• 한국산림과학회지
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• 제81권4호
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• pp.357-362
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• 1992

아까시나무와 산돌배나무의 수분이동(水分移動) 경로(經路)를 이해하고, 수분이동(水分移動) 경로(經路)를 이용한 무늬목을 생산하기 위하여, 염료침투경로(染料浸透經路)를 0.5% acidic fuchasin 용액과 0.5% fast green 용액을 수목(樹木)에 주입시켰다. 환공재(環孔材)이면서 타이로시스를 갖고있는 아까시나무와 산공재(散孔材)이면서 목섬유(木纖維)에 많은 벽공(壁孔)이 존재하는 산돌배나무의 목부구조(木部構造)와 수분이동(水分移動) 경로(經路)를 비교했다. 그 결과 아까시나무의 수분통도(水分通導)는 당해년도(當該年度)와 전년도(前年度)에 형성된 조재부(早材部)의 대도관(大導管)에 의해서 주도되었다. 그리고 타이로시스가 도관(導管) 내강(內腔)을 폐쇄(閉鎖)하고 있는 심재(心材)와 이행재(移行材)에는 염료침투(染料浸透)가 되지 않았다. 산돌배나무는 수(髓)를 제외하고 전부위에 염색되었으며, 특히 도관(導管)이외에 목섬유(木纖維)의 벽(壁)에 염료(染料)의 침착이 이루어졌다.

• Journal of Plant Biology
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• 제3권2호
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• pp.29-34
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• 1960

From the view point of phytopathological anatomy, the author has tried to study the effect of the shoot cluster disease virus on the internal structure of vascular tissues of chinese date tree (Ziziphus jujuba var. inermis Rehd.) comparing healthy checks and diseased plants. The materials were collected at the several sites, Kumgock-Ri, Masuc-Ri, Kyungi-Do, and near the campus of Korea University and around the area of Chongam-Dong, Seoul City, from August 15th to September 5th 1959. The leaf materials of healthy and diseased plants are fixed and aspirated in two kinds of killing solutions, formalin-acetic acid alcohol solution and Craf III solution. Sections were cut at 5-10$\mu$ thickness and stained with the double staining reagents of safranin and fast green. In this experiment the author has observed that there are marked structural changes in the infected plants in contrast of healthy checks. As figures 3-7 show that the following characteric changes have taken place on infected plants: 1) the arrangement of irregularly developed sieve elements in phloem, 2) the degeneration of phloem elements, 3) the irregular arrangement of epidermis in mid-vein, 4) more necrosis is observed among the parenchymatous cells, 5) abundant accumulatin of starch grains in parenchymatous cells, . In contrast to the above irregularities caused by the virus disease, the healthy checks appear normal structures as shown in figures 1 and 2. In adding to the all features noted above, the author could also observe an interesting feature that the xylem elements in mid-vein vascular bundle tissues are considerably disorganized to show the unspecialized vessel elements, the irregularly arranged xylem elements. However, this kind of irregularities which occur in xylem under the virus infection has not been reported previously. The features noted on the internal structure of vascular bundle under the condition of infection by the shoot cluster disease on chinese date trees appear to be more or less closely similar to the symptoms of the bunchy-top of banana and the yellow dwarf disease of barley in respect to the fact that whether phloem necrosis takes place as a primary symptom or a secondary symptom. In all these disease, primary histological changes of hypoplasia and hypertrophy are preceeded by the necrosis of phloem.

• 한국응용곤충학회지
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• 제23권4호
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• pp.215-220
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• 1984

Dienes 염색법을 국내에 발생하는 마이코플라스마성 식물병의 진단에 적용해 보면서 본 염색법의 보다 효과적인 이용을 위한 실험에서 얻어진 결과를 요약하면 다음과 같다. 1 Dienes 염색법을을 통한 식물 마이코플라스마병의 진단방법은 일일초와 뽕나무에서만 지정적이었고, 대추나무, 오동나무 및 붉나무에서는 부정적이었다. 2. Dienes 염색액의 $0.2\%$와 $0.4\%$ 수용액이 일일초와 뽕나무에 각각 적합했으며, 염색시간은 $5\~10$분 그리고 탈색시간은 $10\~20$분이 좋았다. 3. 정확한 현미경적 관찰이나 선명한 사진을 위해서 절편의 두께는 $50\~100{\mu}$가 적당하였다. 4. 공시식물의 각 부위중에서 줄기 정부 바로 밑의 부위가 좋은 결과를 나타냈으며, 엽병이나 중륵도 거의 동등한 결과를 나타냈다. 5. 이병식물에서 염색반응의 정도는 병세와 비례했으며, 병징발견 초기의 시료는 건전시료와 염색반응에 있어서 쉽게 구별되지 않았다. 6. 시료를 종절할 경우 대부분의 관요소들이 상처를 받지 않을 뿐만 아니라 정확한 관찰이 용역하므로 시료를 횡절할 경우보다 효과적이었다. 7. 염색된 시료를 현미경 관찰할 경우 filter를 사용하지 않고 텅스텐 적색광을 그대로 사용하면 사관요소와 도관요소 간의 색갈 대비를 증가시키며 정확한 해석에 도움을 주었다. 8. 시료를 고정액에 고정시키면 염색반응이 방해되었으며, 시료는 신선한 것일수록 염색반응이 뚜렷하였다.

• 대한본초학회지
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• 제30권4호
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• pp.65-69
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• 2015

Objectives : The root ofCyanchum wifordii(CW) had been used as herbal medicine, 'Baeksuo', in Korea. However, the root ofCynanchum auriculatum(CA) had also been mis-used as 'Baeksuo' in herbal markets, due to its morphological similarity with CW. This study aimed to compare discriminative features and establish the identification-keys between two herbal medicines using microscopic methods.Methods : Microscopic preparations including fixation, rapid dehydration, paraffin infiltration, paraffin embedding, sectioning, mounting, and staining, were performed and the microscopic features between CW and CA samples were compared.Results : The starch grain of parenchymal cells, the amounts of calcium oxalate crystals, cell sizes, and the shape of phloem were not different between CW and CA samples. However, the clusters of stone cells which were conglomerated at outer cortex were apparently observed in CA sample (4~8 layers of stone cells), but not in CW sample (2~4 layers of stone cells, or not). Xylem of CW consisted of several vessels and fiber bundles which were formed as linear shapes and discontinuous radial lines, while that of CA, in which radial lines was not observed, were shaped as a wedge and scattered near cambium with comparatively few fiber bundles enclosed vessels. Especially, in the xylem of CA, fiber bundles located towards cambium were relatively more developed than those located outward cambium.Conclusions : The identification-keys established in this study would be helpful to discriminate microscopic features between CW and CA.

• Journal of Ginseng Research
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• 제28권3호
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• pp.149-156
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• 2004

The objectives of this study were to investigate the morphology of mycorrhizal roots, and the effects of root age and soil texture on the mycorrhizal infection in ginseng (Panax ginseng C. A. Meyer) growing in Korea. Ginseng roots at ages of two to six years were collected from fields in late June. Their infection by arbuscular mycorrhizal fungi(AMF) was studied by clearing the roots and staining fungal hyphae with trypan blue. Root infection varied greatly depending on the developmental stages of young roots. Young tertiary roots, in diameter of smaller than 0.8 mrn, formed during the current growing season had root hairs and were frequently and in some cases heavily infected by AMF. Hyphal coils and arbuscules were abundant, while vesicles were rarely observed. Older secondary or tertiary roots in diameter of bigger than 1.0 mm with fully differentiated primary xylem formed during the previous growing season had no root hairs, and were not infected at all. The rates of mycorrhizal infection in the young tertiary roots were not affected by the age of the ginseng plants, suggesting that fungal populations might have not much changed during the aging of the cultivated fields up to six years. The differences in the infection rates among the different ages of ginseng were caused by differences in the amount of young tertiary roots in the samples. Soil texture, either sandy loam or clay loam, did not affect the rate of root infection. There were large variations in the infection rates among the different farms and locations within a farm. It strongly suggested that infection rates of the ginseng roots by AMF would be influenced by the practice of the farmers, possibly by avoiding consecutive planting, introduction of new topsoil, and the ways of handling the soil before transplanting the ginseng, such as fumigation or sterilization that might have affected indigenous inoculum sources of the AMF.

• 대한본초학회지
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• 제26권1호
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• pp.1-6
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• 2011

Objectives : Due to morphological similarity, many substitutes and counterfeits have been used as Moktong for thousands of years. This study was designed to determine discriminative criteria for identifying medicinal herbs used as Moktong. Methods : Akebia quinata, A. trifoliata, Clematis armandii, and Aristolochia manshuriensis were collected in various regions. Samples were authenticated and examined by observation and microscopy with paraffin embedding and triple staining using the modified Ju staining method. Results : Three different types of features to establish discriminative criteria for four kinds of Moktong were identified. Botanical features include differences in phyllotaxy, leaf shape, and caulescent. External morphological features were arrangement and size of fine holes(xylem), and overall shape of the section. Internal morphological features include the number of medullary rays, shape of the pitch, type of tissues rounding pitch, appearance of annual rings, shape and amount of crystals(calcium-oxalate), and the appearance of cork cambium. Further details(e.g. identification keys) are in the article. Conclusions : These criteria could provide an effective method for identifying numerous kinds of Moktong distributed in markets throughout northeast Asian nations.

• Journal of the Korean Wood Science and Technology
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• 제36권4호
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• pp.84-92
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• 2008

강원도 양양 지역 산불 피해 소나무 목재의 피해 정도에 따른 해부 및 물리적 특성을 조사하였다. 피해 정도에 따라 수피의 탄화 깊이가 깊어지는 경향이 관찰되었으나 목질부까지 탄화가 진행되지는 않았으며 수지의 삼출정도에 차이를 보였다. 산불 피해목 변재의 생재함수율은 건전재보다 다소 낮게 나타났으나 심재의 경우에는 건전재보다 피해목이 다소 높게 나타났다. 생재밀도는 피해재 심재가 건전재 심재보다 다소 높게 나타났으며, 변재에서는 차이를 보이지 않았다. 형성층대를 관찰한 결과, 산불 피해를 강하게 입은 경우 형성층의 활동이 일어나지 않았고, 비교적 피해를 약하게 입은 경우에는 형성층의 활동이 관찰되었으나 세포분열 속도는 건전재보다 느렸다. 산불 피해를 강하게 입은 목재에서는 에피델리움세포와 방사조직의 세포벽이 짙게 염색되고 세포내에 과립형태의 물질이 관찰되었으며, 최외각 연륜 부위의 결정화도는 산불 피해재가 건전재보다 다소 높게 나타났다.