 hours were required for infection to the host plants. Over 90% conidia were germinated and all plants were infected by artificial inoculation by 24 hours at $25^{\circ}C$. This is the first report of safflower anthracnose caused by C. acutatum in Korea.

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Delayed Clinical Symptoms of Gallbladder Rupture by Gallbladder Mucocele in a Dog (담낭 점액낭종에 의한 담낭 파열의 만성 경과 증례)

  • Noh, Daji;Kwon, Youngsam;Choi, Hojung;Lee, Youngwon;Lee, Kija
    • Journal of Veterinary Clinics
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    • v.33 no.1
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    • pp.58-61
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    • 2016
  • An 11-year-old, intact male Cocker Spaniel dog was presented with history of abdominal distension, dyspnea for 10 days and lethargy for 1 day. Abdominal radiographs showed decreased serosal detail with abdominal distension. Abdominal ultrasounds revealed gallbladder mucocele with generalized peritonitis showing stellate-like sludge in the gallbladder with echogenic fat degeneration of cranial abdomen and abdominal free fluid containing echogenic materials. Loss of gallbladder wall integrity was shown clearly on computed tomography but ambiguously on ultrasound. Ultrasound-guided abdominocentesis was performed and showed amount of yellowish-bloody peritoneal fluid with vegetable matter and mucoid substance. On peritoneal fluid analysis, bilirubin level was elevated over three times than those of the serum. On exploratory laparotomy, gallbladder rupture and generalized bile peritonitis with intestinal adhesions were confirmed and cholecystectomy with peritoneal lavage was performed. One day after operation, patient died. This report describes delayed clinical symptoms of gallbladder rupture by gallbladder mucocele. In addition, this is the first case report using computed tomography made a diagnosis gallbladder rupture in a dog. Computed tomography might be helpful to diagnose gallbladder rupture.

Four cases of gastric submucosal mass suspected as anisakiasis

  • KIM Seoung-Gu;JO Yun-Ju;PARK Young-Sook;KIM Sung-Hwan;SONG Moon-Hee;LEE Han-Hyo;KIM Jeong-Seon;RYOU Ji-Won;JOO Jong-Eun;KIM Dong-Hoon
    • Parasites, Hosts and Diseases
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    • v.44 no.1 s.137
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    • pp.81-86
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    • 2006
  • Anisakiasis is a parasitic disease caused by ingestion of raw fish infected with anisakid larvae. Endoscopic changing patterns of submucosal lesions in chronic gastric anisakiasis have not been known yet. Here we report 4 cases of suspected gastric anisakiasis which were improved during follow-up periods without surgical treatment. The patients presented with abdominal pain, nausea and vomiting after consuming raw marine fish, and visited our gastroenterology outpatient department. Their endoscopic findings showed firm and yellowish submucosal masses accompanied with eccentric erosions. Histologic findings showed severe eosinophilic infiltrations. In blood tests, peripheral eosinophil counts and total IgE levels were elevated. We believed that all cases were caused by larval anisakid infections. The submucosal mass lesions disappeared during the follow-up periods of 2 to 4 mo.

Ethylene glycol toxicosis in a dog (개의 ethylene glycol 중독)

  • Jean, Young-Hwa;Woo, Gye-Hyeong;Kim, Jae-Hoon;Hwang, Eui-Kyung;Seo, Il-Bok
    • Korean Journal of Veterinary Pathology
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    • v.2 no.2
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    • pp.147-151
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    • 1998
  • A five-month old female Great Dane with a history of depression and convulsion was submitted to the Pathology and Diagnosis Reference Division of National Veterinary Research and Quarantine service. The dog had ingested concentrated antifreeze and died 12 days after the sudden onset of clinical signs. Main gross lesion was limited to swollen kidneys with diffusely fine granularity on the cut surface. Principle microscopic lesions were focused on the kidneys including dilated Bowman's space, thickened glomerular basement membrane, many light yellowish crystals, and proteinaceous casts in some tubules. Some neutrophils and lymphocytes were focally infiltrated in interstitium of the cortex and medulla. Crystals were angular or quite irregular in shape and arranged in sheave or rosette form. Under polarized light, the crystals were birefringent, which is typical of calcium oxalate crystals. Crystals were more concentrated in the cortex than in the medulla. A few crystals were also observed in the small blood vessels of the brain. Based upon the history of ingestion of antifreeze and the identification of calcium oxalate crystals in the tissue sections of the kidney and brain, this case was diagnosed as ethylene glycol toxicosis in a dog.

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Detection of viral-like particles in feces of Korean indigenous calves with diarrhea by negative staining (음성대조 염색법을 이용한 설사 한우송아지 분변내 바이러스양 입자 검색)

  • 임종수;강춘원;이태욱;김내영;정용운;강문일;한동운;최현성;이채용
    • Korean Journal of Veterinary Service
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    • v.22 no.1
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    • pp.43-52
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    • 1999
  • Presently, viral isolation in the diarrheal feces can be reached by many tools such as fluorescent antibody test(FA), negative contrast electron microscopy(NCEM), virus neutralization test, cell culture, and so on. The purpose of the study was to aimed at the establishment of simplified NCEM technique which can be efficiently applied for diarrheal feces and also the understanding on prevalence of viral-induced diarrhea in calves. One hundred fourty-seven korean indigenous calves with diarrhea were examined to their feces by the modified NCEM. Among them, 98(66.7%) were confirmed to have one or more viruses in feces. The viruses detected were identified as rotavirus(33.3%), coronavirus(16.3% ), togavirus(10.2%) and herpesvirus(0.7%). Ten cases of combined viral infection were consisted of 8 with rotavirus+coronavirus, one with rotavirus+togavlrus and one with rotavirus+herpesvirus. Dirrheal types could classified by yello-wish watery(44.9a ), blood-tinged(19.7% ), white watery(17.7% ) , brownish watery(14.3%), greenish watery(3.4%) diarrhea, respectively. Yellowish watery diarrhea(66cases) was frequently included rotavirus(31.8%), coronavirus(15.2%), and togavirus(13.6%), respectively. Consequently, these results suggest that the modified NCEM is reliable and efficient diagnostic tool for detection of viruses in the diarrheal feces and many calves rearing in Chonnam province have been exposed to some enteric viral agents mainly including rotavirus and coronavirus.

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Spontaneous pyometra induced by Staphylococcus aureus in a Maltese (황색포도상구균 감염에 의한 어린 말티스의 자궁축농증 증례)

  • Hong, Sun-Hwa;Chung, Yung-Ho;Han, Sang-Jun;Kim, Ok-Jin
    • Korean Journal of Veterinary Service
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    • v.35 no.1
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    • pp.63-67
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    • 2012
  • An 8-months-old young Maltese bitch was presented for investigation of anorexia and occasional vomiting. The body weight was 2 kg. Abdominal sonography demonstrated abnormal enlargement of uterus. The dog had been clinically and pathologically examined. Also, ovariohystectomy was performed. Staphylococcus aureus was identified in the pus-like materials of the uterine lumen. This case was confirmed as pyometra induced by Staphylococcus aureus. Gross examination of the dissected uterus revealed enlarged uterine horn, which had yellowish or brownish pus-like materials in the lumen. Also, there was several segments formation between the enlarged areas of left and right uterine horns. Histopathologically, endometritis and necrosis were present in uterine wall. The bitch made a complete recovery following an ovariohystectomy. This case was spontaneous pyometra induced by Staphylococcus species. On our knowledge, this is a very rare report of pyometra occurrence in young dog and Staphylococcus species-induced pyometra. This study may provide a valuable data on the study of canine pyometra.

Studies on Normal Synovial Fluid from Tibiotarsal Joints of Korean Cattle 1. Gross Appearance and Cellular Constituents (한우(韓牛)의 관절골액(關節滑液)에 관한 연구(硏究) 1. 경부관절골액(脛부關節滑液)의 성상(性狀)과 세포조성(細胞組成)에 관하여)

  • Han, Hong Ryul
    • Korean Journal of Veterinary Research
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    • v.16 no.2
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    • pp.179-185
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    • 1976
  • The gross appearance, cellular constituents, and quality of mucin clot of synovial fluid from the normal tibiotarsal joints of 57 healthy Korean cattle were investigated. The results obtained were summarized as followings. 1. The gross appearance of the synovial fluid were quite clear without any minute particles of flocculent, colourless (78.95%) or slightly yellowish (21.05%), and it did not coagulate within the 24 hours after sampling. 2. The mean number of leucocytes for the entire group was $127.68{\pm}13.84/mm^3$ (SE) with a range of 0 to $627/mm^3$ for individual samples. In the total cell count no significance among the investigated age and sex categories was found. 3. The mean differential values for the groups ware: neutrophil, $9.36{\pm}1.67%$; lymphocyte, $57.66{\pm}8.34%$; monocyte, $24.70{\pm}2.22%$; eosinophil, $1.45{\pm}0.27%$; and macrophage, $5.83{\pm}1.13%$. No basophils were observed in stained smears of synovial fluid. 4. Normal mucin clot quality was recorded for 94.7% of the animals, but 5.3% were observed to have fair mucin clot quality. Quality of mucin clot was approximately equal for bulls, cows, and calves.

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New Data on the Synthetic $Pt_{34}Sb_7$ and $Pt_3Sb$ Phases (합성화합물 $Pt_{34}Sb_7$$Pt_3Sb$에 대한 새로운 자료)

  • 김원사
    • Journal of the Mineralogical Society of Korea
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    • v.1 no.1
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    • pp.15-19
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    • 1988
  • In the process of re-checking the phase relations of the Pt-Sb system the previously reported Pt4+Sb phase did not occur. Instead, Pt34Sb7 phase with an average chemical composition (wt%) Pt 89.3, Sb 11.4, total 100.7, has been newly found to exist, and the formula Pt34Sb7 has been tentatively assigned to it, It is pale brownish grey to yellowish grey under refloecting microscope and non-bireflectant, VHN100=225 (206-243). X-ray powder patterns are (d(I/Io)): 2.318(100), 2.293(100), 2.110(30), 1.440(50), 1.390(15), 1.283(5), 1.203(70) and 1.192(40)$\AA$. They are indexable on the basis of tertragonal cell with $\alpha$=3.948(3), c=16.85(1)$\AA$. A question whether the tetragonal Pt34Sb7 is the new phase or a polymorph of the cubic Pt4+Sb phase remains unclear and awaits better X-ray diffraction, electron microprobe analysis and DTA for the Pt4+Sb phase. The Pt3Sb phase reported to be of tetragonal symmetry has been confirmed to exist. It is greyish yellow in air and in oil, very weakly bireflectant, and weakly anisotropic. VHN25=216 (183-240). Its X-ray powder data have been successively indexed on a tetragonal cell with $\alpha$=3.9455(7), c=16.959(5)$\AA$.

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Identification of Buza by Detecting Aconitine-type Alkaloids (Aconitine 류 알칼로이드의 검출에 의한 부자류 생약의 확인)

  • Eom, Dong-Ok;Ban, Tae-Hwan
    • Analytical Science and Technology
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    • v.12 no.3
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    • pp.243-247
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    • 1999
  • After aconitine-type alkaloids have been isolated from the crude drug "Buza" as the precipitate, they could be identified with colour test, UV/Vis. absorption wavelength, TLC. The presence of aconitine-type alkaloids are confirmed by the reaction with the Reineke ion, $[Cr(NH_3)_2(SCN)_4]^{1+}$, and TCR ion, $[Co(SCN)4]^{2-}$, to produce the white precipitate or one of the containing from blue to yellowish blue. It is based on the formation of complex compounds by aconitine-type alkaloids with Reineke and TCR reagent. The method has been found to be simple, convenient and suitable for routine identification of aconitine-type alkaloids, related basic compounds, the crude drug "Buza" processed from the roots of certain Aconitum spp. plants(Ranunculaceae).

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Manufacture Technology Development of Paper Mending Tape for Conservation of Archive Document (종이 기록물 보수용 안전 테이프 시제품 제조 기술 연구)

  • Shin, Joung-Soon;Yoo, Sun-Kyun
    • Journal of the Korean Graphic Arts Communication Society
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    • v.29 no.3
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    • pp.41-53
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    • 2011
  • For manufacturing the tape for repairing archival documents, we tested ten carrier candidates for selecting best material. The tensile strength, transmissivity, stability of deterioration, and processability were determined. Physical-chemical characteristics and stability of deterioration was best to the Hanji. Tensile strength and transmissivity was best in tracing paper, but was low in oil paper, white sketch paper, lyon coat paper. Synthetic carrier to tensile strength showed higher than paper carrier and to the transmissivity showed 2-8 times higher than paper carriers. The tracing paper to the transmissivity was 10 times higher than others. To determine characteristic of conservation to the selected carriers, stability of deterioration was examined at conditions of $90^{\circ}C$ for 15 days. Oil paper and white sketch paper turned strongly yellowish. Cellulopane, Felt, and Cpp film showed stable stability of deterioration, but deformation like wrinkles. PET film and Syntheletic film showed excellent conservation characteristics without any change of exterior. Test of adhesive uniform between carriers and adhesive processability was performed. PET film > Syntheletic film > Tracing Paper in order were determined. Ununiformal adhesive characteristics appeared to Hangi. Accordingly, we thought that Hanji as carrier material might be unsuitable because of low adhesive processability.