• Journal of Korean Society of Forest Science
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• v.81 no.4
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• pp.357-362
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• 1992

To understand water flow path in the cells of Robinia pseudoacacia and Pyrus ussuriensis and obtain color wood from them, this experiment was performed by penetrating 0.5% acidic fuchsin and 0.5% fast green solution into the living wood of them. A comparison was made of the wood structure and water flow path from Robinia pseudoacacia containing the ring-porous wood with tyloses, and Pyrus ussuriensis including solitary diffuse-porous wood with distinct fiber pits. The dye penetrated into vessel elements in the early wood of two growth rings from bark in Robinia pseudoacacia, but permeated all xylem in fonts ussuriensis. In Robinia pseudoacacia, the vessels of heart wood and intermediate wood were not stained because of tyloses.

• Journal of Plant Biology
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• v.3 no.2
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• pp.29-34
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• 1960

From the view point of phytopathological anatomy, the author has tried to study the effect of the shoot cluster disease virus on the internal structure of vascular tissues of chinese date tree (Ziziphus jujuba var. inermis Rehd.) comparing healthy checks and diseased plants. The materials were collected at the several sites, Kumgock-Ri, Masuc-Ri, Kyungi-Do, and near the campus of Korea University and around the area of Chongam-Dong, Seoul City, from August 15th to September 5th 1959. The leaf materials of healthy and diseased plants are fixed and aspirated in two kinds of killing solutions, formalin-acetic acid alcohol solution and Craf III solution. Sections were cut at 5-10$\mu$ thickness and stained with the double staining reagents of safranin and fast green. In this experiment the author has observed that there are marked structural changes in the infected plants in contrast of healthy checks. As figures 3-7 show that the following characteric changes have taken place on infected plants: 1) the arrangement of irregularly developed sieve elements in phloem, 2) the degeneration of phloem elements, 3) the irregular arrangement of epidermis in mid-vein, 4) more necrosis is observed among the parenchymatous cells, 5) abundant accumulatin of starch grains in parenchymatous cells, . In contrast to the above irregularities caused by the virus disease, the healthy checks appear normal structures as shown in figures 1 and 2. In adding to the all features noted above, the author could also observe an interesting feature that the xylem elements in mid-vein vascular bundle tissues are considerably disorganized to show the unspecialized vessel elements, the irregularly arranged xylem elements. However, this kind of irregularities which occur in xylem under the virus infection has not been reported previously. The features noted on the internal structure of vascular bundle under the condition of infection by the shoot cluster disease on chinese date trees appear to be more or less closely similar to the symptoms of the bunchy-top of banana and the yellow dwarf disease of barley in respect to the fact that whether phloem necrosis takes place as a primary symptom or a secondary symptom. In all these disease, primary histological changes of hypoplasia and hypertrophy are preceeded by the necrosis of phloem.

• Korean journal of applied entomology
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• v.23 no.4 s.61
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• pp.215-220
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• 1984

Mulberry dwarf, paulownia witches' broom, jujube witches' broom, and sumach witches' broom are known to be associated with mycoplamalike organisms(MLO) in Korea. Simple microscopic detection of MLO infection in these plants was attempted. Periwinkle plant was also tested. Application of $0.2\%\;and\;0.4\%$ solution of Dienes' stain gave diagnoatic value for MLO-induced diseases of periwinkle and mulberry. Among the various plant parts examined, young herbaceous stem just below the apical part gave the best result. Density of staining reaction was proportional to disease severity. Longitudial sections were superior to transverse sections in confirming MLO infection by staining. Light source without blue filter was useful for increasing the color contrast between sieve tube and xylem vessel and for eliminating misinterpretation. Paulownia, jujube, and sumach samples gave no clear difference in staining reaction between healthy and diseased sections even when various modifications of Dienes' staining procedure were tried.

• The Korea Journal of Herbology
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• v.30 no.4
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• pp.65-69
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• 2015

Objectives : The root ofCyanchum wifordii(CW) had been used as herbal medicine, 'Baeksuo', in Korea. However, the root ofCynanchum auriculatum(CA) had also been mis-used as 'Baeksuo' in herbal markets, due to its morphological similarity with CW. This study aimed to compare discriminative features and establish the identification-keys between two herbal medicines using microscopic methods.Methods : Microscopic preparations including fixation, rapid dehydration, paraffin infiltration, paraffin embedding, sectioning, mounting, and staining, were performed and the microscopic features between CW and CA samples were compared.Results : The starch grain of parenchymal cells, the amounts of calcium oxalate crystals, cell sizes, and the shape of phloem were not different between CW and CA samples. However, the clusters of stone cells which were conglomerated at outer cortex were apparently observed in CA sample (4~8 layers of stone cells), but not in CW sample (2~4 layers of stone cells, or not). Xylem of CW consisted of several vessels and fiber bundles which were formed as linear shapes and discontinuous radial lines, while that of CA, in which radial lines was not observed, were shaped as a wedge and scattered near cambium with comparatively few fiber bundles enclosed vessels. Especially, in the xylem of CA, fiber bundles located towards cambium were relatively more developed than those located outward cambium.Conclusions : The identification-keys established in this study would be helpful to discriminate microscopic features between CW and CA.

• Journal of Ginseng Research
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• v.28 no.3
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• pp.149-156
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• 2004

The objectives of this study were to investigate the morphology of mycorrhizal roots, and the effects of root age and soil texture on the mycorrhizal infection in ginseng (Panax ginseng C. A. Meyer) growing in Korea. Ginseng roots at ages of two to six years were collected from fields in late June. Their infection by arbuscular mycorrhizal fungi(AMF) was studied by clearing the roots and staining fungal hyphae with trypan blue. Root infection varied greatly depending on the developmental stages of young roots. Young tertiary roots, in diameter of smaller than 0.8 mrn, formed during the current growing season had root hairs and were frequently and in some cases heavily infected by AMF. Hyphal coils and arbuscules were abundant, while vesicles were rarely observed. Older secondary or tertiary roots in diameter of bigger than 1.0 mm with fully differentiated primary xylem formed during the previous growing season had no root hairs, and were not infected at all. The rates of mycorrhizal infection in the young tertiary roots were not affected by the age of the ginseng plants, suggesting that fungal populations might have not much changed during the aging of the cultivated fields up to six years. The differences in the infection rates among the different ages of ginseng were caused by differences in the amount of young tertiary roots in the samples. Soil texture, either sandy loam or clay loam, did not affect the rate of root infection. There were large variations in the infection rates among the different farms and locations within a farm. It strongly suggested that infection rates of the ginseng roots by AMF would be influenced by the practice of the farmers, possibly by avoiding consecutive planting, introduction of new topsoil, and the ways of handling the soil before transplanting the ginseng, such as fumigation or sterilization that might have affected indigenous inoculum sources of the AMF.

• The Korea Journal of Herbology
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• v.26 no.1
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• pp.1-6
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• 2011

Objectives : Due to morphological similarity, many substitutes and counterfeits have been used as Moktong for thousands of years. This study was designed to determine discriminative criteria for identifying medicinal herbs used as Moktong. Methods : Akebia quinata, A. trifoliata, Clematis armandii, and Aristolochia manshuriensis were collected in various regions. Samples were authenticated and examined by observation and microscopy with paraffin embedding and triple staining using the modified Ju staining method. Results : Three different types of features to establish discriminative criteria for four kinds of Moktong were identified. Botanical features include differences in phyllotaxy, leaf shape, and caulescent. External morphological features were arrangement and size of fine holes(xylem), and overall shape of the section. Internal morphological features include the number of medullary rays, shape of the pitch, type of tissues rounding pitch, appearance of annual rings, shape and amount of crystals(calcium-oxalate), and the appearance of cork cambium. Further details(e.g. identification keys) are in the article. Conclusions : These criteria could provide an effective method for identifying numerous kinds of Moktong distributed in markets throughout northeast Asian nations.

• Journal of the Korean Wood Science and Technology
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• v.36 no.4
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• pp.84-92
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• 2008

Anatomical and physical characteristics of Pinus densiflora woods damaged by forest fire at Yangyang-gun, Kangwon-do were investigated in present study. Even though the bark was severly carbonized, no trace of carbonization in the xylem was found. The amount of resin exudation was different by the degree of fire damage. Green moisture content of sapwood in the damaged wood was lower than that in the sound wood, but vice versa in the heartwood. Green density of heartwood in the damaged wood was higher than that in the sound wood, but there was no significant difference in sapwood. Cambial activities were found stopped in the severely damaged wood but were identified in the lightly damaged wood. In safranine staining process, epithelial and ray parenchyma cell walls in the damaged woods showed darker hue than those in the sound wood. Granular substances were observed in the lumina of ray parenchyma and epithelial cells of the severely damaged wood. Relative crystallinity of the outermost growth ring in the severely damaged wood was slightly higher than that in the sound wood.